Complete Genome Sequence of the Type Strain of the Acetogenic Bacterium Moorella thermoacetica DSM 521 ^T

Abstract

Here we report the closed genome sequence of the type strain Moorella thermoacetica DSM 521T, an acetogenic bacterium, which is able to grow autotrophically on H2 + CO2 and/or CO, using the Wood-Ljungdahl pathway. The genome consists of a circular chromosome (2.53 Mb).

Using gas mixtures of CO, CO2 and H2 as microbial substrates: the do's and don'ts of successful technology transfer from laboratory to production scale

“…A. woodii was isolated in 1977, when the very first described autotrophic acetogen, that is C. aceticum was still considered to be lost (Balch et al ., ). C. aceticum was only rediscovered in 1980 and later completely sequenced (Braun et al ., ; Poehlein et al ., ,b,c). A. woodii does not use CO as a substrate, but is very efficient in fermenting CO 2 /H 2 gas mixtures.…”

“…Andreesen et al, 1973;Daniel et al, 1990;Parekh and Cheryan, 1991;G€ oßner et al, 1999;Bengelsdorf et al, 2015a;Poehlein et al, 2015a) …”

Two propanediol utilization-like proteins of Moorella thermoacetica with phosphotransacetylase activity

“…Therefore, the therein used gene locus tags moth_1181 and moth_0864 which corresponding gene products were annotated as PDUL1 and PDUL2 were also used for the strain M. thermoacetica DSM 521 used in this study. Its genome was recently published by Poehlein et al (2015) simultaneously to the genome of M. thermoacetica DSM 2955 . Our sequencing data showed identical PDUL2 genes (moth_0864 and MOTHE_c08100) in M. thermoacetica DSM 521 (MOTHE_c11560) compared with M. thermoacetica ATCC 39073 (moth_1181) and, furthermore, proved a C to T mutation at position 104 of the PDUL1 gene in DSM 521 resulting into an amino acid exchange from threonine to isoleucine.…”

Genome-Based Comparison of All Species of the Genus Moorella, and Status of the Species Moorella thermoacetica and Moorella thermoautotrophica

“…The type strain DSM 521 T and the strain ATCC 39073 have primarily served to elucidate the primary metabolism of M. thermoacetica (synonym C. thermoaceticum): they were used in experiments to study carbohydrate utilization (Andreesen et al, 1973), the acetate kinase (Schaupp and Ljungdahl, 1974), cytochromes and menaquinones (Gottwald et al, 1975), the formate dehydrogenase (Ljungdahl and Andreesen, 1977), and the utilization of CO (Diekert and Thauer, 1978). The genome of the non-type strain ATCC 39073 was sequenced in 2008 (Pierce et al, 2008) and the genome sequence of the type strain DSM 521 T followed in 2015 (Poehlein et al, 2015). A spore sample of the original M. thermoacetica strain isolated in 1942 was deposited by Kerby and Zeikus (1983) as a second representative of the type strain (DSM 2955 T ) in the DSMZ (Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Brunswick, Germany).…”

“…We sequenced the genome of those two strains as well as the genome of DSM 7417 and nine other M. thermoacetica strains, thereby considerably adding to the genomic information of this group of bacteria. We compared the genomes of the strains with the genome of the M. thermoacetica strain ATCC 39073 (Pierce et al, 2008) and the type strains DSM 2955 T (Bengelsdorf et al, 2015) and DSM 521 T (Poehlein et al, 2015). In addition, we performed genome comparison with all other genomes of the genus Moorella.…”