Multiple mRNAs encode peripherin, a neuronal intermediate filament protein.

Landon, Françoise; Lemonnier, Marguerite; Bénarous, Richard; Huc, Claude; Fiszman, Marc; Gros, François; Portier, Marie‐Madeleine

doi:10.1002/j.1460-2075.1989.tb03564.x

Cited by 77 publications

(58 citation statements)

References 40 publications

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“…Other studies (Djabali et al, unpublished observations) show that peripherin and NFL are coexpressed in every neuron of primary cultures either from the dorsal root ganglia or from the sympathetic ganglia; this means that peripherin expression is a general feature of the diverse classes of neurons that we have listed under (2) and do not allow to differentiate either among neuronal types in the autonomous nervous system or among the sensory neurons. From the localization of the phosphorylated sites on the amino-terminal part of its molecule , from the nucleotide sequence of cDNAs encoding either rat or mouse peripherin (Leonard et al, 1988;Parysek et al, 1988;Landon et al, 1989), and from the sequence of the rat peripherin gene (Thompson and Ziff, 1989), this protein is a type III IFP, as vimentin and desmin are; these 2 proteins have been found to be linked to the nuclear membrane through lamin B by their carboxy-terminal end and to the plasmic mem- Figure 12. Expression of peripherin in the olfactory nerve fibers.…”

Section: Discussionmentioning

confidence: 99%

Differential expression of two neuronal intermediate-filament proteins, peripherin and the low-molecular-mass neurofilament protein (NF-L), during the development of the rat

et al. 1990

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The expression of peripherin, an intermediate filament protein, had been shown by biochemical methods to be localized in the neurons of the PNS. Using immunohistochemical methods, we analyzed this expression more extensively during the development of the rat and compared it with that of the low-molecular-mass neurofilament protein (NF-L), which is expressed in every neuron of the CNS and PNS. The immunoreactivity of NF-L is first apparent at the 25-somite stage (about 11 d) in the ventral horn of the spinal medulla and in the posterior part of the rhombencephalon. The immunoreactivity of peripherin appears subsequently, first colocalized with that of NF-L. Both immunoreactivities then spread out along rostral and caudal directions, but whereas the immunoreactivity of NF-L finally becomes noticeable in every part of the nervous system, that of peripherin remains localized to (1) the motoneurons of the ventral horn of the spinal medulla; (2) the autonomic ganglionic and preganglionic neurons; and (3) the sensory neurons. These results demonstrate that, in the neurons that originate from migrating neural crest cells, the immunoreactivities of peripherin and of NF-L become apparent only when they have reached their destination. The results also show that peripherin is expressed more widely than has been previously observed and that this protein occurs in neuronal populations from different lineages (neural tube, neural crest, placodes) with different functions (motoneurons, sensory and autonomic neurons). The common point of these neurons is that they all have axons lying, at least partly, at the outside of the axis constituted by the encephalon and the spinal medulla; this suggests that peripherin might play a role in the recognition of the axonal pathway through the intermediary of membrane proteins.

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Section: Discussionmentioning

confidence: 99%

Differential expression of two neuronal intermediate-filament proteins, peripherin and the low-molecular-mass neurofilament protein (NF-L), during the development of the rat

et al. 1990

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“…Clearly, it reacts with the lightest neurofilament protein (NF-L), vimentin and its derived peptides [33], the different isoforms of peripherin [34], GFAP, desmin and every cytokeratin present in the preparation from human oral epithelia. These data initially led us to postulate that ME 101 behaves like the previously described IFA antibody elicited by Pruss el al.…”

Section: Selection Of Hybridomasmentioning

confidence: 99%

A new monoclonal antibody recognizing the amino‐terminal consensus sequence of vertebrate intermediate filament proteins

et al. 1989

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The mouse monoclonal antibody ME 101 raised against human peripherin, an intermediate filament protein (IFP) specific to well defined neuronal populations, recognizes all the major classes of vertebrate IFP in immunoblotting assays. Desmin, GFAP, vimentin, peripherin and the lightest neurofilament protein (NF-L) were cleaved into carboxy-and amino-terminal halves by N-chlorosuccinimide at their unique trytophan residue. Whereas the antibody directed against the epitope common to every IFP (intermediate filament antigen or IFA) and located on the carboxy-terminal end of the rod domain recognizes the carboxy-terminal half, the ME 101 antibody, as the present study illustrates, recognizes specifically the amino-terminal half. From the amino acid sequence data of IFP, it is deduced that the cognate epitope is localized on the amino-terminal part of coil la.

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“…In mice, humans, and other mammals, the production of peripherin is limited to the PNS and subsets of CNS neurons (e.g., the spinal motor neurons, neurons of sensory origin, and small interneurons in the cortex and hippocampus) (20). Pathologically, peripherin has been found to be associated with pathological aggregates in the motor neurons of patients with amyotrophic lateral sclerosis (21)(22)(23), and in the murine model of this disease, i.e., the transgenic mice expressing a mutant of the superoxide dismutase-1 (SOD1 G37R ) (24,25).…”

Section: Discussionmentioning

confidence: 99%

“…It has been hypothesized that a defect in the clearance of apoptotic nervous and ␤ cells during this period of time may be crucial in the development of the autoimmune disease in NOD mice (38,39). Peripherin is a key factor in neuronal development (20), and its production, unlike other neurofilaments, is up-regulated after nerve injury and by proinflammatory cytokines, such as IL-6 (40,41). Thus, we hypothesize that a defect in the clearance process causes an incipient inflammation that up-regulates the production of peripherin in the pancreatic nervous system and/or ␤ cells and favors the autoreactive response in this proinflammatory context.…”

Section: Discussionmentioning

confidence: 99%

“…5A) differentiates Per56 from Per58 and Per61 isoforms. In vivo production levels of Per58 are very high, whereas those of Per61 and Per56 are very low (20). Therefore, to investigate which peripherin isoform was targeted by our mAbs, three mammalian expression plasmids encoding the peripherin isoforms Per58, Per56, or Per61 were transfected into SW13 vim(Ϫ) cells, and the reactivity of mAbs was analyzed (Fig.…”

Section: Most Anti-pns Mabs Recognize a Dominant Epitope Mapping At Tmentioning

confidence: 99%

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Peripherin Is a Relevant Neuroendocrine Autoantigen Recognized by Islet-Infiltrating B Lymphocytes

Puertas

Carrillo

Pastor

et al. 2007

The Journal of Immunology

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Most of our knowledge of the antigenic repertoire of autoreactive B lymphocytes in type 1 diabetes (T1D) comes from studies on the antigenic specificity of both circulating islet-reactive autoantibodies and peripheral B lymphocyte hybridomas generated from human blood or rodent spleen. In a recent study, we generated hybridoma cell lines of infiltrating B lymphocytes from different mouse strains developing insulitis, but with different degrees of susceptibility to T1D, to characterize the antigenic specificity of islet-infiltrating B lymphocytes during progression of the disease. We found that many hybridomas produced mAbs restricted to the peripheral nervous system (PNS), thus indicating an active B lymphocyte response against PNS elements in the pancreatic islet during disease development. The aim of this study was to identify the autoantigen recognized by these anti-PNS mAbs. Our results showed that peripherin is the autoantigen recognized by all anti-PNS mAbs, and, therefore, a relevant neuroendocrine autoantigen targeted by islet-infiltrating B lymphocytes. Moreover, we discovered that the immune dominant epitope of this B lymphocyte immune response is found at the C-terminal end of Per58 and Per61 isoforms. In conclusion, our study strongly suggests that peripherin is a major autoantigen targeted during T1D development and poses a new question on why peripherin-specific B lymphocytes are mainly attracted to the islet during disease.

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Multiple mRNAs encode peripherin, a neuronal intermediate filament protein.

Cited by 77 publications

References 40 publications

Differential expression of two neuronal intermediate-filament proteins, peripherin and the low-molecular-mass neurofilament protein (NF-L), during the development of the rat

Differential expression of two neuronal intermediate-filament proteins, peripherin and the low-molecular-mass neurofilament protein (NF-L), during the development of the rat

A new monoclonal antibody recognizing the amino‐terminal consensus sequence of vertebrate intermediate filament proteins

Peripherin Is a Relevant Neuroendocrine Autoantigen Recognized by Islet-Infiltrating B Lymphocytes

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