Animal Models of Fetal Medicine and Obstetrics

Andersen, Morten Nørgaard; Alstrup, Aage Kristian Olsen; Duvald, ChristinaSøndergaard; Mikkelsen, E.; Vendelbo, Mikkel Holm; Ovesen, Per; Pedersen, Michael

doi:10.5772/intechopen.74038

Cited by 23 publications

(20 citation statements)

References 105 publications

(81 reference statements)

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“…1 Master regulators are molecules that indirectly control multiple genes in a pathway within the ingenuity pathway analysis. 2 Molecule type of the regulator as defined by the ingenuity pathway analysis. 3 Network bias corrected p-value calculated by ingenuity pathway analysis.…”

Section: Ingenuity Pathway Analysismentioning

confidence: 99%

“…The limited availability of human samples has made it difficult to study the etiology of SA and reduce SA in humans. Mice are commonly used to study human gestation research due to low costs, a short 20 days gestation, and genetic engineering [2]. However, there are limitations in using mice as a model for human pregnancy.…”

Section: Introductionmentioning

confidence: 99%

“…However, there are limitations in using mice as a model for human pregnancy. Mice are a litter bearing species, and their significantly shortened gestation period makes it difficult to measure individual fetal progress and placental development needed for human studies [2]. These disparities encourage the use of models other than murine species to study the etiology and genetic factors that contribute to SA.…”

Section: Introductionmentioning

confidence: 99%

“…Database source; GO = Gene Ontology, KEGG = Kyoto Encyclopedia of Genes and Genomes, and R = Reactome 2. Number of genes associated with SA 3.…”

mentioning

confidence: 99%

“…Name of canonical pathways identified by ingenuity pathway analysis for fetal loss in Holstein cattle 2. Significance expressed as a Benjamini-Hochberg (B-H) corrected p-value for an association with spontaneous abortion 3.…”

mentioning

confidence: 99%

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Genomic Analysis of Spontaneous Abortion in Holstein Heifers and Primiparous Cows

Oliver

Wahl

Dick

et al. 2019

Genes

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Background: The objectives of this study were to identify loci, positional candidate genes, gene-sets, and pathways associated with spontaneous abortion (SA) in cattle and compare these results with previous human SA studies to determine if cattle are a good SA model for humans. Pregnancy was determined at gestation day 35 for Holstein heifers and cows. Genotypes from 43,984 SNPs of 499 pregnant heifers and 498 pregnant cows that calved at full term (FT) were compared to 62 heifers and 28 cows experiencing SA. A genome-wide association analysis, gene-set enrichment analysis-single nucleotide polymorphism, and ingenuity pathway analysis were used to identify regions, pathways, and master regulators associated with SA in heifers, cows, and a combined population. Results: Twenty-three loci and 21 positional candidate genes were associated (p < 1 × 10 −5 ) with SA and one of these (KIR3DS1) has been associated with SA in humans. Eight gene-sets (NES > 3.0) were enriched in SA and one was previously reported as enriched in human SA. Four master regulators (p < 0.01) were associated with SA within two populations. Conclusions: One locus associated with SA was validated and 39 positional candidate and leading-edge genes and 2 gene-sets were enriched in SA in cattle and in humans.

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Section: Ingenuity Pathway Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Database source; GO = Gene Ontology, KEGG = Kyoto Encyclopedia of Genes and Genomes, and R = Reactome 2. Number of genes associated with SA 3.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

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Genomic Analysis of Spontaneous Abortion in Holstein Heifers and Primiparous Cows

Oliver

Wahl

Dick

et al. 2019

Genes

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Engineered Platelet‐Derived Growth Factor‐Releasing Hydrogels Promote Fetal Membrane Healing In Vivo

Avilla-Royo

Vonzun

Seehusen

et al. 2023

Adv Funct Materials

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Fetoscopic interventions to treat fetal anomalies are currently performed for a variety of conditions. Depending on the procedure, preterm rupture of the fetal membranes (FMs) happens in around 30% of the cases, potentially leading to preterm birth and fetal morbidity and mortality. Here, the capacity of modular transglutaminase crosslinked poly(ethylene glycol) (TG-PEG) hydrogels that release platelet-derived growth factor (PDGF)-BB to promote FM healing is described. In vitro, such growth factor-loaded hydrogels are able to stimulate amniotic cell migration and proliferation. When applied in vivo, these TG-PEG hydrogels tightly seal the FM and uterus defects created by a fetoscope and remain stable for 10 days. The migration of healing-related cells into such hydrogels in the myometrium, endometrium, and FM areas is only possible in soft TG-PEG hydrogels. Importantly, bioengineered hydrogels releasing PDGF-BB promote recruitment of host cells from the myometrium and the endometrium, and to a lesser extent from FM areas. In such hydrogels, the potent proliferation and matrix production of the recruited cells at the site of treatment into the biomaterial initiates a robust early healing response. PDGF-BB-loaded TG-PEG hydrogels hold great promise for the treatment of fetoscopy-induced FM defects and for the prevention of preterm birth.

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Interface‐Stabilized Fiber Sensor for Real‐Time Monitoring of Amniotic Fluid During Pregnancy

Li,

et al. 2023

Advanced Materials

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Diseases in pregnancy endanger millions of fetuses worldwide every year. The onset of these diseases can be early warned by the dynamic abnormalities of biochemicals in amniotic fluid, thus requiring real‐time monitoring. However, when continuously penetrated by detection devices, the amnion is prone to loss of robustness and rupture, which is difficult to regenerate. Here, we present an interface‐stabilized fiber sensor for real‐time monitoring of biochemical dynamics in amniotic fluid during pregnancy. The sensor is seamlessly integrated into the amnion through tissue adhesion, amniotic regeneration and uniform stress distribution, posing no risk to the amniotic fluid environment. The sensor demonstrates a response performance of less than 0.3% fluctuation under complex dynamic conditions and an accuracy of more than 98% from the second to the third trimester. By applying it to early warning of diseases such as intrauterine hypoxia, intrauterine infection, and fetal growth restriction, fetal survival increases to 95% with timely intervention.This article is protected by copyright. All rights reserved

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Animal Models of Fetal Medicine and Obstetrics

Cited by 23 publications

References 105 publications

Genomic Analysis of Spontaneous Abortion in Holstein Heifers and Primiparous Cows

Genomic Analysis of Spontaneous Abortion in Holstein Heifers and Primiparous Cows

Engineered Platelet‐Derived Growth Factor‐Releasing Hydrogels Promote Fetal Membrane Healing In Vivo

Interface‐Stabilized Fiber Sensor for Real‐Time Monitoring of Amniotic Fluid During Pregnancy

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