Complete nucleotide sequences of Bacillus plasmids pUB110dB, pRBH1 and its copy mutants

Müller, Robert; Ano, Takashi; Imanaka, Tadayuki; Aiba, Setsuya

doi:10.1007/bf00330534

Cited by 47 publications

(26 citation statements)

References 17 publications

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“…2), and we have demonstrated that pIM13 and pE5 accumulate single-stranded DNA. We also noted that there was some homology between the pE5 deletion site, a site in pUB110-pC194 cointegrates that is a recombinational hot spot (5), and a sequence on pTB19 (a Bacillus stearothermophilus plasmid) which is at ajunction between regions of homology and nonhomology with pUB110 (13). These potential intraplasmid recombination sites are shown in Fig.…”

Section: Methodsmentioning

confidence: 82%

Replication properties of pIM13, a naturally occurring plasmid found in Bacillus subtilis, and of its close relative pE5, a plasmid native to Staphylococcus aureus

Projan¹,

Monod²,

Narayanan³

et al. 1987

J Bacteriol

100

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A naturally occurring plasmid from Bacillus subtilis, pIM13, codes for constitutively expressed macrolidelincosamide-streptogramin B (MLS) resistance, is stably maintained at a high copy number, and exists as a series of covalent multimers. The complete sequence of pIM13 has been reported (M. Monod, C. Denoya, and D. Dubnau, J. Bacteriol. 167:138-147, 1986) and two long open reading frames have been identified, one of which (ermC') is greater than 90% homologous to the ermC MLS resistance determinant of the Staphylococcus aureus plasmid pE194. The second reading frame (repL) shares homology with the only long open reading frame of the cryptic S. aureus plasmid pSN2 and is probably involved in plasmid replication. The map of pIM13 is almost a precise match with that of pE5, a naturally occurring, stable, low-copy-number, inducible MLS resistance plasmid found in S. aureus. pIM13 is unstable in S. aureus but still multimerizes in that host, while pE5 is unstable in B. subtilis and does not form multimers in either host. The complete sequence of pE5 is presented, and comparison between pIM13 and pE5 revealed two stretches of sequence present in pE5 that were missing from pIM13. It is likely that a 107-base-pair segment in the ermC' leader region missing from pIM13 accounts for the constitutive nature of the pIM13 MLS resistance and that the lack of an additional 120-base-pair segment in pIM13 that is present on pE5 gives rise to the high copy number, stability, and multimerization in B. subtilis. The missing 120 base pairs occur at the carboxy-terminal end of the putative replication protein coding sequence and results in truncation of that protein. It is suggested either that the missing segment contains a site involved in resolution of multimers into monomers or that the smaller replication protein causes defective termination of replication. It is concluded that pIM13 and pE5 are coancestral plasmids and it is probable that pIM13 arose from pE5.

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Section: Methodsmentioning

confidence: 82%

Replication properties of pIM13, a naturally occurring plasmid found in Bacillus subtilis, and of its close relative pE5, a plasmid native to Staphylococcus aureus

Projan¹,

Monod²,

Narayanan³

et al. 1987

J Bacteriol

100

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“…V01282) of plasmid pUB110 from S. aureus ( Fig. 1) (20,27). As previously mentioned, a gene that is identical, except for a 1-bp Table 1).…”

Section: Resultsmentioning

confidence: 99%

Chromosomal aadD2 Encodes an Aminoglycoside Nucleotidyltransferase in Bacillus clausii

Bozdoğan

Galopin

Gerbaud

et al. 2003

Antimicrob Agents Chemother

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Bacillus clausii SIN is one of the four strains of B. clausii composing a probiotic administered to humans for the prevention of gastrointestinal side effects due to oral antibiotic therapy. The strain is resistant to kanamycin, tobramycin, and amikacin. A gene conferring aminoglycoside resistance was cloned into Escherichia coli and sequenced. The gene, called aadD2, encoding a putative 246-amino acid protein, shared 47% identity with ant(4)-Ia from Staphylococcus aureus, which encodes an aminoglycoside 4-O-nucleotidyltransferase. Phosphocellulose paper-binding assays indicated that the gene product was responsible for nucleotidylation of kanamycin, tobramycin, and amikacin. The aadD2 gene was detected by DNA-DNA hybridization in the three other strains of the probiotic mixture and in the reference strain B. clausii DSM8716, although it did not confer resistance in these strains. Mutations in the sequence of the putative promoter for aadD2 from B. clausii SIN resulted in higher identity with consensus promoter sequences and may account for aminoglycoside resistance in that strain. The aadD2 gene was chromosomally located in all strains and was not transferable by conjugation. These data indicate that chromosomal aadD2 is specific to B. clausii.

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“…The ant(4Ј)-Ia genes (often referred to in the literature as aadD) were cloned from the plasmids of both Staphylococcus aureus and thermophilic bacilli and turned out to be almost identical except for one base pair difference (180,203). Recently, this gene was detected on the large conjugative plasmid from Staphylococcus aureus (20) and as a part of the mec region from methicillin-resistant staphylococci (138,277).…”

Section: Aminoglycoside Nucleotidyltransferasesmentioning

confidence: 99%

Versatility of Aminoglycosides and Prospects for Their Future

2003

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Aminoglycoside antibiotics have had a major impact on our ability to treat bacterial infections for the past half century. Whereas the interest in these versatile antibiotics continues to be high, their clinical utility has been compromised by widespread instances of resistance. The multitude of mechanisms of resistance is disconcerting but also illuminates how nature can manifest resistance when bacteria are confronted by antibiotics. This article reviews the most recent knowledge about the mechanisms of aminoglycoside action and the mechanisms of resistance to these antibiotics

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Complete nucleotide sequences of Bacillus plasmids pUB110dB, pRBH1 and its copy mutants

Cited by 47 publications

References 17 publications

Replication properties of pIM13, a naturally occurring plasmid found in Bacillus subtilis, and of its close relative pE5, a plasmid native to Staphylococcus aureus

Replication properties of pIM13, a naturally occurring plasmid found in Bacillus subtilis, and of its close relative pE5, a plasmid native to Staphylococcus aureus

Chromosomal aadD2 Encodes an Aminoglycoside Nucleotidyltransferase in Bacillus clausii

Versatility of Aminoglycosides and Prospects for Their Future

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