Complete Genome Sequences of Eight Helicobacter pylori Strains with Different Virulence Factor Genotypes and Methylation Profiles, Isolated from Patients with Diverse Gastrointestinal Diseases on Okinawa Island, Japan, Determined Using PacBio Single-Molecule Real-Time Technology

Abstract

We report the complete genome sequences of eight Helicobacter pylori strains isolated from patients with gastrointestinal diseases in Okinawa, Japan. Whole-genome sequencing and DNA methylation detection were performed using the PacBio platform. De novo assembly determined a single, complete contig for each strain. Furthermore, methylation analysis identified virulence factor genotype-dependent motifs.

Sequence data for Clostridium autoethanogenum using three generations of sequencing technologies

“…This underlines the requirement and utility of hybrid approaches to the scientific community. The long-read PacBio platform was speculated to be increasingly used to produce finished microbial genome assemblies 4 , 6 , supported by several recent examples 19–23 and the utility of long-read sequencing for microbial genomes has been reviewed recently 24 . Combined developments of greater sequence output and longer reads, together with the random nature of PacBio errors have facilitated improved de novo assembly outputs.…”

Amelioration of Helicobacter pylori-Induced PUD by Probiotic Lactic Acid Bacteria

“…The genomes of various H. pylori strains have been completely sequenced so far including 26695, J99, Oki102, Oki112, Oki128, Oki154, Oki422, Oki673, Oki828, and Oki898 (Alm et al, 1999;Satou et al, 2014;Figures 67.2 and 67.3). pylori is a mesophilic Gram-negative, spiral-shaped, host-associated bacteria.…”

First Complete Genome Sequence of Clostridium sporogenes DSM 795 T , a Nontoxigenic Surrogate for Clostridium botulinum, Determined Using PacBio Single-Molecule Real-Time Technology

“…This result suggests that the number of tandem repeats is underestimated in the 10696 T sequence. The SMRT technology provides power for genome sequencing with multikilobase extra-long reads and unbiased G+C coverage ( 11 , 14 , 15 ) for assessing structural variations such as variable number tandem repeat.…”