Molecular identification and biological characterization of a new potyvirus in lettuce

Ciuffo, M.; Mammella, Marco; Vallino, Marta; Caciagli, P.; Turina, Massimo

doi:10.1007/s00705-016-2920-x

Cited by 8 publications

(5 citation statements)

References 20 publications

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“…Isolate 7091 shared 98% nt identity (99% aa identity) with a potyvirus recently described as lettuce Italian necrotic virus (LINV; accession ; Ciuffo et al ., ). For isolate 7098, the closest virus identified from whole genome sequence analysis was vanilla distortion mosaic virus (VDMV; 55% nt identity, 62% aa identity).…”

Section: Resultsmentioning

confidence: 97%

“…The molecular studies showed that isolates 7091 and 7098, collected on lettuce plants with symptoms in southeastern France in 2012, belonged to two different potyvirus species. Isolate 7091 shared 98% nt sequence identity over the whole genome with a potyvirus isolate collected in Italy in July 2014 in L. sativa ‘Romana’ (Ciuffo et al ., ) and described as a member of the tentative new species LINV. The Italian isolate induces necrosis and leaf distortion on lettuce, similar to the symptoms observed for isolate 7091.…”

Section: Discussionmentioning

confidence: 99%

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Molecular and biological characterization of two potyviruses infecting lettuce in southeastern France

et al. 2017

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Several potyviruses affect lettuce (Lactuca sativa) and chicory (Cichorium spp.) crops worldwide and are important constraints for production because of the direct losses that they induce and/or because of their seed transmission. Here, the molecular and biological properties are described of two potyviruses that were recently isolated from lettuce plants showing mosaic or strong necrotic symptoms in an experimental field in southeastern France. The first potyvirus belongs to the species Endive necrotic mosaic virus and is present in a large number of wild plant species, especially Tragopogon pratensis. It is unable to infect lettuce cultivars with a resistance to Turnip mosaic virus that is present in many European cultivars and probably conferred by the Tu gene. The second potyvirus belongs to the tentative species lettuce Italian necrotic virus and was not observed in wild plants. It infected all tested lettuce cultivars. Wild accessions of Lactuca serriola, Lactuca saligna, Lactuca virosa and Lactuca perennis were identified as resistant to one or the other potyvirus and could be used for resistance breeding in lettuce. No resistance against these two potyviruses was observed in the tested Cichorium endivia cultivars. In contrast, all tested Cichorium intybus cultivars or accessions were resistant.

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Section: Resultsmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Molecular and biological characterization of two potyviruses infecting lettuce in southeastern France

et al. 2017

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“…Lettuce ( Lactuca sativa , family Asteraceae) crops are affected by several potyviruses (genus Potyvirus ; family Potyviridae ), including Lettuce mosaic virus (LMV), Turnip mosaic virus (TuMV), Bidens mottle virus (BiMoV), Endive necrotic mosaic virus (ENMV) and lettuce Italian necrotic virus (LINV) (Christie et al ., ; Zink et al ., ; Zitter & Guzman, ; Provvidenti & Hampton, ; Blancard et al ., ; Ciuffo et al ., ; Desbiez et al ., ). LINV was recently described in lettuce crops in Italy and southeast France and induces severe necrosis and plant wilting, leaf distortion and/or vein clearing.…”

Section: Introductionmentioning

confidence: 98%

“…However, experimental inoculations showed that it can infect plants of other species in the family Asteraceae ( Calendula spp., Cichorium spp., Helianthus annuus , Tragopogon pratensis and Zinnia elegans ) and Nicotiana benthamiana (Desbiez et al ., ). Transmission by Myzus persicae (Homoptera: Aphididae) was demonstrated experimentally (Ciuffo et al ., ; Desbiez et al ., ). LINV is phylogenetically related to LMV in the genus Potyvirus .…”

Section: Introductionmentioning

confidence: 98%

Growth stage‐dependent resistance to the potyviruses lettuce Italian necrotic virus and Lettuce mosaic virus displayed by Lactuca sativa introgression lines carrying the Mo3 locus from L. virosa

et al. 2018

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The single dominant gene Mo3 from accession PIVT1398 of Lactuca virosa was shown previously to confer resistance to all tested isolates of Lettuce mosaic virus (LMV; genus Potyvirus), including mo1‐resistance‐breaking isolates. Because accession PIVT1398 is also resistant to the recently described potyvirus lettuce Italian necrotic virus (LINV), this study checked whether the Mo3 resistance locus could be involved in resistance to LINV. Introgression lines of Mo3 into two different L. sativa genetic backgrounds (Girelle and Mantilia) were resistant to isolates LMV‐9, LMV‐13 and LINV‐Fr (a French isolate of LINV), whereas Girelle and Mantilia were susceptible to these virus isolates. The resistance reaction in inoculated leaves of the introgression lines varied from absence of symptoms and absence of virus detection for LMV‐9, to hypersensitive‐like necrotic lesions and low virus titres for LINV‐Fr and large necrotic lesions and relatively abundant virus detection for LMV‐13. At the systemic level, the resistance phenotype was growth stage‐dependent for LINV‐Fr and LMV‐13. Inoculation of ≥28‐days‐old plants led to absence of symptoms and no virus with the three tested potyvirus isolates in almost all plants. Inoculation of younger plants (≤22‐days‐old) led to chlorotic/necrotic lesions on some apical leaves with low virus titre in a varying proportion of plants with LINV‐Fr and on all plants with efficient virus detection with LMV‐13. These results show that the Mo3 gene, or a tightly linked locus, in L. virosa PIVT1398 controls resistance to LINV in addition to LMV and could be used to breed L. sativa cultivars with broad‐spectrum potyvirus resistance.

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“…In recent years, several novel viruses have been characterized in lettuce crops (Ciuffo et al 2016;Svanella-Dumas et al 2018, Verbeek et al 2014, while the main viruses infecting lettuce in Europe or in Central America have been respectively reviewed in Moreno et al (2012) and Pavan et al (2008). Lettuce mosaic virus (LMV, Potyvirus) is probably the most important virus of lettuce worldwide (Pavan et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Molecular and Biological Characterization of Novel and Known Family Secoviridae Members Infecting Lettuce

et al. 2023

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High-throughput sequencing of two lettuces showing virus-like symptoms in France provided evidence of infection by members of the Secoviridae family. One plant (JG1) had a complex mixed infection that involved, among others, a novel Waikavirus (lettuce waikavirus 1) and two isolates of a sequivirus related to lettuce mottle virus (LeMoV). The second lettuce plant (JG2) was single infected by LeMoV. Complete genomic sequences were obtained for all four isolates and, in addition, near complete genome sequences were obtained for other LeMoV or LeMoV-related isolates (from French cultivated and wild lettuces, and from a Brazilian cultivated lettuce) and for two isolates of another Asteraceae-infecting sequivirus, dandelion yellow mosaic virus (DaYMV). Analysis of these genomic sequences allows to propose tentative genome organization for the various viruses and to clarify their phylogenetic relationships. Sequence and host range comparisons point to significant differences between the two sequivirus isolates identified in the JG1 plant and LeMoV isolates from France and Brazil, suggesting they belong to a novel species for which the name lettuce star mosaic virus is proposed.

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Molecular identification and biological characterization of a new potyvirus in lettuce

Cited by 8 publications

References 20 publications

Molecular and biological characterization of two potyviruses infecting lettuce in southeastern France

Molecular and biological characterization of two potyviruses infecting lettuce in southeastern France

Growth stage‐dependent resistance to the potyviruses lettuce Italian necrotic virus and Lettuce mosaic virus displayed by Lactuca sativa introgression lines carrying the Mo3 locus from L. virosa

Molecular and Biological Characterization of Novel and Known Family Secoviridae Members Infecting Lettuce

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