Complete nucleotide sequence of dengue type 3 virus genome RNA

Osatomi, Kiyoshi; Sumiyoshi, Hideo

doi:10.1016/0042-6822(90)90037-r

Cited by 150 publications

(84 citation statements)

References 6 publications

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“…Alignment of the amino acid sequences of the E proteins of flaviviruses within the type-specific hypervariable domain proposed for TBE and dengue viruses. Sequences of LI (Shiu et al, 1991), TBE (Mandl et al, 1988;Ptetnev et al, 1990), LGT (Mandl et al, 1991), DEN1 (Mason et al, 1987), DEN2 (Deubel et al, 1986;Hahn et al, 1988), DEN3 (Osatomi & Sumiyoshi, 1990), DEN4 (Zhao et al, 1986), West Nile (WN) (Castle et al, 1985), Kunjin (KUN) (Coia et al, 1988), Japanese encephalitis (JE) (Sumiyoshi et al, 1987), Murray Valley encephalitis (MVE) (Dalgarno et al, 1986), St Louis encephalitis (SLE) (Trent et al, 1987) and yellow fever (Rice et al, 1985) viruses are aligned. The amino acids are numbered according to their positions in the respective E proteins.…”

Section: S S T ---A 232 Mve 228 P As T ---E 232 Sle 228 P At T ---D 2mentioning

confidence: 99%

Envelope protein sequences of dengue virus isolates TH-36 and TH-Sman, and identification of a type-specific genetic marker for dengue and tick-borne flaviviruses

Shiu

Jiang

Porterfield³

et al. 1992

Journal of General Virology

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Complementary DNAs were synthesized from the envelope protein genes of two isolates of dengue virus (TH-36 and TH-Sman, previously suggested as possible dengue virus type 5 and dengue virus type 6 respectively) and amplified by the polymerase chain reaction using sense and antisense primers designed from conserved dengue virus gene sequences. The amplified cDNA clones were sequenced in both directions by double-stranded dideoxynucleotide sequencing. Alignment with published dengue virus sequences enabled us to assign these viruses accurately to classified serotypes, confirming that TH-36 and THSman are strains of dengue virus type 2 and dengue virus type 1 respectively. Amino acid changes between the proteins encoded by these two isolates and strains of their respective serotypes may account for the significant antigenic differences observed during previous serological typing of these viruses. Moreover, sequence alignment of flavivirus envelope proteins revealed a hypervariable region, within which members of the dengue and tick-borne virus antigenic complexes show unique peptide sequences. This type-specific hypervariable domain may be useful as a genetic marker for typing dengue and tick-borne flaviviruses.Dengue viruses, mosquito-borne members of the family Flaviviridae (Westaway et al., 1985), are responsible for classic dengue fever and for the associated conditions dengue haemorrhagic fever (DHF) and dengue shock syndrome, each of which has a high level of morbidity and mortality in the tropics. There is evidence suggesting that the more severe forms of dengue fever occur when individuals (usually children) with cross-reactive but non-protective antibodies, acquired maternally or induced by prior infection with a different serotype, are naturally exposed to dengue virus infection (Halstead, 1981(Halstead, , 1988. Despite much research, effective dengue virus vaccines are not yet available, so the antigenic properties of dengue viruses have received much attention.Currently, four distinct dengue virus serotypes (types 1 to 4; DEN1 to DEN4) can be distinguished by complement fixation (CF), haemagglutination-inhibition and neutralization tests (Porterfield, 1980). Two Thai isolates are of particular interest. Strain TH-36 wasThe sequence data reported in this article have been deposited with the DDBJ, EMBL and GenBank databases under the accession numbers D01073 (TH-Sman) and D01074 (TH-36). isolated in 1958 by Hammon and co-workers from a patient with DHF in Bangkok, and TH-Sman was isolated from a similar patient by Dr Sman Vardhanabhuti. Strain TH-36 was initially identified as DEN2 and TH-Sman as DEN 1, but both were shown to differ to a significant degree from the respective prototype strains by CF, plaque neutralization, immunodiffusion and immunoelectrophoresis tests (Hammon et al., 1961; Hammon & Sather, 1964a, b; Ibrahim & Hammon, 1968a, b;Ibrahim et al., 1968). Tests using the soluble complement-fixing antigen also showed that strain THSman differed from the prototype DEN1 virus, but TH-36 was ind...

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Section: S S T ---A 232 Mve 228 P As T ---E 232 Sle 228 P At T ---D 2mentioning

confidence: 99%

Envelope protein sequences of dengue virus isolates TH-36 and TH-Sman, and identification of a type-specific genetic marker for dengue and tick-borne flaviviruses

Shiu

Jiang

Porterfield³

et al. 1992

Journal of General Virology

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“…For each isolate, we sequenced a 1,050-nucleotide genomic region (positions 95-1,144) that encompasses the complete C, CЈ, preM, and M protein genes and part of the E protein gene. The 1,050-nucleotide sequences of the nine Malaysian isolates and that of a Philippine DEN-3 isolate, H-87, for which the entire nucleic acid sequence was determined, 17 are shown in Figure FIGURE 2. Comparison of the deduced amino acid sequences of C, CЈ, preM, and M proteins and partial E protein from 10 dengue type 3 virus strains.…”

Section: Resultsmentioning

confidence: 99%

“…The nucleic acid sequences of the C and CЈ protein genes have not been reported for most DEN-3 isolates, as they have for H-87. 17 However, the sequences of the preM, M, and E genes from 23 distinct DEN-3 isolates that have been obtained from various geographic regions and at various times were available. 4 Thus, we aligned the 720-nucleotide sequences encompassing the preM and M protein genes and part of the E protein gene from the nine Malaysian isolates and 23 previously established isolates (Table 3).…”

Section: Resultsmentioning

confidence: 99%

Type-3 dengue viruses responsible for the dengue epidemic in Malaysia during 1993-1994.

Kobayashi¹,

Thayan²,

Sugimoto³

et al. 1999

The American Journal of Tropical Medicine and Hygiene

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Abstract. To characterize the dengue epidemic that recently occurred in Malaysia, we sequenced cDNAs from nine 1993-1994 dengue virus type-3 (DEN-3) isolates in Malaysia (DEN-3 was the most common type in Malaysia during this period). Nucleic acid sequences (720 nucleotides in length) from the nine isolates, encompassing the precursor of membrane protein (preM) and membrane (M) protein genes and part of the envelope (E) protein gene were aligned with various reference DEN-3 sequences to generate a neighbor-joining phylogenetic tree. According to the constructed tree, the nine Malaysian isolates were grouped into subtype II, which comprises Thai isolates from 1962 to 1987. Five earlier DEN-3

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“…The gene order is 5 '-C-PrM(M)-E-NS1-NS2A-NS2B-N53-NS4A-NS4B-N55-3', similar to all sequenced members of the genus flavivirus (35). Representative strains of all 4 serotypes of dengue virus have been sequenced (4,7,11,18,33,44).…”

mentioning

confidence: 99%

Severity‐Related Molecular Differences among Nineteen Strains of Dengue Type 2 Viruses

Pandey

Igarashi

2000

Microbiology and Immunology

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Comparative nucleotide sequencing was carried out on dengue type 2 virus (DEN-2) strains isolated from patients in Northeast Thailand during the epidemic season in 1993. The patients exhibited different clinical manifestations ranging from dengue fever (DF) to dengue haemorrhagic fever (DHF)/dengue shock syndrome (DSS). The results classified 19 DEN-2 strains into 3 subtypes according to nonsynonymous amino acid replacements. The strain isolated from a DSS patient eliciting secondary serological response belonged to subtype I, whereas 13 strains isolated from DHF patients with secondary response and 2 strains from DF patients with primary response belonged to subtype II. On the other hand, 3 strains isolated from DF cases evoking either primary or secondary response belonged to subtype III. These results suggest that subtype III virus infection could result in clinically milder manifestation irrespective of the serological response compared with subtype I or II viruses. The RNA secondary structure predicted for the 3' noncoding region showed 4 different structures (A, B, C, and D). The result also indicates that different subtypes of DEN-2 serotypes are circulating in a single epidemic in Thailand.

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Complete nucleotide sequence of dengue type 3 virus genome RNA

Cited by 150 publications

References 6 publications

Envelope protein sequences of dengue virus isolates TH-36 and TH-Sman, and identification of a type-specific genetic marker for dengue and tick-borne flaviviruses

Envelope protein sequences of dengue virus isolates TH-36 and TH-Sman, and identification of a type-specific genetic marker for dengue and tick-borne flaviviruses

Type-3 dengue viruses responsible for the dengue epidemic in Malaysia during 1993-1994.

Severity‐Related Molecular Differences among Nineteen Strains of Dengue Type 2 Viruses

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