Complete glutathione system in probiotic Lactobacillus fermentum ME-3

Kullisaar, Tiiu; Songisepp, Epp; Aunapuu, Marina; Kilk, Kalle; Arend, Andres; Mikelsaar, Marika; Rehema, Aune; Zilmer, Mihkel

doi:10.1134/s0003683810050030

Cited by 67 publications

(60 citation statements)

References 27 publications

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“…Some lipid hydroperoxides in food may escape from reduction by glutathione peroxidase in the GI tract and enter the circulation [31]. Consumption of antioxidant probiotic LfME-3, which produces glutathione and has complete glutathione redox cycle enzymes (Gp x and GRed), may contribute to the reduction of lipid hydroperoxides in the GI tract and in hepatocytes and prevent them from entering the circulation [32].…”

Section: Discussionmentioning

confidence: 99%

An antioxidant probiotic reduces postprandial lipemia and oxidative stress

Kullisaar¹,

Shepetova

Zilmer³

et al. 2010

Open Life Sciences

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Reducing postprandial oxidative stress (OxS), decreasing postprandial blood triglyceride level (TG) and improving lipoprotein status is likely to have a preventive impact on the development of cardiovascular disease (CVD). Previously we have shown that the antioxidant probiotic Lactobacillus fermentum ME-3 (DSM14241) is characterized by antiatherogenic effects. This randomized double-blind placebo-controlled study evaluated the influence of kefir enriched with an antioxidative probiotic L. fermentum ME-3 (LfKef) on postprandial OxS, blood TG response and lipoprotein status. 100 clinically healthy subjects were recruited into the study. Blood parameters of postprandial OxS, TG and lipoprotein status were determined by oxidized LDL, baseline diene conjugation in LDL (BDC-LDL), oxidized LDL complex with beta-2 glycoprotein (Beta2-GPI-oxLDL), paraoxonase (PON) activity, LDL-Chol, HDL-Chol and TG. To evaluate general body postprandial OxS-load we measured 8-isoprostanes (8-EPI) in the urine. Consumption of LfKef significantly reduced the postprandial level of oxidized LDL, BDC-LDL, Beta2-GPI-oxLDL, urinary 8-isoprostanes and postprandial TG and caused a significant increase in HDL-Chol and PON activity. This is the first evidence that kefir enriched with an antioxidant probiotic may have a positive effect on both postprandial OxS and TG response as well as on lipoprotein status.

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Section: Discussionmentioning

confidence: 99%

An antioxidant probiotic reduces postprandial lipemia and oxidative stress

Kullisaar¹,

Shepetova

Zilmer³

et al. 2010

Open Life Sciences

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“…[23]) and GSH protected the 323 cells against acid stress [24] or heat stress and prevented peroxida-324 tion of membrane fatty acids [25]. Similarly, a complete glutathione 325 system against oxidative stress was demonstrated in Lactobacillus 326 fermentum [26]. Selenite resistance induced stress response sys-327 tems that lead up-regulation of GR production In the Gram-positive 328 bacterium E. faecalis, GR has been purified to homogeneity [27]; 329 meanwhile, no GR activity has been described in E. faecium until 330 now.…”

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confidence: 99%

“…25 Selenium status of people varies by country: intakes are usually 26 high in North America and Japan, varies in China, while lower in 27 Europe (especially in Eastern Europe) and Australia. Low selenium Abbreviations: DTNB, 5,5 -dithobis-(2-nitrobenzoic acid); GR, glutathione reductase; GSH, glutathione; TEA, triethanolamine.…”

mentioning

confidence: 99%

Selenite-stress selected mutant strains of probiotic bacteria for Se source production

Pusztahelyi

Kovács

Pócsi

et al. 2015

Journal of Trace Elements in Medicine and Biology

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“…A food containing an antioxidant LAB strain that has been proven to contain glutathione and has the ability to synthesize and handle glutathione [10] has shown clinically relevant positive effects in humans [4,18]. Now we are able to prove with MALDI-TOF mass spectrometry analysis that the concentration of glutathione in the Figure 3.…”

Section: Discussionmentioning

confidence: 73%

“…manganese superoxide dismutase, eliminates hydroxyl radicals and contains a whole glutathione (GSH) system: synthesis, transport and redox cycling [4,[8][9][10]. The latter is important as without GSH and the glutathione redox cycle, LAB cannot effectively protect themselves against reactive oxygen species (oxidative stress) as the antioxidative potency in lactobacilli has a significant positive correlation with cellular GSH levels [11,12].…”

mentioning

confidence: 99%

Proteomic proof that a probiotic elevates glutathione level in human serum

et al. 2015

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Lactobacillus fermentum ME-3 (LfME-3) has been proven to synthesize and secrete glutathione. A regular use of the foods fermented by it has shown a favourable influence on human lipid profiles and several antioxidant parameters. We administered the LfME-3-fermented kefir for 14 days to 43 human subjects and evaluated their serum with MALDI-TOF mass spectrometer at the beginning and end of the test period. We found an increase of the peak at m/z 308 (corresponding to glutathione) and a new peak at m/z 1467.

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Complete glutathione system in probiotic Lactobacillus fermentum ME-3

Cited by 67 publications

References 27 publications

An antioxidant probiotic reduces postprandial lipemia and oxidative stress

An antioxidant probiotic reduces postprandial lipemia and oxidative stress

Selenite-stress selected mutant strains of probiotic bacteria for Se source production

Proteomic proof that a probiotic elevates glutathione level in human serum

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