Complete Genome Sequences of Bacteriophages Kaya, Guyu, Kopi, and TehO, Which Target Clinical Strains of Pseudomonas aeruginosa

Loh, Belinda; Wang, Xiaoqing; Hua, Xiaoting; Luo, Junhan; Wen, Tanye; Zhang, Liwei; Ma, Liang; Manohar, Prasanth; Nachimuthu, Ramesh; Grainge, Ian; Yu, Yunsong; Leptihn, Sebastian

doi:10.1128/mra.01043-21

Cited by 6 publications

(5 citation statements)

References 17 publications

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“…S1). Standard nucleotide BLAST demonstrated that phipa2, phipa4, and phipa10 closely resemble that of the P. aeruginosa phage vB_Pae_QDWS (coverage 94% and identity 96.81%) ( 22 ), phage Kopi (coverage 93% and identity 96.60%) ( 23 ), and phage YS35 (coverage 96% and identity 96.76%) ( 24 ), respectively. The schematic maps of the phage genomes are listed in Fig.…”

Section: Resultsmentioning

confidence: 99%

Characterization of Phage Resistance and Their Impacts on Bacterial Fitness in Pseudomonas aeruginosa

Zeng

Wang

et al. 2022

Microbiol Spectr

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Section: Resultsmentioning

confidence: 99%

Characterization of Phage Resistance and Their Impacts on Bacterial Fitness in Pseudomonas aeruginosa

Zeng

Wang

et al. 2022

Microbiol Spectr

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“…From the previous studies, the isolate was found to be multi-drug resistant (MDR) and cause bacteremia in mice [ 16 ]. For the treatment, two previously characterized bacteriophages were used which include Pseudomonas phage AP025 and Pseudomonas phage AP006 [ 22 ]. The characterized Pseudomonas phage AP025 ( Myoviridae ) had infectivity of 39% and Pseudomonas phage AP006 ( Siphoviridae ) with 30% infectivity against 51 P. aeruginosa tested.…”

Section: Methodsmentioning

confidence: 99%

“…Phages for therapy should be strictly virulent (also known as lytic), as studies have shown that prophages (genome-embedded phages with a lysogenic cycle) can alter the characteristics of a host and in some cases, increase virulence, not only in P. aeruginosa but also in other pathogens [ 19 – 21 ]. Many Pseudomonas phages with potential for clinical use have been reported [ 22 – 24 ], however, the majority of previously published studies on phage therapy focused mainly on wound infections [ 25 , 26 ]. Our study describes the use of phages in bloodstream infections caused by the pathogen.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial efficacy of lytic phages against multidrug-resistant Pseudomonas aeruginosa infections in bacteraemia mice models

et al. 2022

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Background Pseudomonas aeruginosa is an opportunistic pathogen that can cause a variety of infections in humans, such as burn wound infections and infections of the lungs, the bloodstream and surgical site infections. Nosocomial spread is often concurrent with high degrees of antibiotic resistance. Such resistant strains are difficult to treat, and in some cases, even reserved antibiotics are ineffective. A particularly promising therapy to combat infections of resistant bacteria is the deployment of bacteriophages, known as phage therapy. In this work, we evaluated the in vivo efficacy of two Pseudomonas phages in bacteremia mice models. For this study, non-neutropenic mice (BalB/C) were infected with P. aeruginosa AB030 strain and treated using two bacteriophages, AP025 and AP006. Results The results showed that a single dose of phages at higher concentrations, bacteria: phage at 1:10 and 1:100 were effective in eliminating the bloodstream infection and achieving 100% mice survival. Conclusion This study highlights the efficacy of using a single dose of phages to restore mice from bacteremia.

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“…no. MZ927746) with 40% query coverage sharing 93.74% to 96.68% identity ( Clark et al, 2019 ; Loh et al, 2021 ). Comparative genomics of NR08 phage with ten Xoophages reported by Kovács et al (2019) (Accession no.…”

Section: Discussionmentioning

confidence: 99%

Isolation of bacteriophages infecting Xanthomonas oryzae pv. oryzae and genomic characterization of novel phage vB_XooS_NR08 for biocontrol of bacterial leaf blight of rice

Jain

Kumar²,

Jain³

et al. 2023

Front. Microbiol.

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Bacterial leaf blight (BLB) disease of rice caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most destructive diseases worldwide in rice-growing regions. The Ineffectiveness of chemicals in disease management has increased the interest in phage therapy. In this study, we isolated 19 bacteriophages, infecting Xoo, from a rice field, which belonged to phage families Siphoviridae, Myoviridae, and Podoviridae on the basis of electron microscopy. Among 19 phages, Phage vB_XooS_NR08, a member of the Siphoviridae family, expressed antibacterial activity against all Xoo strains tested and did not lyse X. campestris and other unrelated bacterial hosts. Phage NR08 showed more than 80% viability at a temperature range of 4°C–40°C, pH range of 5–9, and direct exposure to sunlight for 2 h, whereas UV light and chemical agents were highly detrimental. In a one-step growth curve, NR08 has a 40-min latent period, followed by a 30-min burst period with a burst size of 250 particle/bacterium. The genome of NR08 is double-stranded DNA, linear having a size of 98,812 bp with a G + C content of 52.9%. Annotation of the whole-genome sequence indicated that NR08 encodes 142 putative open reading frames (ORFs), including one ORF for tRNA, namely, trna1-GlnTTG. Comparative genome analysis of NR08 showed that it shares maximum similarity with Pseudomonas phage PaMx42 (40% query coverage, 95.39% identity, and acc. Length 43,225) and Xanthomonas phage Samson (40% query coverage, 96.68% identity, and acc. Length 43,314). The average alignment percentage (AP) of NR08 with other Xoophages was only 0.32 to 1.25 since the genome of NR08 (98.8 kb) is almost double of most of the previously reported Xoophages (43–47 kb), thus indicating NR08 a novel Xoophage. In in vitro bacterial challenge assay, NR08 showed bacteriostasis up to 24 h and a 99.95% reduction in bacterial growth in 48 h. In rice pot efficacy trials, single-dose treatment of NR08 showed a significant reduction in disease up to 90.23% and 79.27% on 7 and 21 dpi, respectively. However, treatment using 2% skim milk-supplemented phage preparation was significantly less effective as compared to the neat phage preparation. In summary, this study characterized a novel Xoophage having the potential as a biocontrol agent in the mitigation of BLB in rice.

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Complete Genome Sequences of Bacteriophages Kaya, Guyu, Kopi, and TehO, Which Target Clinical Strains of Pseudomonas aeruginosa

Abstract: Pseudomonas aeruginosa is a major public health concern, as drug-resistant strains increase mortality in hospital-acquired infections. We report the isolation and complete genome sequences of four lytic bacteriophages that target clinical multidrug-resistant P. aeruginosa strains.

Cited by 6 publications

References 17 publications

Characterization of Phage Resistance and Their Impacts on Bacterial Fitness in Pseudomonas aeruginosa

Characterization of Phage Resistance and Their Impacts on Bacterial Fitness in Pseudomonas aeruginosa

Antibacterial efficacy of lytic phages against multidrug-resistant Pseudomonas aeruginosa infections in bacteraemia mice models

Isolation of bacteriophages infecting Xanthomonas oryzae pv. oryzae and genomic characterization of novel phage vB_XooS_NR08 for biocontrol of bacterial leaf blight of rice

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