Type-3 dengue viruses responsible for the dengue epidemic in Malaysia during 1993-1994.

Kobayashi, Nozomu; Thayan, Ravindran; Sugimoto, Chihiro; Oda, Kazumasa; Saat, Zainah; Balasubramaniam, Vijayamalar; Sinniah, M; Igarashi, Akira

doi:10.4269/ajtmh.1999.60.904

Cited by 19 publications

(11 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, earlier DENV-3 Malaysian isolates belonged to subtype I (Kobayashi et al, 1999). Similar studies in India suggest that genotype V of DENV-2 was replaced by genotype IV between 1967 and 1996 (Singh et al, 1999).…”

Section: Contributions Of Phylogenetics To the Understanding Of Dementioning

confidence: 74%

Molecular evolution of dengue viruses: Contributions of phylogenetics to understanding the history and epidemiology of the preeminent arboviral disease

Weaver

Vasilakis

2009

Infection, Genetics and Evolution

394

380

View full text Add to dashboard Cite

Dengue viruses (DENV) are the most important arboviral pathogens in tropical and subtropical regions throughout the world, putting at risk of infection nearly a third of the global human population. Evidence from the historical record suggests a long association between these viruses and humans. The transmission of DENV includes a sylvatic, enzootic cycle between nonhuman primates and arboreal mosquitoes of the genus Aedes, and an urban, endemic/epidemic cycle between Aedes aegypti, a mosquito with larval development in peridomestic water containers, and human reservoir hosts. DENV are members of the genus Flavivirus in the Family Flaviviridae and comprise of 4 antigenically distinct serotypes (DENV-1-4). Although they are nearly identical epidemiologically, the 4 DENV serotypes are genetically quite distinct. Utilization of phylogenetic analyses based on partial and/or complete genomic sequences has elucidated the origins, epidemiology (genetic diversity, transmission dynamics and epidemic potential), and the forces that shape DENV molecular evolution (rates of evolution, selection pressures, population sizes, putative recombination and evolutionary constraints) in nature. In this review, we examine how phylogenetics have improved understanding of DENV population dynamics and sizes at various stages of infection and transmission, and how this information may influence pathogenesis and improve our ability to understand and predict DENV emergence.

show abstract

Section: Contributions Of Phylogenetics To the Understanding Of Dementioning

confidence: 74%

Molecular evolution of dengue viruses: Contributions of phylogenetics to understanding the history and epidemiology of the preeminent arboviral disease

Weaver

Vasilakis

2009

Infection, Genetics and Evolution

394

380

View full text Add to dashboard Cite

show abstract

“…It is fairly well established that such acquired changes affect the dengue disease severity [24,27]. Coupled with this, introduction of newer viral strains by cross-border transmission causes unprecedented changes in dengue disease spectrum in a region [13,28]. A keen monitoring of these events would be a major requirement for successful dengue surveillance and control programs.…”

Section: Discussionmentioning

confidence: 99%

Lineage shift in Indian strains of Dengue virus serotype-3 (Genotype III), evidenced by detection of lineage IV strains in clinical cases from Kerala

Manakkadan

Joseph

Prasanna

et al. 2013

Virol J

View full text Add to dashboard Cite

BackgroundLocal epidemiology of Dengue is defined by the genetic diversity of the circulating Dengue virus (DENV) strains. This important information is not available for the virus strains from most parts of the Indian subcontinent. The present study focused on the genetic diversity of the serotype 3 DENV strains (DENV-3) from India.ResultsA total of 22 DENV-3 strains identified by reverse-transcription PCR analysis of serum samples from 709 patients were studied. These samples were collected over a period of 4 years (2008–2011) from dengue fever suspected patients from Kerala, a dengue endemic state in South India. Comparison of a 1740bp nucleotide sequence of the viral Capsid-Pre-membrane-Envelope coding region of our strains and previously reported DENV-3 strains from India, South Asia and South America revealed non-synonymous substitutions that were genotype III-specific as well as sporadic. Evidence of positive selection was detected in the I81 amino acid residue of the envelope protein. Out of the 22 samples, three had I81A and 18 had I81V substitutions. In the phylogenetic analysis by maximum likelihood method the strains from Kerala clustered in two different lineages (lineage III and IV) within genotype III clade of DENV-3 strains. The ten strains that belonged to lineage IV had a signature amino acid substitution T219A in the envelope protein. Interestingly, all these strains were found to be closely related to a Singapore strain GU370053 isolated in 2007.ConclusionsOur study identifies for the first time the presence of lineage IV strains in the Indian subcontinent. Results indicate the possibility of a recent exotic introduction and also a shift from the existing lineage III strains to lineage IV. Lineage shifts in DENV-3 strains have been attributed to dramatic increase in disease severity in many parts of the world. Hence the present observation could be significant in terms of the clinical severity of future dengue cases in the region.

show abstract

“…Several subsequent DENV-3 phylogenies based on the E gene [69,202,223–232], partial gene(s) or gene junctions (e.g., C, prM/E, NS3) [83,233–236] or complete genome [237–239] sequences have confirmed these genotypes.…”

Section: Phylogeny As a Tool To Understand Denv Epidemiology And Evolmentioning

confidence: 99%

Dengue — Quo tu et quo vadis?

Chen

Vasilakis

2011

Viruses

230

246

View full text Add to dashboard Cite

Dengue viruses (DENV) are by far the most important arboviral pathogens in the tropics around the world, putting at risk of infection nearly a third of the global human population. DENV are members of the genus Flavivirus in the Family Flaviviridae and comprise four antigenically distinct serotypes (DENV-1-4). Although they share almost identical epidemiological features, they are genetically distinct. Phylogenetic analyses have revealed valuable insights into the origins, epidemiology and the forces that shape DENV evolution in nature. In this review, we examine the current status of DENV evolution, including but not limited to rates of evolution, selection pressures, population sizes and evolutionary constraints, and we discuss how these factors influence transmission, pathogenesis and emergence.

show abstract

Type-3 dengue viruses responsible for the dengue epidemic in Malaysia during 1993-1994.

Cited by 19 publications

References 17 publications

Molecular evolution of dengue viruses: Contributions of phylogenetics to understanding the history and epidemiology of the preeminent arboviral disease

Molecular evolution of dengue viruses: Contributions of phylogenetics to understanding the history and epidemiology of the preeminent arboviral disease

Lineage shift in Indian strains of Dengue virus serotype-3 (Genotype III), evidenced by detection of lineage IV strains in clinical cases from Kerala

Dengue — Quo tu et quo vadis?

Contact Info

Product

Resources

About