Complete nucleotide sequence of the mRNA coding for the N protein of vesicular stomatitis virus (New Jersey serotype)

Banerjee, Amiya K.; Rhodes, Dennis P.; Gill, Dalip S.

doi:10.1016/0042-6822(84)90237-x

Cited by 46 publications

(26 citation statements)

References 12 publications

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“…3b) indicated a clear similarity between the two proteins. The conservation of the NS proteins is thus considerably lower than that of the G proteins (51 ~o) or the N proteins (69 ~o) of the two serotypes (Gallione & Rose, 1983;Banerjee et al, 1984). There are 27 amino acid differences between the Missouri and Ogden strains (Fig.…”

Section: Comparison Of Vsv Ns Genes and Proteinsmentioning

confidence: 99%

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Conservation of Potential Phosphorylation Sites in the NS Proteins of the New Jersey and Indiana Serotypes of Vesicular Stomatitis Virus

Rae¹,

Elliott²

1986

Journal of General Virology

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SUMMARYA full length cDNA copy of the NS mRNA of the Missouri strain (Hazelhurst subtype, New Jersey serotype) of vesicular stomatitis virus (VSV) has been cloned and sequenced. The mRNA is 856 nucleotides long (excluding polyadenylic acid) and encodes a protein of 274 amino acids (mol. wt. 31000). Comparison with the NS gene of the Ogden strain (Concan subtype, New Jersey serotype) showed 15 ~ difference at the nucleotide level and 10Y/oo difference at the amino acid level; the majority of the changes were located in the Y half of the mRNA. Comparison with the NS genes of two strains representing the Indiana serotype showed about 50~ nucleotide and 33 ~ amino acid sequence homology between the serotypes. In a four-way comparison of the proteins, two regions of higher homology were noted which may be of functional importance. Eighteen potential phosphorylation sites (Ser or Thr) were conserved between the four proteins; five of these sites correspond to the residues which have been suggested to be constitutively phosphorylated and may be essential for NS activity.

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Section: Comparison Of Vsv Ns Genes and Proteinsmentioning

confidence: 99%

“…This shows that there are only short regions of significant homology scattered throughout the gene sequences. For comparison the nucleotide sequences of the G and N genes of the NJ and IND serotypes show 54~ and 68~ homology respectively (Gallione & Rose, 1983;Banerjee et al, 1984).…”

Section: Comparison Of Vsv Ns Genes and Proteinsmentioning

confidence: 99%

Conservation of Potential Phosphorylation Sites in the NS Proteins of the New Jersey and Indiana Serotypes of Vesicular Stomatitis Virus

Rae¹,

Elliott²

1986

Journal of General Virology

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“…2). Both rabies (19) and the New Jersey serotype (17) shared only three tetrapeptides and no pentapeptides with Ro/SSA, while Chandipura (18) and Piry strains (20), respectively, shared four and one tetrapeptides. Two of the New Jersey serotype N protein sequences overlap with central antigenic peptide, while only one overlaps in each of the Chandipura and rabies N proteins (Fig.…”

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confidence: 99%

“…Over 70 strains of rhabdoviruses are known, however, to infect vertebrates, and probably hundreds remain to be described (34,35). So there is a reasonable possibility that a rhabdovirus exists which would be an even better explanation for the autoantigenicity of Ro/SSA than are those whose N proteins have been sequenced (14)(15)(16)(17)(18)(19)(20). Only the N proteins from the Indiana serotype (Fig.…”

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confidence: 99%

Autoantigenicity of Ro/SSA antigen is related to a nucleocapsid protein of vesicular stomatitis virus.

Scofield

Harley

1991

Proc. Natl. Acad. Sci. U.S.A.

107

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The fine specificit of a reference human anti-Ro/SSA autoantibody-containing serum has been analyzed by using sequential overlapping octapeptides from the human 60-kDa Ro/SSA antigen. From preliminary data, the most antigenic octapeptide in the carboxyl-terminal 120 amino acid residues of Ro/SSA shares seven ofeight amino acids with the nucleocapsid (N) protein from the Indiana serotype of vesicular stomatitis virus. A sequence comparison of Ro/SSA and N has unexpectedly revealed six small peptides shared by Ro/SSA and N. Fine specificity analysis with 531 octapeptides from the Ro/SSA sequence demonstrates that five of the six shared small peptides are bound by anti-Ro/SSA (P = 0.00017). A more powerful association is not present in 12,476 protein sequences similarly evaluated. In addition, the inclusion of single-residue gaps in Ro/SSA enlarges the sequence similarity of Ro/SSA and N for three of the five small shared antigenic peptides. This additional level of sequence similarity between Ro/SSA and N is unlikely to be the result of chance (P < 0.0002). While a number of models may explain these data, including independent immune responses to N and

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“…However, the NS proteins migrate on SDSpolyacrylamide gels with apparent molecular weights of 40000 to 70000, depending on the degree of bisacrylamide cross-linking (Bell et al, 1984;Bell & Prevec, 1985;Kingsford & Emerson, 1980;Knipe et al, 1975 ;Obijeski et al, 1974;Wunner & Pringle, 1972). The aberrant mobility of NS is clearly seen when it migrates more slowly than the N protein, which has a molecular weight of 47300 (IND serotype; Gallione et al, 1981) or 47800 (NJ serotype; Banerjee et al, 1984); e.g. in the studies reported by Lesnaw et al (1979), Maack & Penhoet (1980), Patton et al (1984) and Pringle et al (1981).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Mutations Responsible for the Electrophoretic Mobility Differences in the NS Proteins of Vesicular Stomatitis Virus New Jersey Complementation Group E Mutants

Rae¹,

Elliott²

1986

Journal of General Virology

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SUMMARYTemperature-sensitive (ts) mutants of vesicular stomatitis virus, New Jersey serotype, classified in complementation group E contain lesions in the NS gene, which manifest as marked electrophoretic mobility differences of the mutant NS proteins in SDS-polyacrylamide gels. We have cloned full-length cDNA copies of the mutant NS mRNAs, and have determined their nucleotide sequences, tsE1 and tsE3 had single nucleotide changes, and tsE2 had two nucleotide changes, compared to the wild-type NS gene.

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Complete nucleotide sequence of the mRNA coding for the N protein of vesicular stomatitis virus (New Jersey serotype)

Cited by 46 publications

References 12 publications

Conservation of Potential Phosphorylation Sites in the NS Proteins of the New Jersey and Indiana Serotypes of Vesicular Stomatitis Virus

Conservation of Potential Phosphorylation Sites in the NS Proteins of the New Jersey and Indiana Serotypes of Vesicular Stomatitis Virus

Autoantigenicity of Ro/SSA antigen is related to a nucleocapsid protein of vesicular stomatitis virus.

Characterization of the Mutations Responsible for the Electrophoretic Mobility Differences in the NS Proteins of Vesicular Stomatitis Virus New Jersey Complementation Group E Mutants

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