Complete sequence of a type-I microfibrillar wool keratin gene

Application of proteomics for determining protein markers for wool quality traits

“…Using mass spectral (MS) peptide mapping the highest correlation for spot 1 (Fig. 5 and Table 2) was found to match with sheep keratin Type I microfibrillar protein K1M2 [21]. Twenty-three peptides from the MS peptide map of this spot were matched with masses from the sequence of sheep K1M2 and these provided a 67% coverage of the protein sequence (Fig.…”

Problems Associated with the Identification of Proteins in Homologous Families: The Wool Keratin Family as a Case Study

“…The IFPs can be further divided into two families: the Type I and Type II, each family containing four proteins (1). It is the interaction of one Type I and one Type II IFP that results in the formation of a dimer, the basic building block of the intermediate filament (2). Sixteen dimers are present in transverse cross-sections of each intermediate filament (3).…”

“…Keratin proteins are the main structural components of wool and can be divided into two groups, the intermediate filament proteins (IFPs) 2 and the matrix proteins. The IFPs can be further divided into two families: the Type I and Type II, each family containing four proteins (1).…”

Electrophoretic mapping of highly homologous keratins: A novel marker peptide approach

“…The IFP complement of wool is less clear. The existence of only four Type I and four Type II IFPs is known [4], and of these, only two Type Is have been sequenced: ovine K31 [5] and K33a [6], and three Type IIs: ovine K81 [7], K83 [8] and K85 [9]. Historically, this has resulted in the assignment of the four strings of spots associated with the wool Type I IFPs in a 2-DE map, to the two known proteins in sequence databases [10].…”