Cross‐sectional study on allergic sensitization of Austrian adolescents using molecule‐based IgE profiling

Stemeseder, Teresa; Klinglmayr, Eva; Moser, Stephanie; Lueftenegger, Lisa; Lang, Roland; Himly, Martin; Oostingh, Gertie Janneke; Zumbach, Joerg; Bathke, Arne C.; Hawranek, Thomas; Gadermaier, Gabriele

doi:10.1111/all.13071

Cited by 45 publications

(79 citation statements)

References 38 publications

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“…In line with the “hygiene hypothesis,” the beneficial effect of growing up on a farm was confirmed within this work [5,6,7]. The difference was particularly pronounced regarding sensitization to PR-10 and grass pollen allergens, which might relate to high pollen exposure among farm children and/or the general high sensitization rate towards these sources [8]. …”

Section: Discussionsupporting

confidence: 80%

“…In this cohort of 501 adolescents, a general sensitization rate of 53.5% was detected by means of ImmunoCAP ISAC® analysis [8]. Additional data regarding the study population is displayed in Figure 1a.…”

Section: Resultsmentioning

confidence: 99%

“…Among analyzed lifestyle aspects, only smoking showed a significant difference for IgE sensitization and frequently involved allergen molecules with very low sensitization rates [8]. An association of smoking and increased incidence of asthma and wheeze has been shown before [30], but an association between never smoking and a higher risk of atopy has also been reported [12,13].…”

Section: Discussionmentioning

confidence: 99%

“…Serum samples from a cohort of 501 nonselected Austrian adolescents (aged 12-21 years) were analyzed for IgE to 112 single components using the ImmunoCAP ISAC® [8]. Personal data and lifestyle information were assessed by a questionnaire and correlated with sensitization data.…”

Section: Methodsmentioning

confidence: 99%

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Influence of Intrinsic and Lifestyle Factors on the Development of IgE Sensitization

Stemeseder¹,

Klinglmayr²,

Moser³

et al. 2017

Int Arch Allergy Immunol

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Background: IgE sensitization is a prerequisite for the development of allergic symptoms. The investigation of factors influencing the development of IgE is therefore crucial for understanding the onset of allergic diseases. Methods: This epidemiological study investigated personal, intrinsic, and lifestyle factors in a nonselected cohort of 501 Austrian adolescents (aged 12-21 years). IgE levels to 112 allergen molecules were analyzed in the serum of participants using the ImmunoCAP ISAC®. Allergic sensitization, IgE levels to single allergens, and ISAC score sums were correlated with results obtained from a questionnaire. Results: In this adolescent cohort, male participants showed a higher sensitization frequency (56.8%) compared to females (50.9%) and significantly increased IgE levels to profilins. Underweight subjects demonstrated a stronger IgE sensitization. Family size inversely correlated with IgE levels to PR-10 allergens, and predominately paternal allergies were a predictive factor for IgE sensitization in the children. Vaccination, breastfeeding, and delivery mode showed no influence, while a highly protective effect was observed for growing up on a farm. Of all of the investigated lifestyle factors, only smoking significantly influenced the risk for IgE development. Participants with moderate frequencies of colds showed increased sensitization levels. Conclusion: A hereditary predisposition and lifestyle factors such as a farming environment, smoking, family size, body weight, or frequency of colds significantly influenced the development of allergen-specific IgE in this cohort of adolescents.

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Section: Discussionsupporting

confidence: 80%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Influence of Intrinsic and Lifestyle Factors on the Development of IgE Sensitization

Stemeseder¹,

Klinglmayr²,

Moser³

et al. 2017

Int Arch Allergy Immunol

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“…Plantago lanceolata (English plantain) represents the most important allergen source among weeds from the Plantaginaceae family [1][2][3]. Plantain pollen is usually not included in routine allergy diagnosis, and its clinical relevance may thus be underestimated in some regions [1,4].…”

Section: Introductionmentioning

confidence: 99%

Do <b><i>Plantago lanceolata</i></b> Skin Prick Test-Positive Patients Display IgE to Genuine Plantain Pollen Allergens? Investigation of Pollen Allergic Patients from the North-East of France

Stemeseder¹,

Metz-Favre²,

Blay³

et al. 2018

Int Arch Allergy Immunol

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Background: English plantain (Plantago lanceolata) is an important weed pollen allergen source triggering allergic symptoms during summer. To elucidate genuine versus cross-reactive sensitization, we investigated IgE reactivity patterns and inhibition capacities of plantain-sensitized patients. Methods: Sera of 35 rhinoconjunctivitis patients from the north-east of France with positive skin prick tests (SPT) to Plantago lanceolata pollen were tested with clinically relevant allergen sources using ELISA, ImmunoCAP, and immunoblot inhibition. Results: The patients were multisensitized with additional reactivity to grass (94.3%), ash (74.3%), birch (71.4%), and mugwort (55.2%) pollen in SPT. Sensitization prevalence to allergen molecules was 34.3% (Pla l 1), 94.3% (Phl p 1/5), 60.0% (Ole e 1), 65.7% (Bet v 1), 37.1% (profilin), and 40.0% (CCD). In immunoblot, IgE reactivity to plantain pollen was inhibited with relevant pollen extracts and purified rPla l 1. Two sera did not reveal any IgE cross-reactivity, while reactivity to plantain was efficiently inhibited by grass pollen in the sera of 10 patients. The sera from 17 different patients could be inhibited by grass, birch, or ash pollen to varying degrees. Thus, only 37.1% of our patients demonstrated true plantain pollen sensitization, while 62.9% were solely positive due to IgE cross-reactive molecules from other clinically relevant pollen. Conclusions: Plantain pollen-sensitized patients are multi-reactors demonstrating varying and complex IgE-reactivity profiles. In vivo and in vitro tests using extracts are typically blurred due to the presence of homologous allergens or CCD in grass, birch, or ash pollen. So far, Pla l 1 represents the only indicative marker allergen for the diagnosis of genuine plantain pollen sensitization.

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Prevalence of sensitization to molecular food allergens in Europe: A systematic review

Lisik

Ioannidou

Spolidoro

et al. 2022

Clinical & Translational All

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Background Recent reports indicate that the prevalence of food allergy is increasing, but accurate estimates remain a challenge due to cross‐reactivity and limited use of precise diagnostic methods. Molecular allergy diagnostics, in which sensitization to individual molecular allergens is measured, is emerging as a promising tool for evaluation of sensitization profiles. In this systematic review, we summarized estimates of prevalence of sensitization to molecular food allergens in the general population in Europe. Methods Following a protocol prospectively registered with the International Prospective Register of Systematic Reviews (PROSPERO; reference CRD42021266657), we searched seven databases with no restrictions on publication date or language. Two reviewers independently screened the literature, extracted data, and appraised the risk of bias in the included studies. The findings were synthesized narratively. Results From 4776 de‐duplicated records, five studies, with low to moderate overall risk of bias, were included. Forty‐six molecular allergens from 18 foods were investigated. Overall, the prevalence of sensitization was low, particularly for major allergens, and non‐existent for 10 molecular allergens (0% [95% CI 0–0.8]). The highest prevalence was seen for PR‐10 proteins, such as Cor a 1.04 (13.6% [95% CI 10.9–16.9]). Conclusions Available data, primarily from North‐western Europe, indicate that sensitization to molecular food allergens is overall low. The highest estimates were found for cross‐reactive PR‐10 proteins. There were not enough studies to discern regional differences or perform meta‐analysis, highlighting the need for more population‐representative studies in order to elucidate patterns of sensitization to molecular food allergens in Europe.

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Cross‐sectional study on allergic sensitization of Austrian adolescents using molecule‐based IgE profiling

Cited by 45 publications

References 38 publications

Influence of Intrinsic and Lifestyle Factors on the Development of IgE Sensitization

Influence of Intrinsic and Lifestyle Factors on the Development of IgE Sensitization

Do <b><i>Plantago lanceolata</i></b> Skin Prick Test-Positive Patients Display IgE to Genuine Plantain Pollen Allergens? Investigation of Pollen Allergic Patients from the North-East of France

Prevalence of sensitization to molecular food allergens in Europe: A systematic review

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