Complex and dynamic expression of cadherins in the embryonic marmoset cerebral cortex

Matsunaga, Eiji; Nambu, Sanae; Oka, Mariko; Iriki, Atsushi

doi:10.1111/dgd.12228

Cited by 9 publications

(10 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consistent with previous reports (Basu et al, 2015;Hayano et al, 2014;Krishna et al, 2009;Krishna et al, 2011;Lein et al, 2007;Matsunaga et al, 2015;Paul et al, 2017;Redies, 1997;Tasic et al, 2018), many cadherins were enriched in specific layers and sublayers in the cortex (Fig. 2D).…”

Section: Barseq2 Allows Multiplexed Detection Of Endogenous Mrnas In supporting

confidence: 92%

Integrating barcoded neuroanatomy with spatial transcriptional profiling reveals cadherin correlates of projections shared across the cortex

Sun

Chen

Fischer

et al. 2020

Preprint

View full text Add to dashboard Cite

Functional circuits consist of neurons with diverse axonal projections and gene expression. Understanding the molecular signature of projections requires high-throughput interrogation of both gene expression and projections to multiple targets in the same cells at cellular resolution, which is difficult to achieve using current technology. Here, we introduce BARseq2, a technique that simultaneously maps projections and detects multiplexed gene expression by in situ sequencing. We determined the expression of cadherins and cell-type markers in 29,933 cells, and the projections of 3,164 cells in both the mouse motor cortex and auditory cortex. Associating gene expression and projections in 1,349 neurons revealed shared cadherin signatures of homologous projections across the two cortical areas. These cadherins were enriched across multiple branches of the transcriptomic taxonomy. By correlating multi-gene expression and projections to many targets in single neurons with high throughput, BARseq2 provides a path to uncovering the molecular logic underlying neuronal circuits.

show abstract

Section: Barseq2 Allows Multiplexed Detection Of Endogenous Mrnas In supporting

confidence: 92%

Integrating barcoded neuroanatomy with spatial transcriptional profiling reveals cadherin correlates of projections shared across the cortex

Sun

Chen

Fischer

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Supporting the neural relevance of these Ca 2+ -dependent proteins, CDH4 along with the gene coding for LCC member CDH11 (also OB-cadherin; Figure 5 ) was found to display differential spatial and temporal expression in developing marmoset brain (Matsunaga et al, 2015). In accordance, murine Cdh4 and Cdh11 seem to be essential for the association and migration of neurons during embryogenesis (Kimura et al, 1995; Hertel and Redies, 2011).…”

Section: Discussionmentioning

confidence: 80%

The FOXP2-Driven Network in Developmental Disorders and Neurodegeneration

Oswald

Klöble

Ruland

et al. 2017

Front. Cell. Neurosci.

View full text Add to dashboard Cite

The transcription repressor FOXP2 is a crucial player in nervous system evolution and development of humans and songbirds. In order to provide an additional insight into its functional role we compared target gene expression levels between human neuroblastoma cells (SH-SY5Y) stably overexpressing FOXP2 cDNA of either humans or the common chimpanzee, Rhesus monkey, and marmoset, respectively. RNA-seq led to identification of 27 genes with differential regulation under the control of human FOXP2, which were previously reported to have FOXP2-driven and/or songbird song-related expression regulation. RT-qPCR and Western blotting indicated differential regulation of additional 13 new target genes in response to overexpression of human FOXP2. These genes may be directly regulated by FOXP2 considering numerous matches of established FOXP2-binding motifs as well as publicly available FOXP2-ChIP-seq reads within their putative promoters. Ontology analysis of the new and reproduced targets, along with their interactors in a network, revealed an enrichment of terms relating to cellular signaling and communication, metabolism and catabolism, cellular migration and differentiation, and expression regulation. Notably, terms including the words “neuron” or “axonogenesis” were also enriched. Complementary literature screening uncovered many connections to human developmental (autism spectrum disease, schizophrenia, Down syndrome, agenesis of corpus callosum, trismus-pseudocamptodactyly, ankyloglossia, facial dysmorphology) and neurodegenerative diseases and disorders (Alzheimer’s, Parkinson’s, and Huntington’s diseases, Lewy body dementia, amyotrophic lateral sclerosis). Links to deafness and dyslexia were detected, too. Such relations existed for single proteins (e.g., DCDC2, NURR1, PHOX2B, MYH8, and MYH13) and groups of proteins which conjointly function in mRNA processing, ribosomal recruitment, cell–cell adhesion (e.g., CDH4), cytoskeleton organization, neuro-inflammation, and processing of amyloid precursor protein. Conspicuously, many links pointed to an involvement of the FOXP2-driven network in JAK/STAT signaling and the regulation of the ezrin–radixin–moesin complex. Altogether, the applied phylogenetic perspective substantiated FOXP2’s importance for nervous system development, maintenance, and functioning. However, the study also disclosed new regulatory pathways that might prove to be useful for understanding the molecular background of the aforementioned developmental disorders and neurodegenerative diseases.

show abstract

“…These results suggest that the differential expression of diverse cadherins is involved in primate-specific cortical development (Fig. 7 in Matsunaga et al 2015b).…”

Section: Genetic Characteristics Of the Primate Cortex: A Site For Himentioning

confidence: 77%

Secondary somatosensory cortex of primates: beyond body maps, toward conscious self-in-the-world maps

et al. 2020

Self Cite

View full text Add to dashboard Cite

Recent human imaging studies have revealed the involvement of the secondary somatosensory cortex (SII) in processes that require high-level information integration, such as self-consciousness, social relations, whole body representation, and metaphorical extrapolations. These functions are far beyond its known role in the formation of body maps (even in their most complex forms), requiring the integration of different information modalities in addition to somatosensory information. However, no evidence of such complex processing seems to have been detected at the neuronal level in animal experiments, which would constitute a major discrepancy between human and non-human animals. This article scrutinizes this gap, introducing experimental evidence of human and non-human primates' SII functions set in context with their evolutionary significance and mechanisms, functionally situating the human SII as a primate brain. Based on the presented data, a new concept of a somatocentric holistic self is proposed, represented as a more comprehensive body-in-the-world map in the primate SII, taking into account evolutionary aspects that characterize the human SII and its implication in the emergence of self-consciousness. Finally, the idea of projection is introduced from the viewpoint of cognitive science, providing a logical explanation to bridge this gap between observed behavior and neurophysiological data.

show abstract

Complex and dynamic expression of cadherins in the embryonic marmoset cerebral cortex

Cited by 9 publications

References 51 publications

Integrating barcoded neuroanatomy with spatial transcriptional profiling reveals cadherin correlates of projections shared across the cortex

Integrating barcoded neuroanatomy with spatial transcriptional profiling reveals cadherin correlates of projections shared across the cortex

The FOXP2-Driven Network in Developmental Disorders and Neurodegeneration

Secondary somatosensory cortex of primates: beyond body maps, toward conscious self-in-the-world maps

Contact Info

Product

Resources

About