Complete nucleotide sequence of strawberry vein banding virus Chinese isolate and infectivity of its full-length DNA clone

Abstract

BackgroundStrawberry vein banding virus (SVBV) is a double-stranded DNA plant virus, which has been found in North America, Australia, Brazil, Japan, Europe and several provinces of China. Infected strawberry plants exhibit mild vein-banding symptoms and chlorosis along the veins. It is one of the most economically important diseases in Asiatic, European and North American strawberry-growing areas.FindingsThe complete genome of an SVBV Chinese isolate (SVBV-CN) was isolated and cloned from a naturally infected strawberry (Fragaria × ananassa cv. Sachinoka) sample found in Shenyang city of Liaoning province. Sequence analysis revealed a complete genome of 7864 nucleotides (nts) that indicated SVBV-CN was most closely related to SVBV from the United States (SVBV-US) with a sequence similarity of 85.8 %. Two major clades were identified based on phylogenetic analysis of the complete genome sequences of caulimoviruses. SVBV-CN clustered together with SVBV-US, whereas other caulimoviruses formed a separate branch. Agrobacterium-mediated inoculation of Fragaria vesca with an infectious clone of SVBV-CN results in systemic infection with distinct symptoms of yellowing bands along the main leaf veins. This suggests that the SVBV-CN infectious clone can recapitulate the symptoms observed in naturally infected strawberries, and therefore is likely the causal agent of the original disease observed in strawberries. Furthermore, strawberry plants inoculated with the infectious clone using vacuum infiltration developed symptoms with a very high infection rate of 86–100 % in 4-5 weeks post-inoculation. This compares to an infection rate of 20–40 % in 8–9 weeks post-inoculation using syringe-inoculation.ConclusionsThe complete nucleotide sequence of SVBV from a naturally infected strawberry was determined. Agroinfiltration of strawberry plants using an infectious clone of SVBV-CN resulted in symptoms typically found in infected strawberries from Shenyang city of Liaoning province in China. This is the first report describing an infectious clone of SVBV-CN, and that vacuum infiltration can be potentially used as a new and highly efficient means for inoculation of strawberry plants.Electronic supplementary materialThe online version of this article (doi:10.1186/s12985-016-0624-1) contains supplementary material, which is available to authorized users.

RNA silencing machinery contributes to inability of BSBV to establish infection in Nicotiana benthamiana: evidence from characterization of agroinfectious clones of Beet soil-borne virus

“…Agroinoculation of virus is not always successful on N. benthamiana , as already described by other studies [49, 50]. Despite its susceptibility to many viruses [51], this plant species is not host for all of them thanks to, inter alia, its antiviral RNA silencing.…”

Strawberry Vein Banding Virus P6 Protein Is a Translation Trans-Activator and Its Activity Can be Suppressed by FveIF3g

“…For the investigation of the function of SVBV-encoded proteins, a series of plant expression vectors were constructed. Sources of SVBV US isolate (SVBV-US), China isolate (SVBV-SY), Cauliflower mosaic virus (CaMV), SVBV-US infectious clone (pSVBV-US), and expression vectors pCAM-P19, pCAM-2b, pCAM-P1, pCAM-P2, pCAM-P3, pCAM-P4, pCAM-P5, pCAM-P6, and pCAM-P6-GFP were reported previously [3,11,12]. To determine the interaction between SVBV P6 and FveIF3g, the full length of the eukaryotic cell translation initiation factor gene ( FveIF3g ) was PCR amplified from a cDNA that was taken from a total RNA sample isolated from strawberry ( F. Vesca ) leaf tissues.…”

“…Strawberry vein banding virus (SVBV) is an important plant virus infecting strawberries. SVBV is now widely distributed in many countries worldwide, including Australia, Japan, multiple European countries, The United States of America, and China, and causes severe loss of strawberry production [1,2,3]. SVBV is known to be transmitted by several aphid species, including Chaetosiphon sp., in a semi-persistent manner [4].…”

Functional Analysis of a Viral Promoter From the Strawberry Vein Banding Virus (SVBV) Chinese Isolate

“…The 35S promoter was originally identi ed in the CaMV genome, a virus known to infect mainly Cruciferae plants. Although both Strawberry vein banding virus (SVBV) and CaMV belong to the genus Caulimovirus, family Caulimoviridae [11][12][13], SVBV infects mainly strawberry and causes yellow vein banding and leaf twisting symptoms [11,12,14]. Genome of SVBV is a circular double stranded DNA of about 8 kilobases (kb) long and contains seven open reading frames (ORFs) [12].…”