Complete sequencing of the FII plasmid pHK01, encoding CTX-M-14, and molecular analysis of its variants among Escherichia coli from Hong Kong

Ho, Pak‐Leung; Lo, Wai-U; Wong, River Chun-Wai; Yeung, Man Kiu; Chow, Kin-Hung; Que, Tak-Lun; Tong, Amy; Bao, Jessie Y.J.; Lok, Si; Wong, Samson Sai-Yin

doi:10.1093/jac/dkr010

Cited by 28 publications

(32 citation statements)

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“…J53 transconjugants carrying all the targets in two completely sequenced plasmids (pHK01 and pHK23a) and the J53 recipient strain were used as positive and negative controls, respectively (Ho et al . , ).…”

Section: Methodsmentioning

confidence: 99%

Dissemination of plasmid-mediated fosfomycin resistance fosA3 among multidrug-resistant Escherichia coli from livestock and other animals

Chan

et al. 2012

J Appl Microbiol

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Aims: To investigate plasmid-mediated fosfomycin resistance related to fosA3 in Escherichia coli isolates collected from different animals in Hong Kong, China, 2008. Methods and Results: In total, 2106 faecal specimens from 210 cattle, 214 pigs, 460 chickens, 398 stray cats, 368 stray dogs and 456 wild rodents were cultured. The faecal colonization rates of fosfomycin-resistant E. coli were as follows: 11Á2% in pigs, 8Á6% in cattle, 7Á3% in chickens, 2Á4% in dogs, 0Á8% in cats and 1Á5% in rodents. The cultures yielded 1693 isolates of which 831 were extended-spectrum b-lactamases (ESBL) producers. Fosfomycin-resistant isolates were more likely than fosfomycin-susceptible isolates to be producers of ESBL and to have resistance to chloramphenicol, ciprofloxacin, cotrimoxazole, gentamicin and tetracycline. Of the 101 fosfomycin-resistant isolates, 97 (96Á0%) isolates were fosA3 positive and 94 (93Á1%) were bla CTX-M positive. PCR mapping showed that the fosA3-containing regions were flanked by IS26, both upstream and downstream in 81 (83Á5%) isolates, and by an upstream bla CTX-M-14 -containing transposon-like structure (DISEcp1-bla CTX-M-14 -DIS903

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Section: Methodsmentioning

confidence: 99%

Dissemination of plasmid-mediated fosfomycin resistance fosA3 among multidrug-resistant Escherichia coli from livestock and other animals

Chan

et al. 2012

J Appl Microbiol

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“…It is suggested that this F2:AϪ:BϪ plasmid is a rather stable plasmid circulating in different members of Enterobacteriaceae and is present in different animal and human reservoirs in China. The F2:AϪ:BϪ plasmids are also associated with bla CTX-M genes in Enterobacteriaceae isolates from China, Hong Kong, South Korea, Vietnam, Italy, Canada, the United Kingdom, and Belgium (12,14,20,24,26,30,34). Further studies are needed to address the mechanisms underlying the worldwide dissemination of these plasmid types.…”

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confidence: 99%

F33:A−:B− and F2:A−:B− Plasmids Mediate Dissemination of rmtB-bla _CTX-M-9 Group Genes and rmtB-qepA in Enterobacteriaceae Isolates from Pets in China

Deng

Chen

et al. 2011

Antimicrob Agents Chemother

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This study investigated the prevalence of 16S rRNA methylase genes in 267 Enterobacteriaceae isolates collected from pets. The rmtB gene was detected in 69 isolates, most of which were clonally unrelated. The coexistence of the rmtB gene with the bla CTX-M-9 group genes and/or qepA within the same IncFII replicons was commonly detected. The two dominant types of IncF plasmids, F2:A؊:B؊, carrying rmtB-qepA, and F33:A؊: B؊, carrying the rmtB-bla CTX-M-9 group genes (and especially bla CTX-M-65 ), shared restriction patterns within each incompatibility group.Recently, the production of 16S rRNA methylases by Gramnegative bacilli has emerged as a novel mechanism for their high-level resistance to aminoglycosides (9, 31). Seven plasmid-mediated 16S rRNA methylase genes, rmtA, rmtB, rmtC, rmtD, rmtE, armA, and npmA, have been identified so far in multiple species of Enterobacteriaceae (3,6,9,13,31). These resistance determinants are globally disseminated, with rmtB and armA being the most frequently reported types worldwide (3,5,9,10,11,13,19,27,29,32). However, even though aminoglycosides are widely used in pets to treat Gram-negative bacterial infections, the prevalence of 16S rRNA methylases in bacteria from pets is not known. In addition, the 16S rRNA methylase genes, especially rmtB, are commonly associated with bla CTX-M genes (1-3, 8, 13, 19, 27, 29, 32). In our recent study on the distribution of extended-spectrum ␤-lactamase (ESBL) genes in Escherichia coli isolates obtained from pets, some CTX-M-producing isolates showed significantly reduced susceptibilities to amikacin, and this resistance to amikacin was cotransferred with CTX-M-9 subgroup genes to recipients (25). The current study investigated the prevalence of 16S rRNA methylase genes among CTX-M-producing isolates and characterized the plasmids carrying rmtB.The present study included 135 CTX-M-producing Enterobacteriaceae isolates (119 E. coli, 11 Klebsiella pneumoniae, 3 Enterobacter cloacae, and 2 Citrobacter freundii isolates) recovered from healthy or diseased pets (dogs and cats) in Guangdong, China, during 2006 and 2008. The ESBL genes in most of these strains have been characterized previously (18, 25).The gene type of bla CTX-M was confirmed by PCR and DNA sequencing (25). In addition, this study also included 132 Enterobacteriaceae isolates previously confirmed to be CTX-M negative that were collected from pets in Guangdong province of China during 2006 and 2008 (18, 25). The presence of 16S rRNA methylase genes was identified by PCR using previously designed primers (5, 6, 9). Of the 135 CTX-M producers, 60 (ϳ44%) were positive for rmtB and 5 (ϳ4%) were positive for armA (Table 1). No isolate was positive for the rmtA, rmtC, rmtD, rmtE, or npmA genes. The rmtB gene was also detected in 9 (ϳ7%) of the 132 CTX-M-negative isolates. Therefore, of the 69 rmtB-positive isolates, 60 were CTX-M producers, and most of the enzymes produced belonged to the CTX-M-9 group. Since the plasmid-mediated fluoroquinolone efflux pump gene qepA is frequently as...

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“…ST131 and ST405 are virulent clones that have been found globally among disease isolates from human (Banerjee et al, 2013;Ho et al, 2012b). STC10, STC38 and STC155 isolates have been commonly found as part of the normal flora in humans, pigs, cattle and poultry (Lo et al, 2010(Lo et al, , 2014Manges & Johnson, 2012), and to cause human urinary tract and bacteraemic infections (Ho et al, 2011b(Ho et al, , 2012a.…”

Section: Discussionmentioning

confidence: 99%

“…The bla CTX-M genes were detected by PCR using primers with specificity for the CTX-M subgroups (bla CTX-M1G, bla CTX-M2G, bla CTX-M8G, bla CTX-M9G and bla CTX-M25G ) (D'Andrea et al, 2013;Ho et al, 2011bHo et al, , 2012a. PCR assays were used to assign the E. coli isolates to phylogroups A, B1, B2, C, D, E and F (Clermont et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Clonal diversity of CTX-M-producing, multidrug-resistant Escherichia coli from rodents

Lai

et al. 2015

Journal of Medical Microbiology

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This territory-wide study investigated the occurrence of faecal carriage of extended-spectrum blactamase (ESBL)-producing Escherichia coli among wild rodents from the 18 districts in Hong Kong. Individual rectal swabs were obtained from the trapped animals and cultured in plain and selective media. A total of 965 wild rodents [148 chestnut spiny rats (Niviventer fulvescens), 326 Indo-Chinese forest rats (Rattus andamanensis), 452 brown rats (Rattus norvegicus) and 39 black rats (Rattus rattus)] were sampled. ESBL carriage was 0 % in chestnut spiny rats, 0.6 % in Indo-Chinese forest rats, 7.7 % in black rats and 13.9 % in brown rats. Among brown rats, the prevalence of ESBL carriage differed markedly by geographical location: absent in two districts, low (7-10 %) in six districts, moderate (11-19 %) in seven districts and high (21-50 %) in three districts. Nonetheless, there was no correlation between the prevalence of ESBL in brown rats and human population density in the 18 districts. CTX-M-type enzymes were detected in 92.0 % of the ESBL-producing isolates, of which 83.1 % were resistant to three or more non-b-lactam drugs. The CTX-M producing isolates were genetically diverse but a large proportion (47.8 %) were included in six successful clones that are strongly associated with human diseases and CTX-M dissemination, viz. sequence type complex [STC]10/phylogroup A, STC23/phylogroup B1, STC38/phylogroup D, STC155/phylogroup B1, ST405/phylogroup D and ST131/ phylogroup B2. In conclusion, our results show that brown rats often carry potentially zoonotic clones of CTX-M producing, multidrug-resistant E. coli. The potential for rats to be a source of CTX-M producing E. coli for humans deserves further consideration.

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Complete sequencing of the FII plasmid pHK01, encoding CTX-M-14, and molecular analysis of its variants among Escherichia coli from Hong Kong

Abstract: This study showed that narrow host-range FII plasmids play important roles in the dissemination of CTX-M-14. FII plasmids closely related to pHK01 have disseminated widely in the Hong Kong community.

Cited by 28 publications

References 9 publications

Dissemination of plasmid-mediated fosfomycin resistance fosA3 among multidrug-resistant Escherichia coli from livestock and other animals

Dissemination of plasmid-mediated fosfomycin resistance fosA3 among multidrug-resistant Escherichia coli from livestock and other animals

F33:A−:B− and F2:A−:B− Plasmids Mediate Dissemination of rmtB-bla _CTX-M-9 Group Genes and rmtB-qepA in Enterobacteriaceae Isolates from Pets in China

Clonal diversity of CTX-M-producing, multidrug-resistant Escherichia coli from rodents

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Complete sequencing of the FII plasmid pHK01, encoding CTX-M-14, and molecular analysis of its variants among Escherichia coli from Hong Kong

Abstract: This study showed that narrow host-range FII plasmids play important roles in the dissemination of CTX-M-14. FII plasmids closely related to pHK01 have disseminated widely in the Hong Kong community.

Cited by 28 publications

References 9 publications

Dissemination of plasmid-mediated fosfomycin resistance fosA3 among multidrug-resistant Escherichia coli from livestock and other animals

Dissemination of plasmid-mediated fosfomycin resistance fosA3 among multidrug-resistant Escherichia coli from livestock and other animals

F33:A−:B− and F2:A−:B− Plasmids Mediate Dissemination of rmtB-bla CTX-M-9 Group Genes and rmtB-qepA in Enterobacteriaceae Isolates from Pets in China

Clonal diversity of CTX-M-producing, multidrug-resistant Escherichia coli from rodents

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F33:A−:B− and F2:A−:B− Plasmids Mediate Dissemination of rmtB-bla _CTX-M-9 Group Genes and rmtB-qepA in Enterobacteriaceae Isolates from Pets in China