Complex regulation of CDKs and G1 arrest during the granulocytic differentiation of human myeloblastic leukemia ML-1 cells

Shimizu, Takaki; Awai, Nao; Takeda, Ken

doi:10.1038/sj.onc.1203821

Cited by 16 publications

(10 citation statements)

References 45 publications

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“…The study by Hsieh et al (2000) measured cell cycle regulators in nontransformed murine proerythroblasts undergoing in vitro di erentiation in response to erythropoietin. The ®ndings of this study were largely consistent with those of Matushansky et al (2000b), as were changes observed in a myeloid di erentiation model (Shimizu et al, 2000). In contrast, Tamir noted a di erent pattern of interactions in the HB60-5 cell line, which grows in SCF plus erythropoietin and di erentiates upon removal of SCF (Figure 3b).…”

Section: Cell Cycle Regulators and Erythropoiesissupporting

confidence: 84%

Cell cycle regulators and hematopoiesis

Steinman

2002

Oncogene

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Section: Cell Cycle Regulators and Erythropoiesissupporting

confidence: 84%

Cell cycle regulators and hematopoiesis

Steinman

2002

Oncogene

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“…In contrast to p27, these data showed increased p21 levels induced by Akt. The function of p21 in cell cycle is complex, and new evidence suggests that the main function during cell cycle progression appears to be ensuring appropriate cdk4 activation and cell survival (31)(32)(33)(34)(35). The role of p21 in the proliferative phenotype is unclear, but it is possible that the increase in the number cdk4 complex proteins (cyclin D1, D2, and p21) stabilizes and prevents p21 degradation.…”

Section: Discussionmentioning

confidence: 99%

Akt Induces β-Cell Proliferation by Regulating Cyclin D1, Cyclin D2, and p21 Levels and Cyclin-Dependent Kinase-4 Activity

et al. 2006

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Proliferation is the major component for maintenance of ␤-cell mass in adult animals. Activation of phosphoinositide 3-kinase/Akt-kinase pathway is a critical regulator of ␤-cell mass. Pancreatic ␤-cell overexpression of constitutively active Akt in mice (caAkt Tg ) resulted in marked expansion of ␤-cell mass by increase in ␤-cell proliferation and size. The current studies provide new insights into the molecular mechanisms involved in ␤-cell proliferation by Akt. Proliferation of ␤-cells in caAkt Tg was associated with increased cyclin D1, cyclin D2, and p21 levels and cyclindependent kinase-4 (cdk4) activity. To determine the role of cdk4 in ␤-cell proliferation induced by Akt, we generated caAkt Tg mice that were homozygous, heterozygous, or nullizygous for cdk4. The results of these studies showed that deletion of one cdk4 allele significantly reduced ␤-cell expansion in caAkt Tg mice by decreased proliferation. CaAktTg mice deficient in cdk4 developed ␤-cell failure and diabetes. These experiments suggest that Akt induces ␤-cell proliferation in a cdk4-dependent manner by regulation of cyclin D1, cyclin D2, and p21 levels. These data also indicate that alteration in levels of these cell cycle components could affect the maintenance of ␤-cell mass in basal states and the adaptation of ␤-cells to pathological states resulting in diabetes. Diabetes 55:318 -325, 2006 T he defects that result in diabetes are diverse, but pancreatic ␤-cell failure is a major component of this disease (1,2). Pancreatic ␤-cell mass results from a dynamic balance of neogenesis, proliferation, cell size, and apoptosis (3). Recent data using cell lineage tracing experiments suggest that replication is a major component involved in maintenance of ␤-cell mass in adult animals (4). The molecular mechanisms involved in regulation of ␤-cell mass are unknown. Several lines of evidence suggest that activation of phosphoinositide 3-kinase (PI 3-kinase) signaling pathway plays critical roles in regulation of ␤-cell mass and function (5-10).One of the major targets of PI 3-kinase is the serinethreonine kinase Akt, the cellular homolog of the viral oncogene v-Akt (11). Akt is a convergent point of several growth signals induced by growth factors, insulin, and gut incretins. Transgenic mice overexpressing a constitutively active form of Akt in islet ␤-cells (caAkt Tg ) have provided further evidence for the role of this kinase in islet physiology (12,13). These mice exhibit striking increases in ␤-cell mass, proliferation, neogenesis, and cell size. Akt regulates cell cycle progression by alterations in levels and subcellular distribution of components of the cell cycle machinery (14). The molecules and the mechanisms involved in the regulation of cell cycle by growth signals mediated by Akt in pancreatic ␤-cells are ill defined.Insight into the mechanisms involved in the regulation of cell cycle in ␤-cells have been provided by experiments in cyclin-dependent kinase-4 (cdk4)-deficient mice. These mice exhibited growth retardation and diabetes du...

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“…43 In contrast, p21 Cip1 can bind to and inhibit kinase activities of Cdk2/cyclin A and Cdk2/cyclin E complexes from the late G 1 until the S phase. 44,45 …”

Section: Cell Cycle Overviewmentioning

confidence: 99%