Complete mitochondrial genome of melon fly, Zeugodacus cucurbitae (Diptera:Tephritidae) from Kunming, Southwest China and the phylogeny within subfamily Dacinae

Abstract

The melon fly, Zeugodacus cucurbitae (Diptera:Tephritidae) is an important invasive pest and distributed throughout tropical, subtropical countries and areas. In this study, we report the complete mitochondrial genome of the fly with 15, 685 base pair in length, which includes 37 genes (the large and small ribosomal RNA subunits, 22 transfer RNA genes, 13 genes encoding mitochondrial proteins) and a noncoding A þ T-rich control region. Molecular phylogeny indicated that there was a high bootstrap value supported among Z. cucurbitae and Z. tau belonging to the Z. tau complex.

The first complete mitochondrial genome ofSphaeniscus atilius(Walker, 1849) (Diptera: Tephritidae) and implications for the phylogenetic relationships of Tephritidae

“…In the present study, the phylogenetic relationships within Tephritidae can be presented as follows: ((Bactrocera, Dacus, Zeugodacus), Felderimyia, Anastrepha), (Acrotaeniostola, (Neoceratitis, Ceratitis), Euleia, Rivellia), (Procecidochares, (Tephritis, Sphaenisscus))))), in concordance with the findings of previous phylogenetic studies [23,24,63,64]. The results showed that four genera (Felderimyia, Zeugodacus, Dacus and Bactrocera) are closely related and they gathered to form a sister group.…”

“…The complete mitogenome of S. atilius was a circular, double-stranded DNA molecule with a total length of 16, 854 bp, which was similar to that of other Tephritoidea insects analyzed, ranging from 15,117 bp (Tephritis femoralis) to 16,739 bp (Anastrepha fraterculus) (Table 1). It has the typical organization and composition of an insect mitochondrion, containing 37 genes, including 13 protein-coding genes (PCGs), 22 tRNA genes, and 2 rRNA genes, which was consistent with those described in other subfamily Tephritoidea insects, such as Bactrocera carambolae [22], Zeugodacus cucurbitae [24], Dacus haikouensis [40]. The nucleotide composition of all regions showed a strong AT bias [41], as seen in other insects [18,19,42].…”

“…The cox1 gene had the lowest Ka/Ks ratio (Ka/Ks=0.06), indicating that this gene had a relatively slow evolutionary rate [51]. This phenomenon occurred in almost all animals [52], and has been subjected to species identification and evolutionary analysis in various arthropod species in Tephritoidea, as well as like in other insects [24,53].…”

“…CC-BY 4.0 International license made available under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is Bactrocera biguttula [23], Zeugodacus cucurbitae [24], and Lepidotrigona flavibasis [25]. The aforementioned studies of mitochondrial gene sequences explored the origin and evolution of insects, explained the species and evolution of the system, and revealed the geographical distribution of intraspecific polymorphism.…”

Comparative multi‐tissue analyses identify testis‐specific serine/threonine protein kinase (TSSK) genes involved in male fertility in the melon fly Zeugodacus cucurbitae

“…Zeugodacus cucurbitae Coquillett, also known as the melon fly, is an economically important pest species that is found throughout subtropical and tropical regions. 1 While Z. cucurbitae can attack roughly 130 host plants, snap melons, musk melons, and bitter gourds are their preferred hosts. 2 Owing to their highly destructive, invasive, polyphagous characteristics, Z. cucurbitae are considered to be a critical quarantine pest.…”