Complexity of polysomal polyadenylated RNA in mouse whole brain and cortex

Croizat, Bernard; Berthelot, Francis; Felsani, Armando; Gros, François

doi:10.1016/0014-5793(79)81267-3

Cited by 12 publications

(10 citation statements)

References 15 publications

(1 reference statement)

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“…PERT-hybridization conditions (Kohne et al, 1977) were found to be required to maintain the integrity of single-strand plasmid. The intent of the hybridization was to remove most of the sequences from high and medium abundance classes, or about 70% of the normal cDNA clones (Croizat et al, 1979). Lower abundance sequences should a bed a bed a bed abed 28s.…”

Section: Resultsmentioning

confidence: 99%

“…In our experiment it was estimated that the cDNA probe must constitute at least 0.0 1% of the total probe mass to produce a colony hybridization signal above background. RNA species of lower abundance, which constitute the majority of expressed genes in the cerebellum (Croizat et al, 1979), may be expected to be undetectable as cDNA clones. A second limitation derives from the required use of tissue mRNA, in which cell-type-specific mRNA species become diluted.…”

Section: Discussionmentioning

confidence: 99%

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cDNA cloning and characterization of three genes uniquely expressed in cerebellum by Purkinje neurons

1988

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The characteristics that distinguish the different neuronal cell types of an organism are believed to be primarily determined by unique patterns of cellular gene expression. The identification of cell-type specific molecules should therefore provide a good basis for understanding the biology of specific neuron types. In this paper, we describe the isolation of cDNA clones corresponding to mRNA uniquely expressed by Purkinje cells in mature mouse cerebellum. Three cDNA clones were selected from a library of normal mouse cerebellar cDNA by virtue of their failure to hybridize to mRNA sequences from the cerebella of Purkinje cell degeneration (pcd) mice. The cDNA clones were shown by in situ and Northern hybridization to correspond with mRNA present in Purkinje cells but absent or at low levels in other cell types of the cerebellum. By sequence analysis, clone PCD29 was determined to encode the calcium-binding protein calbindin-D28K. Clones PCD5 and PCD6 encode previously undescribed proteins of 99 and greater than 500 amino acids, respectively. All 3 PCD clones hybridized to mouse mRNA from sources other than cerebellum; clone PCD5 was found to have the most restricted expression, as it hybridized only to mRNA from cerebellum and eye. To define potential correlations between the PCD clones and mutations in the mouse genome known to affect Purkinje cells, clones PCD5, PCD6, and PCD29 were localized to mouse chromosomes 8, 6, and 4, respectively.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

cDNA cloning and characterization of three genes uniquely expressed in cerebellum by Purkinje neurons

1988

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“…Hybridization studies of brain mRNA revealed that this organ transcribes a large number of nonabundant mRNA sequences. These represent only a small part of the mass of brain mRNA but constitute most of its sequence complexity (Bantle and Hahn, 1976;Croizat et al, 1979). This is generally accounted for by the heterogeneity of brain cell types (Kaplan et al, 1978).…”

Section: Discussionmentioning

confidence: 99%

Degranulation of Rat Cerebellum Induces Selective Variations in Gene Expression

Eliyahu

Soreq

1982

Journal of Neurochemistry

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Selective variations in the composition of poly(A)-containing mRNA were found to be induced in the rat cerebellum by X-irradiation. mRNA populations prepared from normal and X-irradiated rat cerebella at different stages of their development displayed equal efficiencies when translated in vitro in reticulocyte lysates. Specific differences were revealed, however, when the labeled translation products of both mRNA preparations were subjected to two-dimensional gel electrophoresis followed by fluorography of the dried gels. Of more than 100 polypeptide products, several showed marked intensity differences, indicating changes in the abundance of their directing mRNA species. These differences appear both in developing and in mature cerebellar mRNA, and the extent of modification in mRNA is much higher than the consequent changes in the composition of proteins in the irradiated cerebellum. The degranulation-induced modifications in levels of specific cerebellar mRNA species can be used to identify proteins whose biosynthesis depends on the presence of interneurons.

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“…Cell culture Culture of the mouse neuroblastoma clonal line NIE 115 was carried out as previously described (Croizat et al, 1979). When cultured in growth medium supplemented with 1% (w/v) 1-methylcyclohexane carboxylic acid, the cells acquire a neuronal phenotype.…”

Section: Methodsmentioning

confidence: 99%

Multiple mRNAs encode peripherin, a neuronal intermediate filament protein.

et al. 1989

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Three cDNA clones of 1.6 (3u), 1.2 (5g) and 0.6 (5b) kbp with probes covering the 3' end of the two unexpected regions show that three distinct mRNAs correspond to the three cDNAs. Moreover, three peripherin products, two minor 61 and 56 kd products in addition to the major 58 kd peripherin, are observed when poly(A)+ RNA is in vitro translated, the 61 kd peripherin being translated from the 3u-selected RNA. The three RNAs originate from alternative splicing of a unique peripherin gene, thus generating polymorphism of peripherin.

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Complexity of polysomal polyadenylated RNA in mouse whole brain and cortex

Cited by 12 publications

References 15 publications

cDNA cloning and characterization of three genes uniquely expressed in cerebellum by Purkinje neurons

cDNA cloning and characterization of three genes uniquely expressed in cerebellum by Purkinje neurons

Degranulation of Rat Cerebellum Induces Selective Variations in Gene Expression

Multiple mRNAs encode peripherin, a neuronal intermediate filament protein.

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