Complete genome sequences of two new variants of Grapevine rupestris stem pitting-associated virus and comparative analyses

Abstract

Grapevine rupestris stem pitting-associated virus (GRSPaV), a member of the genus Foveavirus within the family Flexiviridae, is the putative causal agent of the disease Rupestris stem pitting (RSP) of grapevines. GRSPaV comprises a family of variants whose pathological characteristics have not been determined. Recently, many of the indicator 'St George' plants (Vitis rupestris) used throughout the world to index RSP tested positive for GRSPaV. This finding questions the validity of past biological indexing results. In this work, a representative genomic region of GRSPaV was first sequenced from ten 'St George' plants from two sources and it was demonstrated that nine of them carried a new variant, GRSPaV-SG1. The genomes of GRSPaV-SG1 and GRSPaV-BS from 'Bertille Seyve 5563' plants were sequenced, revealing a genome structure identical to that of GRSPaV-1. It was demonstrated experimentally that infection of 'St George' plants with GRSPaV-SG1 is asymptomatic and thus it is proposed that GRSPaV-SG1 infection should not have interfered with the outcome of past indicator indexing. This represents the first attempt to link a GRSPaV variant with pathological properties. Vitis rupestris 'St George' is the standard biological indicator used worldwide to diagnose RSP. In 1995, when we started to develop RT-PCR assays, 'St George' plants were used as negative controls. Unexpectedly, these 'St George' plants tested positive for GRSPaV. This finding was confirmed by consistent detection of GRSPaV by using RT-PCR and Western blotting in 'St George' plants obtained from two sources; among the 29 'St George' plants tested, 23 were positive for GRSPaV (Meng et al., 2000, 2003). Likewise, Minafra et al. (2000) detected GRSPaV in the 'St George' selection maintained at the University of Bari, Italy. This finding triggered several questions. Were these 'St

Detecção e identificação molecular de vírus associados a videiras sintomáticas e assintomáticas

“…ALABI et al (2010) analisaram, com base na análise filogenética do gene da proteína capsidial, 84 isolados de RSPaV, incluindo nove isolados brasileiros, caracterizados por RADAELLI et al (2009), que se agruparam com a estirpe típica "GRSPaV-1" (isolados 420A, MG, CF210, CF208, MH, CF195-2, PN e CF207) e com a estirpe "GRSPaV-BS" (isolado CF195). MENG et al (2005b) demonstraram experimentalmente que a infecção da videira cv. 'St.…”

Viruses and Viral Diseases of Grapevine (Vitis Sp.)

“…Rugose wood complex (RWC) скла-дається з чотирьох окремих захворювань, котрі дуже поширені у всьому світі, для них є характерним утворення ямок та борозен на здерев'янілому штамбі (рис. 3) [18,34,42].…”

“…До складу RWC входять представники двох самостійних родів Foveavirus (RSPaV) та Vitivirus (GVA, GVB) родини Betaflexiviridae порядка Tymovirales [25,31,34]: Rupestris stem pitting associated virus (RSPaV) -вірус борознистості деревини Рупестріс (індикатор V. rupestris дю Ло); Grapevine virus B (GVB) -Corky bark -вірус В винограду -опробковіння кори (індикатор LN 33) [19]; Grapevine virus A (GVA) -Kober stem grooving -вірус A винограду -наявність виямок у деревині Кобера (індикатор Кобер 5ББ). LN 33 stem grooving -на-явність ямок у деревині ЛН (індикатор LN 33) [8].…”

Variabilidade do gene da proteína capsidial de três espécies virais que infectam videiras no Brasil

“…Infecções mistas com outros vírus são comumente detectadas em videira, o que dificulta a definição das propriedades biológicas das diferentes variantes deste vírus (Meng et al 1999, Meng et al, 2005b. Recentemente, Nakaune et al (2008) enfatizaram a diversidade viral associada ao lenho rugoso, destacando a importância de estudos de variabilidade genética por meio de análises de sequência de isolados de RSPaV.…”