Complex genetic control of non-target-site-based resistance to herbicides inhibiting acetyl-coenzyme A carboxylase and acetolactate-synthase in Alopecurus myosuroides Huds.

Petit, Christelle; Duhieu, Bastien; Boucansaud, Karelle; Délye, Christophe

doi:10.1016/j.plantsci.2010.03.007

Cited by 92 publications

(104 citation statements)

References 34 publications

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“…In these species, the resistance to each herbicide is frequently regulated by multiple genes (e.g. Powles, 2005a, 2005b;Petit et al, 2010;Busi et al, 2011Busi et al, , 2013. This discrepancy can be explained by the mode of pollination.…”

Section: Discussionmentioning

confidence: 99%

Cytochrome P450 CYP81A12 and CYP81A21 Are Associated with Resistance to Two Acetolactate Synthase Inhibitors in Echinochloa phyllopogon

et al. 2014

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Previous studies have demonstrated multiple herbicide resistance in California populations of Echinochloa phyllopogon, a noxious weed in rice (Oryza sativa) fields. It was suggested that the resistance to two classes of acetolactate synthase-inhibiting herbicides, bensulfuron-methyl (BSM) and penoxsulam (PX), may be caused by enhanced activities of herbicide-metabolizing cytochrome P450. We investigated BSM metabolism in the resistant (R) and susceptible (S) lines of E. phyllopogon, which were originally collected from different areas in California. R plants metabolized BSM through O-demethylation more rapidly than S plants. Based on available information about BSM tolerance in rice, we isolated and analyzed P450 genes of the CYP81A subfamily in E. phyllopogon. Two genes, CYP81A12 and CYP81A21, were more actively transcribed in R plants compared with S plants. Transgenic Arabidopsis (Arabidopsis thaliana) expressing either of the two genes survived in media containing BSM or PX at levels at which the wild type stopped growing. Segregation of resistances in the F2 generation from crosses of R and S plants suggested that the resistance to BSM and PX were each under the control of a single regulatory element. In F6 recombinant inbred lines, BSM and PX resistances cosegregated with increased transcript levels of CYP81A12 and CYP81A21. Heterologously produced CYP81A12 and CYP81A21 proteins in yeast (Saccharomyces cerevisiae) metabolized BSM through O-demethylation. Our results suggest that overexpression of the two P450 genes confers resistance to two classes of acetolactate synthase inhibitors to E. phyllopogon. The overexpression of the two genes could be regulated simultaneously by a single trans-acting element in the R line of E. phyllopogon.

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Section: Discussionmentioning

confidence: 99%

Cytochrome P450 CYP81A12 and CYP81A21 Are Associated with Resistance to Two Acetolactate Synthase Inhibitors in Echinochloa phyllopogon

et al. 2014

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“…It is emphasized that the genetic model used in this study is valid at the population level for phenotypes originally selected with a high diclofop-methyl dose and subsequently used to generate F1, F2 and BC lines. However, populations of cross-pollinated species are rarely homogeneous and therefore a higher degree of genetic complexity within a population can exist (Petit et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…The genetic basis of P450-mediated metabolism-based chlortoluron resistance or non-target-site resistance to the ACCase herbicide pinoxaden in A. myosuroides has been shown to be controlled by multiple additive genes (Chauvel, 1991;Petit et al, 2010). Mackenzie et al (1995) claimed polygenetic, quantitatively inherited chlorsulfuron resistance in Lolium perenne.…”

Section: Discussionmentioning

confidence: 99%

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Genetic control of a cytochrome P450 metabolism-based herbicide resistance mechanism in Lolium rigidum

2010

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The dynamics of herbicide resistance evolution in plants are influenced by many factors, especially the biochemical and genetic basis of resistance. Herbicide resistance can be endowed by enhanced rates of herbicide metabolism because of the activity of cytochrome P450 enzymes, although in weedy plants the genetic control of cytochrome P450-endowed herbicide resistance is poorly understood. In this study we have examined the genetic control of P450 metabolism-based herbicide resistance in a well-characterized Lolium rigidum biotype. The phenotypic resistance segregation in herbicide resistant and susceptible parents, F1, F2 and backcross (BC) families was analyzed as plant survival following treatment with the chemically unrelated herbicides diclofop-methyl or chlorsulfuron. Dominance and nuclear gene inheritance was observed in F1 families when treated at the recommended field doses of both herbicides.The segregation values of P450 herbicide resistance phenotypic traits observed in F2 and BC families was consistent with resistance endowed by two additive genes in most cases. In obligate out-crossing species such as L. rigidum, herbicide selection can easily result in accumulation of resistance genes within individuals.

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“…Cases of herbicide-resistant A. fatua have been reported for several European countries such as Belgium, France, Germany, Poland, UK and Turkey [29]. Non-target site resistance is believed to be of polygenic nature, involving several gene loci and to require some generations of sexual reproduction for enrichment of resistance alleles in single plants [30,31]. Avena fatua is mainly self-pollinating, and outcrossing rates are only between 0.05 to 0.08% in wheat and hence contribution of outcrossing to evolution of resistance is assumed to be low [32].…”

Section: Discussionmentioning

confidence: 99%

Winter wheat yield loss in response to Avena fatua competition and effect of reduced herbicide dose rates on seed production of this species

2017

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Avena fatua is one of the most abundant and competitive grass weed species in cereal cropping systems worldwide. Despite its main occurrence in summer annual cereals, A. fatua is suspected to cause significant yield losses in winter wheat as well. Five yield loss experiments were conducted over the course of five years to investigate the impact of A. fatua on winter wheat yield. Two additional field studies were conducted to assess the efficacy of two ALS-inhibiting herbicides as well as of two ACCaseinhibiting herbicides at variable dose rates on A. fatua seed production. Avena fatua caused significant winter wheat yield losses of up to 40% at densities of approximately 250 plants m -2 . Winter wheat yield losses did not differ between years and sites. Relative A. fatua biomass was a better predictor for winter wheat yield loss as A. fatua density and biomass. Three out of four herbicides showed high efficacy against A. fatua, even at reduced dose rates, but A. fatua seed production was not directly related to herbicide efficacy. Seed production was rather influenced by the mode of action and the competitiveness of the winter wheat stand. The results showed that A. fatua can be a serious threat for winter wheat production in the local conditions, leading to high yield losses if not controlled. We did not find a general potential for reducing herbicide dose rates for the control of A. fatua in winter wheat due to variable effects on A. fatua seed production. The potential is highly dependent on the winter wheat competitiveness and herbicide mode of action.

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Complex genetic control of non-target-site-based resistance to herbicides inhibiting acetyl-coenzyme A carboxylase and acetolactate-synthase in Alopecurus myosuroides Huds.

Cited by 92 publications

References 34 publications

Cytochrome P450 CYP81A12 and CYP81A21 Are Associated with Resistance to Two Acetolactate Synthase Inhibitors in Echinochloa phyllopogon

Cytochrome P450 CYP81A12 and CYP81A21 Are Associated with Resistance to Two Acetolactate Synthase Inhibitors in Echinochloa phyllopogon

Genetic control of a cytochrome P450 metabolism-based herbicide resistance mechanism in Lolium rigidum

Winter wheat yield loss in response to Avena fatua competition and effect of reduced herbicide dose rates on seed production of this species

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