Complete Genome Sequence of the Lytic Bacteriophage Phab24, Which Infects Clinical Strains of the Nosocomial Pathogen Acinetobacter baumannii

Loh, Belinda; Wang, Xiaoqing; Hua, Xiaoting; Chook, Hou Wei; Ma, Liang; Zhang, Liwei; Manohar, Prasanth; Jin, Yijie; Leptihn, Sebastian

doi:10.1128/mra.00669-21

Cited by 5 publications

(5 citation statements)

References 15 publications

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“…We isolated several phages that are able to infect both XH198 and ATCC17978. To focus on one particular virus-host system, we selected a phage that we named Phab24, for Ph age A cinetobacter b aumannii number 24 [ 18 ] ( Figure 1 (A)). Whole genome analysis using the programme PhageAI ( https://phage.ai/ ) revealed that bacteriophage Phab24 is virulent (lytic) with a 93.76% prediction confidence [ 19 ].…”

Section: Resultsmentioning

confidence: 99%

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Colistin-phage combinations decrease antibiotic resistance in Acinetobacter baumannii via changes in envelope architecture

Wang

Loh

Altamirano

et al. 2021

Emerging Microbes & Infections

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Multidrug-resistant bacterial infections are becoming increasingly common, with only few last-resort antibiotics such as colistin available for clinical therapy. An alternative therapeutic strategy gaining momentum is phage therapy, which has the advantage of not being affected by bacterial resistance to antibiotics. However, a major challenge in phage therapy is the rapid emergence of phage-resistant bacteria. In this work, our main aim was to understand the mechanisms of phage-resistance used by the top priority pathogen Acinetobacter baumannii . We isolated the novel phage Phab24, capable of infecting colistin-sensitive and -resistant strains of A. baumannii . After co-incubating Phab24 with its hosts, we obtained phage-resistant mutants which were characterized on both genotypic and phenotypic levels. Using whole genome sequencing, we identified phage-resistant strains that displayed mutations in genes that alter the architecture of the bacterial envelope at two levels: the capsule and the outer membrane. Using an adsorption assay, we confirmed that phage Phab24 uses the bacterial capsule as its primary receptor, with the outer membrane possibly serving as the secondary receptor. Interestingly, the phage-resistant isolates were less virulent compared to the parental strains in a Galleria mellonella infection model. Most importantly, we observed that phage-resistant bacteria that evolved in the absence of antibiotics exhibited an increased sensitivity to colistin, even though the antibiotic resistance mechanism per se remained unaltered. This increase in antibiotic sensitivity is a direct consequence of the phage-resistance mechanism, and could potentially be exploited in the clinical setting.

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Section: Resultsmentioning

confidence: 99%

“…Isolation, purification and the genome of phage Phab24 are described elsewhere [ 18 ]. GenBank accession number for Phab24 genome: MZ477002.…”

Section: Methodsmentioning

confidence: 99%

Colistin-phage combinations decrease antibiotic resistance in Acinetobacter baumannii via changes in envelope architecture

Wang

Loh

Altamirano

et al. 2021

Emerging Microbes & Infections

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“…According to the phylogenetic analysis performed using major capsid protein sequences (Figure 1), lytic TSP-carrying Acinetobacter phages belonging to unclassified Caudoviricetes include two distantly related clusters, conventionally designated as cluster 13 (phages AM24, Bestia, Herod, Mithridates, P577, and YMC13/03/R2096) and cluster 14 (phages BS46, Phab24, TaPaz, and vB_AbaM_B9). Both clusters combine phages with a typical myovirus morphology [28,33,71,111].…”

Section: Specificity Of the Lytic Vequintavirinae Phage Abph49 (Clust...mentioning

confidence: 99%

Lytic Capsule-Specific Acinetobacter Bacteriophages Encoding Polysaccharide-Degrading Enzymes

Evseev,

Sukhova,

Tkachenko

et al. 2024

Viruses

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The genus Acinetobacter comprises both environmental and clinically relevant species associated with hospital-acquired infections. Among them, Acinetobacter baumannii is a critical priority bacterial pathogen, for which the research and development of new strategies for antimicrobial treatment are urgently needed. Acinetobacter spp. produce a variety of structurally diverse capsular polysaccharides (CPSs), which surround the bacterial cells with a thick protective layer. These surface structures are primary receptors for capsule-specific bacteriophages, that is, phages carrying tailspikes with CPS-depolymerizing/modifying activities. Phage tailspike proteins (TSPs) exhibit hydrolase, lyase, or esterase activities toward the corresponding CPSs of a certain structure. In this study, the data on all lytic capsule-specific phages infecting Acinetobacter spp. with genomes deposited in the NCBI GenBank database by January 2024 were summarized. Among the 149 identified TSPs encoded in the genomes of 143 phages, the capsular specificity (K specificity) of 46 proteins has been experimentally determined or predicted previously. The specificity of 63 TSPs toward CPSs, produced by various Acinetobacter K types, was predicted in this study using a bioinformatic analysis. A comprehensive phylogenetic analysis confirmed the prediction and revealed the possibility of the genetic exchange of gene regions corresponding to the CPS-recognizing/degrading parts of different TSPs between morphologically and taxonomically distant groups of capsule-specific Acinetobacter phages.

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“…Phages were obtained from clear single plaques and grown in the presence of the bacterial host in LB overnight. Bacterial cells were removed by centrifugation, and the supernatant was filtered through a 0.22-μm membrane ( 6 ). Nucleic acids were extracted using the Biomed virus rapid DNA/RNA kit (Beijing, China) according to the manufacturer’s instructions.…”

Section: Announcementmentioning

confidence: 99%

Complete Genome Sequences of Bacteriophages Kaya, Guyu, Kopi, and TehO, Which Target Clinical Strains of Pseudomonas aeruginosa

Loh

Wang

Hua

et al. 2021

Microbiol Resour Announc

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Complete Genome Sequence of the Lytic Bacteriophage Phab24, Which Infects Clinical Strains of the Nosocomial Pathogen Acinetobacter baumannii

Cited by 5 publications

References 15 publications

Colistin-phage combinations decrease antibiotic resistance in Acinetobacter baumannii via changes in envelope architecture

Colistin-phage combinations decrease antibiotic resistance in Acinetobacter baumannii via changes in envelope architecture

Lytic Capsule-Specific Acinetobacter Bacteriophages Encoding Polysaccharide-Degrading Enzymes

Complete Genome Sequences of Bacteriophages Kaya, Guyu, Kopi, and TehO, Which Target Clinical Strains of Pseudomonas aeruginosa

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