Complete Genome Sequence of Sphingobacterium sp. Strain CZ-2T Isolated from Tobacco Leaves Infected with Wildfire Disease

Abstract

Background Sphingobacterium is a class of Gram-negative, non-fermentative bacilii, and rarely involved in human infections. It is characterized by a large number of cellular membrane sphingophospolipids. Due to its wide ecological distribution and oil degradation ability, environmental microbiologists have paid much attention to it. Results A novel gram-negative bacterium, designated CZ-2 T , was isolated from a sample of tobacco leaves infected with wildfire disease in Guiyang County, Chenzhou City, Hunan Province, China, and its phylogenetic position was investigated by GC content determination, PCR amplification, sequencing and phylogenetic analysis. Growth occurred on TGY medium at 30 °C and pH 7.0. The GC content of the DNA of strain CZ-2 T is 40.68 mol%. Genome relatedness, rDNA phylogeny and chemotaxonomic characteristics all indicate that strain CZ-2 T represents a novel species of the genus Sphingobacterium . We propose the name Sphingobacterium. tobaci sp. nov., with CZ-2 T as the type strain. Third-generation sequencing (TGS) and next-generation sequencing (NGS) were used to derive a finished genome sequence for strain CZ-2 T , consisting of a circular chromosome 3,925,977 bp in size. The genome of strain CZ-2 T features 3,462 protein-encoding and 50 tRNA-encoding genes. Unigenes were annotated by matching against Clusters of Orthologous Groups of proteins (COG; 2,021 genes), Gene Ontology (GO; 1,952 genes) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases (1,380 genes). Comparison of the predicted proteome of CZ-2 T with those of other Sphingobacterium identified 677 species-specific proteins that may contribute to the adaptation of CZ-2 T to its native environment. Conclusions As the first report of a Sphingobacterium genome sequenced by NGS and TGS, our work will serve as a useful reference for subsequent sequencing and mapping efforts for additional strains and species within this genus.

Isolation and identification of pathogenic bacteria in the digestive microbiota of some aquatic insect

“…Sphingobacterium sp. isolated from Helophorus aquaticus and Laccobius sulcatulus insect species has biotechnological importance in addition to being a bacterium that may be a rare infectious vector (Cai et al 2019).…”