Complete Oxidation of Methanol in Biobattery Devices Using a Hydrogel Created from Three Modified Dehydrogenases

Kim, Yang‐Hee; Campbell, Elliot; Yu, Jiang; Minteer, Shelley D.; Banta, Scott

doi:10.1002/ange.201207423

Cited by 27 publications

(27 citation statements)

References 30 publications

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“…Maltodextrin is slowly utilized via the synthetic pathway to generate a nearly constant power output (Fig. 3) rather than a peak power over a short time 10 . In addition, most enzymes cannot work well in high concentrations of alcohol or glucose due to inhibition or low water activity.…”

Section: Discussionmentioning

confidence: 99%

“…The incomplete oxidation of organic fuels mediated by one or a few enzymes in EFCs results in low energy densities, waste of fuel and inhibition of enzymes by the metabolic products. In vitro synthetic enzymatic pathways have been constructed on the anode compartments in EFCs for deep or complete oxidation of methanol 9,10 , ethanol 11 , glycerol 12 and glucose 13 . However, previous studies did not provide quantitative evidence (for example, Faraday efficiency) for the complete oxidation of organic fuels in microbial fuel cells 14,15 .…”

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confidence: 99%

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A high-energy-density sugar biobattery based on a synthetic enzymatic pathway

Zhu

Tam²,

Sun³

et al. 2014

Nat Commun

247

194

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High-energy-density, green, safe batteries are highly desirable for meeting the rapidly growing needs of portable electronics. The incomplete oxidation of sugars mediated by one or a few enzymes in enzymatic fuel cells suffers from low energy densities and slow reaction rates. Here we show that nearly 24 electrons per glucose unit of maltodextrin can be produced through a synthetic catabolic pathway that comprises 13 enzymes in an air-breathing enzymatic fuel cell. This enzymatic fuel cell is based on non-immobilized enzymes that exhibit a maximum power output of 0.8 mW cm À 2 and a maximum current density of 6 mA cm À 2 , which are far higher than the values for systems based on immobilized enzymes. Enzymatic fuel cells containing a 15% (wt/v) maltodextrin solution have an energy-storage density of 596 Ah kg À 1 , which is one order of magnitude higher than that of lithium-ion batteries. Sugar-powered biobatteries could serve as next-generation green power sources, particularly for portable electronics.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

A high-energy-density sugar biobattery based on a synthetic enzymatic pathway

Zhu

Tam²,

Sun³

et al. 2014

Nat Commun

247

194

View full text Add to dashboard Cite

show abstract

“…Similarly, in some of the studies multi enzyme cascade was used for the complete oxidation of the substrates such as glucose [293], methanol [294], ethanol [295], pyruvate [296][297][298], glycerol [299][300][301], etc., to CO 2 generating higher number of electrons. Modified electrodes combining cellobiose dehydrogenase and pyranose dehydrogenase have shown to be capable of extracting up to 6 electrons from one molecule of glucose [302].…”

Section: Multi Enzyme Cascadesmentioning

confidence: 99%

Enzymatic Electrosynthesis: An Overview on the Progress in Enzyme- Electrodes for the Production of Electricity, Fuels and Chemicals

Satyawali¹

2013

J Microbial Biochem Technol

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Recent interest in the field of biocommodities production through bioelectrochemical systems has generated interest in the enzyme catalyzed redox reactions. Enzyme catalyzed electrodes are well established as sensors and power generators. However, a paradigm shift in recent science towards the production of useful chemicals has changed the face of biofuel cells, keeping the fuels or chemicals production in the upfront. This review article comprehensively presents the progress in the field of enzyme-electrodes for the production of electricity, fuels and chemicals with an aim to represent a practical outline for understanding the use of single or multiple redox enzymes as electrocatalysts for their electron transfer onto electrodes. It also provides the state-of-the-art information regarding the different existing processes to fabricate enzyme electrodes. Successfully-achieved electroenzymatic anodic and cathodic reactions are further discussed, together with their potential applications. Particular focus was made on the novel single/multiple enzyme systems towards product synthesis and other applications. Finally, techno-economic and environmental elements for industrial processing with enzyme catalyzed bioelectrochemical system (e-BES) are anticipated, in order to provide useful strategies for further development of this technology.

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“…The system was recently extended to support a synthetic metabolic pathway involving a cascade of three NAD(H)-dependent dehydrogenase enzymes. 16 This system consisted of three components, two tetrameric enzymes and one dimeric, each expressed with a physical crosslinking functionality facilitated through an H and S domain. When the enzymes were incorporated together in this system, they facilitated the complete oxidation of methanol to carbon dioxide in a biobattery.…”

Section: Self-assembling Protein Systemsmentioning

confidence: 99%

The use of engineered protein materials in electrochemical devices

Renner

2016

Exp Biol Med (Maywood)

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Bioelectrochemical technologies have an important and growing role in healthcare, with applications in sensing and diagnostics, as well as the potential to be used as implantable power sources and be integrated with automated drug delivery systems. Challenges associated with enzyme-based electrodes include low current density and short functional lifetimes. Protein engineering is emerging as a powerful tool to overcome these issues. By taking advantage of the ability to precisely define protein sequences, electrodes can be organized into high performing structures, and enable the next generation of medical devices.

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Complete Oxidation of Methanol in Biobattery Devices Using a Hydrogel Created from Three Modified Dehydrogenases

Cited by 27 publications

References 30 publications

A high-energy-density sugar biobattery based on a synthetic enzymatic pathway

A high-energy-density sugar biobattery based on a synthetic enzymatic pathway

Enzymatic Electrosynthesis: An Overview on the Progress in Enzyme- Electrodes for the Production of Electricity, Fuels and Chemicals

The use of engineered protein materials in electrochemical devices

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