Complete Genome Sequences of Two Vibrio natriegens Bacteriophages

Harris, Meghan T; Ho, Tereasa Ching; Fruchtman, Harry; Garin, Mira E.; Kubatin, Victor; Lu, T.; Xue, Lingyan; Marr, Michael T.

doi:10.1128/mra.01133-20

Cited by 3 publications

(2 citation statements)

References 13 publications

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“…Interestingly, these bacteriophages give efficacious results in controlling the infections caused by Vibrio , suggesting that phagevB_VpS_PG28 may prove a biological control agent. Complex evolutionary relationships can be predicted between these two phages as both phages were isolated from different territories of the world [ 40 ].…”

Section: Resultsmentioning

confidence: 99%

Characterization and complete genome sequence analysis of a newly isolatedphage against Vibrio parahaemolyticus from sick shrimp in Qingdao, China

Tian

et al. 2022

PLoS ONE

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Foodborne diseases have become a serious havoc, where antimicrobial resistance is throwing significant challenges on daily basis. With the increase of drug-resistant bacteria and food-borne infection associated with Vibrio parahaemolyticus, new and effective strategies were needed to control the emergence of vibriosis. Lytic bacteriophages come up as a promising way to resist the pathogenic population in various applications. In this study, a V. parahaemolyticus specific phage vB_VpS_PG28 was isolated from sewage in the seafood market. Results showed vB_VpS_PG28, is strictly a lytic bacteriophage and has a relatively large burst size of 103 plaque-forming units per infected cell. Comparative genomic and bioinformatic analyses proved that vB_VpS_PG28 is a new bacteriophage that had a homologous relation with Vibrio phages of family Siphoviridae, especially with phage VH2_2019, but transmission electron microscopy of vB_VpS_PG28 morphology characterized its morphology is similar to that of Myoviridae family. In silico analysis indicated that the vB_VpS_PG28 genome consists of 82712 bp (48.08% GC content) encoding 114 putative ORFs without tRNA,and any gene associated with resistance or virulence factors has not been found. The bacteriophage in the present study has shown significant outcomes in order to control bacterial growth under in vitro conditions. Thus, we are suggesting a beneficiary agent against foodborne pathogens. Further, to ensure the safe usage of phage oral toxicity testing is recommended.

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Section: Resultsmentioning

confidence: 99%

Characterization and complete genome sequence analysis of a newly isolatedphage against Vibrio parahaemolyticus from sick shrimp in Qingdao, China

Tian

et al. 2022

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…The functions of most open reading frames (ORFs) in phage genome sequences in the GenBank database are currently unknown ( 7 ). Limited by gene editing technology and other factors, the genomes of approximately 66 Vibrio phages have been sequenced, but most research on functional genomics has stagnated at homology comparisons, causing studies on the functional genomics of Vibrio phages to lag behind the discovery of new phages and the sequencing of their genomes ( 8 – 10 ). At present, phage genome editing strategies can be classified into four categories: in vitro oligonucleotide (oligo) splicing, homologous recombination-mediated in vivo PCR DNA fragment splicing, endogenous and exogenous homologous recombination system-mediated editing, and jointly mediated editing by clustered regularly interspaced short palindromic repeat/CRISPR-associated gene (CRISPR–Cas) systems and homologous recombination.…”

Section: Introductionmentioning

confidence: 99%