Complete mitochondrial genome of the Liuyang black goat and its phylogenetic relationship with other Caprinae

Abstract

ABSTRACT. In this study, the complete mitochondrial genome sequence of the Liuyang black goat was investigated, and phylogenetic relationships between the Liuyang black goat and other species of Caprinae were analyzed. The total length of the mitochondrial genome was 16,715 bp, which consisted of 33.50% A, 27.27% T, 25.98% C, and 13.25% G. The mitochondrial genome contained a major noncoding control region (D-loop region), two ribosomal RNA genes, 13 protein-coding genes, and 22 transfer RNA genes. Neighbor-joining and maximum-parsimony trees of Caprinae constructed using 13 mitochondrial protein-coding genes showed that the Liuyang black goat is phylogenetically closest to Hemitragus jemlahicus (the Himalayan tahr) and Blue sheep to form clade A. Tibetan antelopes clustered separately in clade B and so did sheep in clade C.

The complete mitochondrial DNA sequence of Yimeng black goat (Capra hircus) and its potential application in mutton discrimination

“…The complete mitochondrial genome consists of 37 genes and a control region (D-loop). The 37 genes include 22 tRNA genes, 13 protein-coding genes, and two rRNA genes, which is consistent with the distribution and composition of the mitochondrial genome of other vertebrates (Parma et al 2003;Sun et al 2015;Chen et al 2016;E et al 2016;Jia and Wei 2016;Liu et al 2016;Li, Liu, Sui, et al 2019;Li, Liu, Zhang, et al 2019). A neighbor-joining phylogenetic tree constructed from the complete mitochondrial genomes of 18 different species and breeds of Caprinae and one species of Antilopinae is shown in Figure 1.…”

Amino acid sequence based on Cytochrome b gene in Kejobong goat and its genetic relationships among several local goats in Asia

“…The latest study using Cyt b gene for genetic information analysis on goat was done in Iran goat [ 19 ], Chinese goat [ 20 , 21 ], Vietnamese goat [ 22 ], Turkish goat [ 23 ], Indian goat [ 24 ], and Indonesian goat [ 9 , 10 , 25 - 27 ]. The length of Cyt b gene in goat was 1140 bp forming 377 amino acids, preceded with start codon encoded by ATG, end with stop codon encoded by AGA, and located between tRNA Glu and tRNA Thr in mtDNA [ 28 - 30 ]. Cyt b is a conserved area or do not change much or do not experience base mutation, so it is more sensitive to use as a genetic marker or barcode to identify purity of species [ 31 - 33 ].…”