Completion of the swine genome will simplify the production of swine as a large animal biomedical model

Purpose To study the effects of serum and growth factors on propagation of porcine male germline stem cells (MGSCs) in vitro and develop a culture system for these stem cells. Methods Fresh testicular cells from neonatal piglets were obtained by mechanical dissociation and collagenase-trypsin digestion. After differential plating, non-adhering cells were cultured in media supplemented with different concentrations of serum (0, 1 %, 2 %, 5 %, 10 %). After 10 days of primary culture, the cells were maintained in media supplemented with different concentrations of growth factors (basic fibroblast growth factor and epidermal growth factor at 1, 5, 10 ng/ml). The number of MGSC-derived colonies with different sizes was determined in each treatment to assess the effects of serum concentrations and growth factors. Results The number of MGSC-derived colonies was significantly higher in the presence of 1 % rather than 10 % fetal bovine serum (FBS). Basic fibroblast growth factor (bFGF) at 1, 5 ng/ml and epidermal growth factor (EGF) at 5, 10-ng/ml significantly promoted colony formation. Immunocytochemistry, reverse transcriptase-polymerase chain reaction (RT-PCR) and xenotransplantation assays demonstrated the presence of functional stem cells in cultured cell population.Conclusions In vitro propagation of porcine MGSCs could be maintained in the presence of 1 % FBS and supplementation of growth factors for 1 month.

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“…compared with other animal models [31]. However, few attempts have been done to develop porcine MGSC culture in vitro.…”

Section: Introductionmentioning

confidence: 99%

In vitro propagation of male germline stem cells from piglets

Zheng

Tian

Zhang

et al. 2013

J Assist Reprod Genet

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“…Recently, the fidelity of inflammatory/immune responses in the mouse model to replicate that of humans has been challenged (71). As an alternative, the pig is emerging as an important nonprimate biomedical model for human research due to its similar genetics, metabolic features, cardiovascular system, proportional organ sizes, and pathophysiology (21,82). In particular, the Ossabaw pig has a number of advantages among porcine models available for the study of obesity-associated metabolic and cardiovascular diseases (25,57).…”

mentioning

confidence: 99%

Vascular transcriptional alterations produced by juvenile obesity in Ossabaw swine

Padilla

Jenkins

Lee

et al. 2013

Physiological Genomics

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We adopted a transcriptome-wide microarray analysis approach to determine the extent to which vascular gene expression is altered as a result of juvenile obesity and identify obesity-responsive mRNAs. We examined transcriptional profiles in the left anterior descending coronary artery (LAD), perivascular fat adjacent to the LAD, and descending thoracic aorta between obese ( n = 5) and lean ( n = 6) juvenile Ossabaw pigs (age = 22 wk). Obesity was experimentally induced by feeding the animals a high-fat/high-fructose corn syrup/high-cholesterol diet for 16 wk. We found that expression of 189 vascular cell genes in the LAD and expression of 165 genes in the thoracic aorta were altered with juvenile obesity (false discovery rate ≤ 10%) with an overlap of only 28 genes between both arteries. Notably, a number of genes found to be markedly upregulated in the LAD of obese pigs are implicated in atherosclerosis, including ACP5, LYZ, CXCL14, APOE, PLA2G7, LGALS3, SPP1, ITGB2, CYBB, and P2RY12. Furthermore, pathway analysis revealed the induction of proinflammatory and pro-oxidant pathways with obesity primarily in the LAD. Gene expression in the LAD perivascular fat was minimally altered with juvenile obesity. Together, we provide new evidence that obesity produces artery-specific changes in pretranslational regulation with a clear upregulation of proatherogenic genes in the LAD. Our data may offer potential viable drug targets and mechanistic insights regarding the molecular precursors involved in the origins of overnutrition and obesity-associated vascular disease. In particular, our results suggest that the oxidized LDL/LOX-1/NF-κB signaling axis may be involved in the early initiation of a juvenile obesity-induced proatherogenic coronary artery phenotype.

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“…Surgical and anesthetic procedures are well established in the pig [18], and the genome is today fully sequenced in parallel with the existence of an important homology between the human and pig genome [19]. However, regarding the gestation, the pig has some important differences from the human pregnancy.…”

Section: Pigsmentioning

confidence: 99%

Animal Models of Fetal Medicine and Obstetrics

Andersen¹,

Alstrup²,

Duvald³

et al. 2018

Experimental Animal Models of Human Diseases - An Effective Therapeutic Strategy

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Animal models remain essential to understand the fundamental mechanisms occurring in fetal medicine and obstetric diseases, such as intrauterine growth restriction, preeclampsia and gestational diabetes. These vary regarding the employed method used for induction of the disease, and differ in relation to the animal characteristics (size, number of fetuses, placenta barrier type, etc.). While none of these exactly mirrors the human condition, different pregnant animal models (mice, rats, guinea pigs, chinchillas, rabbits, sheep and pigs) are here described with respect to advantages and limitations. The ability to employ noninvasively diagnostics varies among species, specifically for ultrasound and clinical magnetic resonance imaging procedures. Management of feeding, handling, care and anesthesia are particularly important factors in the pregnant animal.

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Completion of the swine genome will simplify the production of swine as a large animal biomedical model

Cited by 100 publications

References 111 publications

In vitro propagation of male germline stem cells from piglets

In vitro propagation of male germline stem cells from piglets

Vascular transcriptional alterations produced by juvenile obesity in Ossabaw swine

Animal Models of Fetal Medicine and Obstetrics

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