Application of proteomics for determining protein markers for wool quality traits

Plowman, Jeffrey E.; Bryson, Warren G.; Jordan, T. William

doi:10.1002/(sici)1522-2683(20000501)21:9<1899::aid-elps1899>3.0.co;2-r

Cited by 37 publications

(14 citation statements)

References 18 publications

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“…Wool fiber characteristics, such as diameter, crimps, and length, are essential parameters of the wool trait, as well as important indications of the spinning efficiency of the wool (Plowman et al, 2009). Previous studies have shown that variations in the protein compositions of the orthocortex and paracortex, which make up nearly the whole wool fiber, were highly related with wool trait parameters (Marshall et al, 1991;Plowman et al, 2000). Particularly, the formation of KAP composites gives the wool special mechanical attributes of strength, inertness, and rigidity (Parry and Steinert, 1992;Plowman, 2003;Plowman et al, 2009).…”

Section: Introductionsupporting

confidence: 82%

Polymorphisms of KAP6, KAP7, and KAP8 genes in four Chinese sheep breeds

et al. 2014

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ABSTRACT. High glycine-tyrosine proteins (HGTPs), also known as keratin-associated proteins (KAPs), play a key role in the major structures and mechanical properties of wool fiber. Sheep HGTPs consist of three multigene families: KAP6, KAP7, and KAP8 genes. Polymorphisms of these three genes have been proposed to have important effects on wool fiber traits. The aim of the present study was to identify polymorphisms of the KAP6, KAP7, and KAP8 genes in four sheep breeds, including Chinese Merino superfine wool sheep, Hu sheep, a Merino x Hu crossed breed, and Romney sheep. Polymerase chain reaction (PCR) product direct sequencing, PCR-single-strand conformation polymorphism, and cloned sequencing methods were used to find genetic variation and identify polymorphisms in these genes. The Mutation Surveyor v3.97 software was used to analyze the sequences. These methods revealed six different sequences of the KAP6 gene, two different sequences of the KAP7 gene, and five different sequences of the KAP8 gene. Accordingly, 3439©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (2): 3438-3445 (2014) Polymorphisms of sheep wool keratin-associated protein genes three (with frequencies >1%) single nucleotide polymorphisms (SNPs) of the KAP6 gene, one SNP of the KAP7 gene, and five SNPs of the KAP8 gene were detected. Interestingly, some of these sequences were present in only certain sheep breeds, thereby suggesting that these special allele sequences could be used as candidate genes of wool characteristics in further studies.

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Section: Introductionsupporting

confidence: 82%

Polymorphisms of KAP6, KAP7, and KAP8 genes in four Chinese sheep breeds

et al. 2014

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“…CBB G) proved to give higher sensitivity than the silver patterns stained in parallel. Those gave negatively stained spots for intermediate filament proteins, the major high sulfur proteins and the high glycine tyrosine proteins [193]. Lopez et al [68] found about 90% similar proteins in silver-and SYPRO Ruby stained rat fibroblast cell lysates, whereas Chevallet et al [55] showed differential images obtained with acidic, ammoniacal and the Shevchenko silver stains, and RuBP, for human erythrocyte membrane and mouse macrophage cell line proteins.…”

Section: Examples From Literature (For Comparison Of Different Dyes)mentioning

confidence: 99%

Protein stains for proteomic applications:Which, when, why?

Crawford²,

2006

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This review recollects literature data on sensitivity and dynamic range for the most commonly used colorimetric and fluorescent dyes for general protein staining, and summarizes procedures for the most common PTM-specific detection methods. It also compiles some important points to be considered in imaging and evaluation. In addition to theoretical considerations, examples are provided to illustrate differential staining of specific proteins with different detection methods. This includes a large body of original data on the comparative evaluation of several pre-and post-electrophoresis stains used in parallel on a single specimen, horse serum run in 2-DE (IPG-DALT). A number of proteins/protein spots are found to be over-or under-revealed with some of the staining procedures.

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“…A major part of them can subsequently be analyzed using classical techniques. Separation by electrophoresis41 and isoelectric focusing was successfully used to identify species‐specific keratins,37, 40 and sequencing data from many different types of keratins have been published 25, 37…”

Section: The Tyrolean Mummymentioning

confidence: 99%

Species identification of Oetzi's clothing with matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry based on peptide pattern similarities of hair digests

Hollemeyer

Altmeyer²,

Heinzle

et al. 2008

Rapid Comm Mass Spectrometry

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Identification of ancient biological samples from the 1991-discovered and more than 5300-year-old Tyrolean mummy, also called iceman or Oetzi, is very difficult. The species of origins of four animal-hair-bearing samples of the accoutrement of the mummy not yet diagnosed were identified by a special proteomics method. Ha 43/91/130 and Ha 6/91, two samples from his coat, and Ha 5/91, a sample from his leggings, were assigned to sheep. The upper leather of his moccasins, Ha 2/91, was made from cattle. Despite the enormous age of these samples with partial (bio)chemical alterations, reliable identification was possible using a recently developed matrix-assisted laser desorption/ionization time-of-flight mass spectrometric ((MALDI-TOF MS)-based analytical method. The method is exclusively based on the analysis of proteins and uses minute amounts of peptides directly derived from tryptic hair digests without any separation or enrichment steps. Unknown species are identified by comparison of their peptide ion patterns with known spectra stored in existing databases. Hereby, the correlation distance, a form of Euclidean distance, and deduced parameters are used to measure similarities. If more than one potential hit remains, specific diagnostic peptide ions are used to stepwise exclude incorrect matches. These ions are specific for orders, families, subfamilies/genera and/or even species. Peptide mass fingerprinting data combined with those from collision-induced dissociation spectra (combined MS & MS/MS) were used for interpretation with the MASCOT search engine and the NCBI database to find the potential parentage of hair proteins. For this technique, selected precursor ions were identified as specific diagnostic peptide ions.

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Application of proteomics for determining protein markers for wool quality traits

Cited by 37 publications

References 18 publications

Polymorphisms of KAP6, KAP7, and KAP8 genes in four Chinese sheep breeds

Polymorphisms of KAP6, KAP7, and KAP8 genes in four Chinese sheep breeds

Protein stains for proteomic applications:Which, when, why?

Species identification of Oetzi's clothing with matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry based on peptide pattern similarities of hair digests

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