Complete Genome Sequence of the Metabolically Versatile Halophilic Archaeon Haloferax mediterranei, a Poly(3-Hydroxybutyrate-
            <i>co</i>
            -3-Hydroxyvalerate) Producer

Han, Jing; Zhang, Fan; Hou, Jing; Liu, Xiaoqing; Li, Ming; Liu, Hailong; Cai, Lei; Zhang, Bing; Chen, Yaping; Zhou, Jian; Hu, Songnian; Xiang, Hua

doi:10.1128/jb.00880-12

Cited by 78 publications

(76 citation statements)

References 20 publications

(12 reference statements)

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“…mediteranneimediated PHA production has been further optimized by researchers all around the world. Whereas some of these groups focused on the application of inexpensive carbon feedstocks to safe substrate costs, such as whey permeate from dairy industry [12,13,48], rice-based ethanol stillage [49], extruded rice bran [50], enzymatically extruded starch [51], crude glycerol phase [28], olive mill waste water [52], vinasse [53], etc., others clarified the enzymatic and genetic background of PHA-synthesis [54][55][56][57] and in vitro degradation [58] by this organisms. Mathematic models of PHA-production [59], and kinetic studies of PHA and by-product synthesis and degradation [60] were reported for Hfx.…”

Section: Discussionmentioning

confidence: 99%

Production of Polyhydroxyalkanoate (PHA) Biopolyesters by Extremophiles?

Koller¹

2017

MOJPS

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The article reviews the current state of knowledge of the production of polyhydroxyalkanoate (PHA) biopolyesters under extreme environmental conditions.Although PHA production by extremophiles is not realized yet at industrial scale, significant PHA accumulation under high salinity or extreme pH-or temperature conditions was reported for diverse representatives of the microbial domains of both Archaea and Bacteria. Several mechanisms were proposed to explain the mechanistic role of PHA and their monomers as microbial cell-and enzyme protective chaperons and the factors boosting PHA biosynthesis under environmental stress conditions. The potential of selected extremophile strains, isolated from extreme environments like glaciers, hot springs, saline brines, or from habitats highly polluted with heavy metals or solvents, for efficient future PHA production on an industrially relevant scale is assessed based on the basic data available in the scientific literature. The article reveals that, beside the needed optimization of other cost-decisive factors like inexpensive raw materials or efficient downstream processing, the application of extremophile production strains can drastically safe energy costs, are easily accessible towards long-term cultivation in chemostat processes, and therefore might pave the way towards cost-efficient PHA production, even combined with safe disposal of industrial waste streams. However, further challenges have still to be overcome in terms of strain improvement and process engineering aspects.

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Section: Discussionmentioning

confidence: 99%

Production of Polyhydroxyalkanoate (PHA) Biopolyesters by Extremophiles?

Koller¹

2017

MOJPS

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“…The outcomes of published studies evidence the high stability of this organism during long-term continuous cultivation and the convenient recovery of accumulated PHA-granules without the need of organic extraction solvents 33 . In addition, similar to Cupriavidus necator, the best investigated PHA producer 34 , the entire genome sequence of H. mediterranei is deciphered and reported 35 .…”

Section: Pha and Eps From H Mediterraneimentioning

confidence: 99%

Study on the Production and Re-use of Poly(3-hydroxybutyrate-co-3- hydroxyvalerate) and Extracellular Polysaccharide by the Archaeon Haloferax mediterranei Strain DSM 1411

Koller

Chiellini

Braunegg

2015

Chem.Biochem.Eng.Q.

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The halobacterium Haloferax mediterranei was used to study the production of two types of biopolymers: The biopolyester poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was accumulated as intracellular granules, whereas an extracelluar polysaccharide was excreted in parallel to biopolyester synthesis.After production, microbial re-use and degradation of these polymers under different conditions were investigated to assess the requirements for handling the product-rich fermentation broth prior to the downstream processing for product recovery. Degradation kinetics of the polymers and the impact of different storage conditions on molar mass of PHBV were studied.It turned out that the biotechnological fermentation process can be run without any sterility precautions. No major product losses were observed without pasteurization of fermentation broth after the stop of fermentation. In addition, neither PHBV nor EPS are re-utilized by the cells for biomass formation even if the culture is maintained under conditions of carbon starvation for an extended time.

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“…Currently, progress have been made in understanding the genomes of other species such as Hfx. mediterranei ATCC33500 (Han et al, 2012), Hfx. gibbonsii strain ARA 6 (Pinto, D'Alincourt Carvalho-Assef, Vieira, Clementino & Albano, 2015) or Hfx.…”

Section: Introductionmentioning

confidence: 99%

Anaerobic Metabolism in Haloferax Genus

Torregrosa-Crespo

Martínez‐Espinosa

Esclapez

et al. 2016

Advances in Microbial Physiology

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A number of species of Haloferax genus (halophilic Archaea) are able to grow micro aerobically or even anaerobically using different alternative electron acceptors such as fumarate, nitrate, chlorate, dimethyl sulphoxide, sulphide and/or trimethylamine. This metabolic capability is also shown by other species of the Halobacteriaceae and Haloferacaceae families (Archaea domain) and it has been mainly tested by physiological studies where cells growth is observed under anaerobic conditions in the presence of the mentioned compounds. This work summarises the main reported features on anaerobic metabolism in the Haloferax, one of the better described haloarchaeal genus with significant potential uses in Biotechnology and Bioremediation. Special attention has been paid to denitrification, also called nitrate respiration. This pathway has been studied so far from KEY WORDSHaloarchaea, denitrification, anaerobic metabolism, Nox emissions, bioremediation.

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Complete Genome Sequence of the Metabolically Versatile Halophilic Archaeon Haloferax mediterranei, a Poly(3-Hydroxybutyrate- co -3-Hydroxyvalerate) Producer

Cited by 78 publications

References 20 publications

Production of Polyhydroxyalkanoate (PHA) Biopolyesters by Extremophiles?

Production of Polyhydroxyalkanoate (PHA) Biopolyesters by Extremophiles?

Study on the Production and Re-use of Poly(3-hydroxybutyrate-co-3- hydroxyvalerate) and Extracellular Polysaccharide by the Archaeon Haloferax mediterranei Strain DSM 1411

Anaerobic Metabolism in Haloferax Genus

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