Complete Genome Sequence of Pantoea agglomerans ASB05 Using Illumina and PacBio Sequencing

Lee, Sang In; Tran, Thao Dang-Hien; Huynh, Steven; Parker, Craig T.; Hnasko, Robert; McGarvey, Jeffery A.

doi:10.1128/mra.00501-21

Microbiol Resour Announc

2021

DOI: 10.1128/mra.00501-21

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Complete Genome Sequence of Pantoea agglomerans ASB05 Using Illumina and PacBio Sequencing

Sang In Lee

Thao Dang-Hien Tran

Steven Huynh

et al.

Abstract: We present the complete genome sequence of Pantoea agglomerans ASB05 and three associated plasmids, generated using a combination of the Illumina and PacBio platforms. P. agglomerans ASB05 was isolated from fresh cherries purchased in Albany, California, in 2016.

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Cited by 3 publications

References 19 publications

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Use of Pantoea agglomerans ASB05 as a biocontrol agent to inhibit the growth of Salmonella enterica on intact cantaloupe melons

Lee,

Tran,

Hnasko

et al. 2023

Journal of Applied Microbiology

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Aims To identify biocontrol agents to prevent the growth of Salmonella enterica on cantaloupe melons during the pre- and post-harvest periods. Methods and results We created a produce-associated bacterial library containing 8,736 isolates and screened it using an in vitro fluorescence inhibition assay to identify bacteria that inhibit the growth of S. enterica. One isolate, Pantoea agglomerans ASB05, was able to grow, persist and inhibit the growth of S. enterica on intact cantaloupe melons under simulated pre- and post-harvest conditions. We also demonstrated that the growth inhibition of S. enterica by P. agglomerans ASB05 was due to the production of a phenazine type antibiotic. Conclusions P. agglomerans ASB05 is an effective biocontrol agent for the prevention of S. enterica growth on intact cantaloupe melons in both the pre- and post-harvest environments.

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Use of Pantoea agglomerans ASB05 as a biocontrol agent to inhibit the growth of Salmonella enterica on intact cantaloupe melons

Lee,

Tran,

Hnasko

et al. 2023

Journal of Applied Microbiology

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Interaction of bacteriophage P1 with an epiphytic Pantoea agglomerans strain—the role of the interplay between various mobilome elements

Giermasińska-Buczek,

Gawor,

Stefańczyk

et al. 2024

Front. Microbiol.

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P1 is a model, temperate bacteriophage of the 94 kb genome. It can lysogenize representatives of the Enterobacterales order. In lysogens, it is maintained as a plasmid. We tested P1 interactions with the biocontrol P. agglomerans L15 strain to explore the utility of P1 in P. agglomerans genome engineering. A P1 derivative carrying the Tn9 (cmR) transposon could transfer a plasmid from Escherichia coli to the L15 cells. The L15 cells infected with this derivative formed chloramphenicol-resistant colonies. They could grow in a liquid medium with chloramphenicol after adaptation and did not contain prophage P1 but the chromosomally inserted cmR marker of P1 Tn9 (cat). The insertions were accompanied by various rearrangements upstream of the Tn9 cat gene promoter and the loss of IS1 (IS1L) from the corresponding region. Sequence analysis of the L15 strain genome revealed a chromosome and three plasmids of 0.58, 0.18, and 0.07 Mb. The largest and the smallest plasmid appeared to encode partition and replication incompatibility determinants similar to those of prophage P1, respectively. In the L15 derivatives cured of the largest plasmid, P1 with Tn9 could not replace the smallest plasmid even if selected. However, it could replace the smallest and the largest plasmid of L15 if its Tn9 IS1L sequence driving the Tn9 mobility was inactivated or if it was enriched with an immobile kanamycin resistance marker. Moreover, it could develop lytically in the L15 derivatives cured of both these plasmids. Clearly, under conditions of selection for P1, the mobility of the P1 selective marker determines whether or not the incoming P1 can outcompete the incompatible L15 resident plasmids. Our results demonstrate that P. agglomerans can serve as a host for bacteriophage P1 and can be engineered with the help of this phage. They also provide an example of how antibiotics can modify the outcome of horizontal gene transfer in natural environments. Numerous plasmids of Pantoea strains appear to contain determinants of replication or partition incompatibility with P1. Therefore, P1 with an immobile selective marker may be a tool of choice in curing these strains from the respective plasmids to facilitate their functional analysis.

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Identification and Characterization of a Potential Antibiotic Producing Strain of Pantoea ananatis

LaGier¹,

McDaniel²,

Ragner³

et al. 2022

J. Genomics

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Antibiotic resistance continues to be a significant public health challenge. Soil bacteria represent a potential source of yet to be discovered antimicrobials. The screening of Iowa (United States) soils yielded the identification of a strain of Pantoea ananatis (MMB-1), which displayed an antimicrobial-producing phenotype against a bacterium ( Bacillus subtilis ) representative of Gram-positive bacteria. Crude, organic, extracts of MMB-1 retained the anti-microbial activity. The draft genome of strain MMB-1 contains a total of 4,634,340 bp, and 4,624 protein-encoding genes. Consistent with phenotypic observation, the genome of MMB-1 encodes for a number of putative secondary metabolite biosynthetic gene clusters, including those known to be involved in the production of the antibiotics lankacidin C and bottromycin. This study increases our overall understanding of Panteoa as a group, and is also consistent with the notion that members of this genus have significant potential as useful natural product producers.

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Complete Genome Sequence of Pantoea agglomerans ASB05 Using Illumina and PacBio Sequencing

Abstract: We present the complete genome sequence of Pantoea agglomerans ASB05 and three associated plasmids, generated using a combination of the Illumina and PacBio platforms. P. agglomerans ASB05 was isolated from fresh cherries purchased in Albany, California, in 2016.

Cited by 3 publications

References 19 publications

Use of Pantoea agglomerans ASB05 as a biocontrol agent to inhibit the growth of Salmonella enterica on intact cantaloupe melons

Use of Pantoea agglomerans ASB05 as a biocontrol agent to inhibit the growth of Salmonella enterica on intact cantaloupe melons

Interaction of bacteriophage P1 with an epiphytic Pantoea agglomerans strain—the role of the interplay between various mobilome elements

Identification and Characterization of a Potential Antibiotic Producing Strain of Pantoea ananatis

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