volume 66, issue 8, P4901-4908 1992
DOI: 10.1128/jvi.66.8.4901-4908.1992
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Abstract: We present evidence that the formation of NP-P and P-L protein complexes is essential for replication of the genome of Sendai defective interfering (DI-H) virus in vitro, using extracts of cells expressing these viral proteins from plasmids. Optimal replication of DI-H nucleocapsid RNA required extracts of cells transfected with critical amounts and ratios of each of the plasmids and was threeto fivefold better than replication with a control extract prepared from a natural virus infection. Extracts in which N…

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