Complete genome sequences of two biologically distinct isolates of Asparagus virus 1

Abstract

The complete genome sequences of two asparagus virus 1 (AV-1) isolates differing in their ability to cause systemic infection in Nicotiana benthamiana were determined. Their genomes had 9,741 nucleotides excluding the 3'-terminal poly(A) tail, encoded a polyprotein of 3,112 amino acids, and shared 99.6 % nucleotide sequence identity. They differed at 37 nucleotide and 15 amino acid sequence positions (99.5 % identity) scattered over the polyprotein. The closest relatives of AV-1 in amino acid sequence identity were plum pox virus (54 %) and turnip mosaic virus (53 %), corroborating the classification of AV-1 as a member of a distinct species in the genus Potyvirus.

Host range and molecular and ultrastructural analyses of Asparagus virus 1 pathotypes isolated from garden asparagus Asparagus officinalis L.

“…Among the German AV1 isolates, two pathotypes have been identified that differed in their ability to infect Nicotiana benthamiana (Rabenstein et al, 2007). On the molecular level, 37 non-synonymous nucleotide substitutions, resulting in 15 amino acid sequence differences, spanning the whole coding region were identified between them (Blockus et al, 2015).…”