Complete genome sequence of Trueperella pyogenes strain Arash114, isolated from the uterus of a water buffalo (Bubalus bubalis) in Iran

Abstract

Objective Trueperella pyogenes has been considered a major causative agent of metritis, abortion, and death in a broad range of domestic and wild animals, including cattle, swine, sheep, goats, camels, buffalo, deer, antelopes, reptiles, and birds. Data description Here, we report the complete chromosome sequence of Trueperella pyogenes strain Arash114, isolated from the uterus of a water buffalo (Bubalus bubalis) died due to the infection caused by this pathogen. The genome assembly comprised 2,338,282 bp, with a 59.5% GC content. Annotation of the genome showed 46 tRNA genes, 6 rRNA, 1 CRISPR and 2059 coding sequences. Also, several genes coding for antimicrobial resistance such as tetW and virulence factor including plo, nanH, nanP, cbp and 4 fimbrial proteins were found. This study will advance our knowledge regarding the metabolism, virulence factors, antibiotic resistance and evolution of Arash114 strain and serve as an appropriate template for future researches.

Comparative Genome Analysis of 19 Trueperella pyogenes Strains Originating from Different Animal Species Reveal a Genetically Diverse Open Pan-Genome

“…Next, the functional classes that were enriched with most CDS of core-genome after the function unknown category includes carbohydrate metabolism and transport (139), translation (135), inorganic ion transport and metabolism (120), transcription (115), amino acid metabolism and transport (113), and energy production and conversion (93), replication and repair (85), defence mechanism (31), among others (Table S6a,b; Figure 4A). On the other hand, 31.29% CDS of strain-specific genes subset were assigned into functional categories of replication and repair (23), function unknown (16), defence mechanism (12), and transcription (10), among others (Table S6a,b; Figure 4B). The functional analysis underlines the metabolic versatility of T. pyogenes, as it encompasses most of the basic categories of gene ontology [64].…”

“…In this comparative genomics study of T. pyogenes strains from all over the world, we have also included the first Indian draft genome sequence of T. pyogenes species (Bu5 strain), which was isolated from wound infections of water buffalo (Bubalus bubalis). Notably, infections due to T. pyogenes in water buffalo have been reported from Mediterranean and Middle East countries [16][17][18]. In India, cases of T. pyogenes infection have been reported from small ruminants [19]; however, reports from buffaloes in India have been elusive.…”

Construction of live-attenuated Trueperella pyogenes by antibiotic treatment and sequential passage: methods for vaccine development

“…In this study, T. pyogenes , number PTCC1925 (Persian Type Culture Collection), isolated from uterus of a water bufalo ( Bubalus bubalis ) in Iran was selected (Ashrafi Tamai et al 2021a ). Lyophilized T. pyogenes was cultured in Blood Agar with 7% sheep blood and incubated at 37 °C with 5% CO2 for 48 h, pure colonies with beta hemolysis were selected.…”