Complete Genome Sequence of Mycoplasma synoviae Strain WVU 1853
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May, Meghan; Kutish, G. F.; Barbet, Anthony F.; Michaels, Dina L.; Brown, Daniel R.

doi:10.1128/genomea.00563-15

Cited by 7 publications

(8 citation statements)

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“…Among the CRISPR- associated genes, csn1 appeared intact in MS53, but was present as a pseudogene in WVU-1853 and MS-H. In WVU-1853, csn1 was disrupted by a single nucleotide deletion resulting in a frameshift [ 11 ]. In MS-H, csn1 was disrupted by an 11 nucleotide insertion.…”

Section: Resultsmentioning

confidence: 99%

“…Such knowledge would help to define molecular markers for tracking MS-H in vaccinated flocks and assessing any variation in the level of cross-protection against local strains of M. synoviae . The sequences of the M. synoviae field strain MS53, isolated around 2003 from a broiler flock in Brazil [ 10 ], and the type strain WVU-1853, isolated in 1957 from a hock joint of chicken in the USA [ 11 ], are the only two complete genomes determined thus far for this species. This is possibly due to difficulties experienced in assembling next generation sequencing (NGS) data for M. synoviae , which contains large, low complexity, repeat-rich regions.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genome analysis of Mycoplasma synoviae strain MS-H, the most common M. synoviae strain with a worldwide distribution

et al. 2018

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BackgroundThe bacterial pathogen Mycoplasma synoviae can cause subclinical respiratory disease, synovitis, airsacculitis and reproductive tract disease in poultry and is a major cause of economic loss worldwide. The M. synoviae strain MS-H was developed by chemical mutagenesis of an Australian isolate and has been used as a live attenuated vaccine in many countries over the past two decades. As a result it may now be the most prevalent strain of M. synoviae globally. Differentiation of the MS-H vaccine from local field strains is important for epidemiological investigations and is often required for registration of the vaccine.ResultsThe complete genomic sequence of the MS-H strain was determined using a combination of Illumina and Nanopore methods and compared to WVU-1853, the M. synoviae type strain isolated in the USA 30 years before the parent strain of MS-H, and MS53, a more recent isolate from Brazil. The vaccine strain genome had a slightly larger number of pseudogenes than the two other strains and contained a unique 55 kb chromosomal inversion partially affecting a putative genomic island. Variations in gene content were also noted, including a deoxyribose-phosphate aldolase (deoC) fragment and an ATP-dependent DNA helicase gene found only in MS-H. Some of these sequences may have been acquired horizontally from other avian mycoplasma species.ConclusionsMS-H was somewhat more similar to WVU-1853 than to MS53. The genome sequence of MS-H will enable identification of vaccine-specific genetic markers for use as diagnostic and epidemiological tools to better control M. synoviae.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4501-8) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genome analysis of Mycoplasma synoviae strain MS-H, the most common M. synoviae strain with a worldwide distribution

et al. 2018

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“…Until now, the complete genomes of six strains of M. synoviae from four continents have been published, which can provide a preliminary understanding of the similarities and differences of different M. synoviae strains from the same and different geographic locations. ATCC 25204 (GenBank accession No: NZ_CP011096.1) and NCTC10124 (GenBank accession No: NZ_LS991953.1) are from the same type strain WVU1583, which was isolated from a chicken joint in West Virginia, USA (6,37). ATCC 25204 is stored in the American Type Culture Collection, while NCTC10124 is preserved in the Wellcome Trust Sanger Institute, United Kingdom.…”

Section: Discussionmentioning

confidence: 99%

Comparative Genomics of Mycoplasma synoviae and New Targets for Molecular Diagnostics

Chen

et al. 2021

Front. Vet. Sci.

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Mycoplasma synoviae is an important pathogen of poultry, causing significant economic losses in this industry. Analysis of the unique genes and shared genes among different M. synoviae strains and among related species is helpful for studying the molecular pathogenesis of M. synoviae and provides valuable molecular diagnostic targets to facilitate the identification of M. synoviae species. We selected a total of 46 strains, including six M. synoviae strains, from 25 major animal (including avian) Mycoplasma species/subspecies that had complete genome sequences and annotation information published in GenBank, and used them for comparative genomic analysis. After analysis, 16 common genes were found in the 46 strains. Thirteen single-copy core genes and the 16s rRNA genes were used for genetic evolutionary analysis. M. synoviae was found to have a distant evolutionary relationship not only with other arthritis-causing mycoplasmas, but also with another major avian pathogen, Mycoplasma gallisepticum, that shares the major virulence factor vlhA with M. synoviae. Subsequently, six unique coding genes were identified as shared among these M. synoviae strains that are absent in other species with published genome sequences. Two of the genes were found to be located in the genetically stable regions of the genomes of M. synoviae and were determined to be present in all M. synoviae isolated strains (n = 20) and M. synoviae-positive clinical samples (n = 48) preserved in our laboratory. These two genes were used as molecular diagnostic targets for which SYBR green quantitative PCR detection methods were designed. The two quantitative PCR methods exhibited good reproducibility and high specificity when tested on positive plasmid controls and genomic DNA extracted from different M. synoviae strains, other major avian pathogenic bacteria/mycoplasmas, and low pathogenic Mycoplasma species. The detection limit for the two genes was 10 copies or less per reaction. The clinical sensitivity and specificity of the quantitative PCR methods were both 100% based on testing chicken hock joint samples with positive or negative M. synoviae infection. This research provides a foundation for the study of species-specific differences and molecular diagnosis of M. synoviae.

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“…The assembled genomes (18DW, 51SH, BS4S2, G3, and A4) and the genomes of MS-H [12] and WVU1853 [13] from GenBank were annotated using the Prokka, a genome annotation tool [14]. The average length of the assembled genomes was 795,375 bp.…”

Section: General Characteristics and Annotation Of The Assembled Ms Genomesmentioning

confidence: 99%

“…Each MS isolated from farms in Korea from 2017 to 2020, MS-H vaccine (Vaxsafe MS, Bioproperties, Australia) and WVU1853 (ATCC 25204) were also used in this study. Fasta files from accession number CP021129 and CP011096 were used to retrieve the genomes of MS-H [12] and WVU1853 [13], respectively (Table 5). MFB with and without 0.01% NAD supplement (Sigma-aldrich, Saint Louis, MO, USA) were used to incubate the MS isolates from Korea, strain WVU1853 and MS-H vaccine.…”

Section: Ms Strains Used In This Studymentioning

confidence: 99%

Genome Analysis of Nicotinamide Adenine Dinucleotide-Independent Mycoplasma synoviae Isolates From Korea

Song

Kim

et al. 2021

Pathogens

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Mycoplasma synoviae (MS) is an avian pathogen that causes respiratory disease, infectious synovitis, and eggshell apex abnormalities in chickens. Nicotinamide adenine dinucleotide (NAD)-independent MS was first reported in 1975. Despite the atypical traits of NAD-independent MS, its independence from NAD has not been studied. In this study, we isolated five NAD-independent strains from Korea and assembled their genomes using sequencing reads obtained from Illumina and Oxford Nanopore Technology platforms. The assembled genomes were compared with the genomes of MS-H vaccine strain and type strain WVU1853. We found that the coding sequences of nicotinate phosphoribosyltransferase and glycerol-3-phosphate acyltransferase, and a unique coding sequence were present only in the genomes of NAD-independent isolates.

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Complete Genome Sequence of Mycoplasma synoviae Strain WVU 1853 ^T

Cited by 7 publications

References 11 publications

Genome analysis of Mycoplasma synoviae strain MS-H, the most common M. synoviae strain with a worldwide distribution

Genome analysis of Mycoplasma synoviae strain MS-H, the most common M. synoviae strain with a worldwide distribution

Comparative Genomics of Mycoplasma synoviae and New Targets for Molecular Diagnostics

Genome Analysis of Nicotinamide Adenine Dinucleotide-Independent Mycoplasma synoviae Isolates From Korea

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Complete Genome Sequence of Mycoplasma synoviae Strain WVU 1853 T

Cited by 7 publications

References 11 publications

Genome analysis of Mycoplasma synoviae strain MS-H, the most common M. synoviae strain with a worldwide distribution

Genome analysis of Mycoplasma synoviae strain MS-H, the most common M. synoviae strain with a worldwide distribution

Comparative Genomics of Mycoplasma synoviae and New Targets for Molecular Diagnostics

Genome Analysis of Nicotinamide Adenine Dinucleotide-Independent Mycoplasma synoviae Isolates From Korea

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Complete Genome Sequence of Mycoplasma synoviae Strain WVU 1853 ^T