Complete Genome Sequences of Mycoplasma anatis, M. anseris, and M. cloacale Type Strains

Grózner, Dénes; Forró, Barbara; Sulyok, Kinga M.; Marton, Szilvia; Kreizinger, Zsuzsa; Bányai, Krisztiàn; Gyuranecz, Miklós

doi:10.1128/mra.00939-18

Cited by 4 publications

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“…In addition to the ANI and AAI assessment, the degree of total genomic DNA homology between strain 1220 T and 1340 T (GenBank accession number CP030141 [50];) was evaluated using in silico genome-to-genome comparison tool (GGDC 2.1; http://ggdc.dsmz.de), which works independently of ANI and was found to yield higher correlations with conventional DNA–DNA hybridization (DDH) [51]. Furthermore, digitally derived genome-to-genome distances showed a better correlation with 16S rRNA gene sequence distances than conventional DDH values [52].…”

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confidence: 99%

Mycoplasma anserisalpingitidis sp. nov., isolated from European domestic geese (Anser anser domesticus) with reproductive pathology

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Grózner

Gyuranecz

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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The GenBank accession numbers for the 16S rRNA gene sequence and the genome of the strain 1220 T are EU597750 and CP042295, respectively, and the GenBank accession numbers for other housekeeping genes used for the genetic characterization of each novel species described in this study are provided in the manuscript. †These authors contributed equally to this work Two supplementary figures, two supplementary tables and one supplementary document are available with the online version of this article.

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Section: Full-textmentioning

confidence: 99%

Mycoplasma anserisalpingitidis sp. nov., isolated from European domestic geese (Anser anser domesticus) with reproductive pathology

Volokhov

Grózner

Gyuranecz

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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Comparative genomic analysis of Mycoplasma anatis strains

et al. 2021

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Background The Gram-negative intracellular bacterium Mycoplasma anatis is a pathogen of respiratory infectious diseases in ducks and has caused significant economic losses in the poultry industry. Objective This study, as the first report of the structure and function of the pan-genome of Mycoplasma anatis , may provide a valuable genetic basis for many aspects of future research on the pathogens of waterfowl. Methods We sequenced the whole genomes of 15 Mycoplasma anatis isolated from ducks in China. Draft genome sequencing was carried out and whole-genome sequencing was performed by the sequencers of the PacBio Sequel and an IonTorrent Personal Genome Machine (PGM). Then the common genic elements of protein-coding genes, tRNAs, and rRNAs of Mycoplasma anatis genomes were predicted by using the pipeline Prokka v1.13.7. To investigate homologous protein clusters across Mycoplasma anatis genomes, we adopted Roary v3.13.0 to cluster orthologous genes (OGs) based on the following criteria. Results We obtained one complete genome and 14 genome sketches. Microbial mobile genetic element analysis revealed the distribution of insertion sequences (IS30, IS3, and IS1634), prophage regions, and CRISPR arrays in the genome of Mycoplasma anatis . Comparative genomic analysis decoded the genetic components and functional classification of the pan-genome of Mycoplasma anatis that comprised 646 core genes, 231 dispensable genes and among them 110 was strain-specific. Virulence-related gene profiles of Mycoplasma anatis were systematically identified, and the products of these genes included bacterial ABC transporter systems, iron transport proteins, toxins, and secretion systems. Conclusion A complete virulence-related gene profile of Mycoplasma anatis has been identified, most of the genes are highly conserved in all strains. Sequencing results are relevant to the molecular mechanisms of drug resistance, adaptive evolution of pathogens, population structure, and vaccine development. Supplementary Information The online version contains supplementary material available at 10.1007/s13258-021-01129-5.

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Characterisation of mobile genetic elements in Mycoplasma hominis with the description of ICEHo-II, a variant mycoplasma integrative and conjugative element

et al. 2020

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Background Mobile genetic elements are found in genomes throughout the microbial world, mediating genome plasticity and important prokaryotic phenotypes. Even the cell wall-less mycoplasmas, which are known to harbour a minimal set of genes, seem to accumulate mobile genetic elements. In Mycoplasma hominis, a facultative pathogen of the human urogenital tract and an inherently very heterogeneous species, four different MGE-classes had been detected until now: insertion sequence ISMhom-1, prophage MHoV-1, a tetracycline resistance mediating transposon, and ICEHo, a species-specific variant of a mycoplasma integrative and conjugative element encoding a T4SS secretion system (termed MICE). Results To characterize the prevalence of these MGEs, genomes of 23 M. hominis isolates were assembled using whole genome sequencing and bioinformatically analysed for the presence of mobile genetic elements. In addition to the previously described MGEs, a new ICEHo variant was found, which we designate ICEHo-II. Of 15 ICEHo-II genes, five are common MICE genes; eight are unique to ICEHo-II; and two represent a duplication of a gene also present in ICEHo-I. In 150 M. hominis isolates and based on a screening PCR, prevalence of ICEHo-I was 40.7%; of ICEHo-II, 28.7%; and of both elements, 15.3%. Activity of ICEHo-I and -II was demonstrated by detection of circularized extrachromosomal forms of the elements through PCR and subsequent Sanger sequencing. Conclusions Nanopore sequencing enabled the identification of mobile genetic elements and of ICEHo-II, a novel MICE element of M. hominis, whose phenotypic impact and potential impact on pathogenicity can now be elucidated.

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Complete Genome Sequences of Mycoplasma anatis, M. anseris, and M. cloacale Type Strains

Abstract: Mycoplasma anatis, M. anseris, and M. cloacale are pathogens of waterfowl. Airsacculitis, nervous disease, and reproductive disorders are the main symptoms in the affected flocks.

Cited by 4 publications

References 16 publications

Mycoplasma anserisalpingitidis sp. nov., isolated from European domestic geese (Anser anser domesticus) with reproductive pathology

Mycoplasma anserisalpingitidis sp. nov., isolated from European domestic geese (Anser anser domesticus) with reproductive pathology

Comparative genomic analysis of Mycoplasma anatis strains

Characterisation of mobile genetic elements in Mycoplasma hominis with the description of ICEHo-II, a variant mycoplasma integrative and conjugative element

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