Complete mitochondrial genome sequencing of Oxycarenus laetus (Hemiptera: Lygaeidae) from two geographically distinct regions of India

Abstract

Oxycarenus laetus is a seed-sap sucking pest affecting a variety of crops, including cotton plants. Rising incidence and pesticide resistance by O. laetus have been reported from India and neighbouring countries. In this study, O. laetus samples were collected from Bhatinda and Coimbatore (India). Pure mtDNA was isolated and sequenced using Illumina MiSeq. Both the samples were found to be identical species (99.9%), and the complete genome was circular (15,672 bp), consisting of 13 PCGs, 2 rRNA, 23 tRNA genes, and a 962 bp control region. The mitogenome is 74.1% AT-rich, 0.11 AT, and − 0.19 GC skewed. All the genes had ATN as the start codon except cox1 (TTG), and an additional trnT was predicted. Nearly all tRNAs folded into the clover-leaf structure, except trnS1 and trnV. The intergenic space between trnH and nad4, considered as a synapomorphy of Lygaeoidea, was displaced. Two 5 bp motifs AATGA and ACCTA, two tandem repeats, and a few microsatellite sequences, were also found. The phylogenetic tree was constructed using 36 mitogenomes from 7 super-families of Hemiptera by employing rigorous bootstrapping and ML. Ours is the first study to sequence the complete mitogenome of O. laetus or any Oxycarenus species. The findings from this study would further help in the evolutionary studies of Lygaeidae.

Strong genetic differentiation between fragmented alpine bush‐cricket populations demands preservation of evolutionary significant units

“…Since the control region is known to be highly variable in insects and sometimes even contains microsatellites (Sureshan et al, 2021; Taylor et al, 1993), a haplotype network was constructed by removing CR sequences to avoid any possible bias. Even if the number of haplotypes decreased, the topology of the network was maintained, as well as the presence of haplotypes exclusive to a single population.…”

Characterization and phylogenetic analysis of the complete mitochondrial genome sequence of Photinia serratifolia

“…To date, more than 5000 plant chloroplast genomes have been sequenced, but only about 424 plant mitogenome sequences are available ( https://www.ncbi.nlm.nih.gov/genome/organelle/ , 2/12/2022). Mitochondria are the main organelles involved in energy metabolism in eukaryotic cells 10 , 11 . In plant, mitochondria play an important role in plant productivity, development, and various biochemical processes 12 – 14 .…”

“…gov/ genome/ organ elle/, 2/12/2022). Mitochondria are the main organelles involved in energy metabolism in eukaryotic cells 10,11 . In plant, mitochondria play an important role in plant productivity, development, and various biochemical processes [12][13][14] .…”

Complete mitogenome structure and phylogenetic implications of the first Indian yak breed- Arunachali (Poephagus grunniens L.)

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