Comparative Genomics of Mycoplasma synoviae and New Targets for Molecular Diagnostics

Xu, Bin; Chen, Xi; Lu, Fengying; Sun, Yu; Sun, Huawei; Zhang, Jingfeng; Shen, Liya; Pan, Qunxing; Liu, Chuanmin; Zhang, Xiaofei

doi:10.3389/fvets.2021.640067

Cited by 6 publications

(4 citation statements)

References 49 publications

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“…The comparative genome analysis of the NAD-independent genomes revealed the unique CDSs present only in NAD-independent MS strains, viz., hypothetical CDS, nicotinate phosphoribosyltransferase CDS, and glycerol-3-phosphatase acyltransferase CDS. These CDSs were also found in the genomes of the MS strains HN01 [17] and FJ-01 from China. It is not known whether these two strains are NAD-independent.…”

Section: Discussionmentioning

confidence: 68%

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Genome Analysis of Nicotinamide Adenine Dinucleotide-Independent Mycoplasma synoviae Isolates From Korea

Song

Kim

et al. 2021

Pathogens

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Mycoplasma synoviae (MS) is an avian pathogen that causes respiratory disease, infectious synovitis, and eggshell apex abnormalities in chickens. Nicotinamide adenine dinucleotide (NAD)-independent MS was first reported in 1975. Despite the atypical traits of NAD-independent MS, its independence from NAD has not been studied. In this study, we isolated five NAD-independent strains from Korea and assembled their genomes using sequencing reads obtained from Illumina and Oxford Nanopore Technology platforms. The assembled genomes were compared with the genomes of MS-H vaccine strain and type strain WVU1853. We found that the coding sequences of nicotinate phosphoribosyltransferase and glycerol-3-phosphate acyltransferase, and a unique coding sequence were present only in the genomes of NAD-independent isolates.

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Section: Discussionmentioning

confidence: 68%

“…A 291-bp long hypothetical protein CDS, which could not be annotated by the Prokaryotic Genome Annotation Pipeline (PGAP), was present on the genomes of the Chinese MS isolate, HN01 [17] and FJ-01 with query coverage of 100% and pairwise identity of 99.7% on both genomes.…”

Section: Analysis Using Roary and Megablastmentioning

confidence: 99%

Genome Analysis of Nicotinamide Adenine Dinucleotide-Independent Mycoplasma synoviae Isolates From Korea

Song

Kim

et al. 2021

Pathogens

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“…Total DNA from collected laryngeal swabs was extracted using Hi-Swab DNA Kit (TIANGEN Biotech Co., Ltd) according to the manufacturer’s instructions. A standard plasmid containing the target sequence from the M. synoviae genome was constructed to analyze the data using the absolute quantitative method, following a previously described protocol with some modifications 56 . In brief, to obtain M. synoviae partial targeted gene sequences (Supplementary Fig.…”

Section: Methodsmentioning

confidence: 99%

Screening of immunogenic proteins and evaluation of vaccine candidates against Mycoplasma synoviae

Han

Chen

et al. 2023

npj Vaccines

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Mycoplasma synoviae (M. synoviae) is a serious avian pathogen that causes significant economic losses to chicken and turkey producers worldwide. The currently available live attenuated and inactivated vaccines provide limited protection. The objective of this study was to identify potential subunit vaccine candidates using immunoproteomics and reverse vaccinology analyses and to evaluate their preliminary protection. Twenty-four candidate antigens were identified, and five of them, namely RS01790 (a putative sugar ABC transporter lipoprotein), BMP (a substrate-binding protein of the BMP family ABC transporter), GrpE (a nucleotide exchange factor), RS00900 (a putative nuclease), and RS00275 (an uncharacterized protein), were selected to evaluate their immunogenicity and preliminary protection. The results showed that all five antigens had good immunogenicity, and they were localized on the M. synoviae cell membrane. The antigens induced specific humoral and cellular immune responses, and the vaccinated chickens exhibited significantly greater body weight gain and lower air sac lesion scores and tracheal mucosal thicknesses. Additionally, the vaccinated chickens had lower M. synoviae loads in throat swabs than non-vaccinated chickens. The protective effect of the RS01790, BMP, GrpE, and RS00900 vaccines was better than that of the RS00275 vaccine. In conclusion, our study demonstrates the potential of subunit vaccines as a new approach to developing M. synoviae vaccines, providing new ideas for controlling the spread of M. synoviae worldwide.

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“…Total DNA from collected laryngeal swabs was extracted using according to the manufacturer's instructions. The standard plasmid was constructed containing M. synoviae genome target to analyze the data using the absolute quantitative method as described previously with some modifications 56 . In brief, standard curves were prepared using purified M. synoviae plasmid.…”

Section: Quantification Of Cytokine Genes Expression and M Synoviae Dnamentioning

confidence: 99%

Novel antigenic proteins of Mycoplasma synoviae as potential vaccine candidates

Shi

Han

et al. 2023

Preprint

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Mycoplasma synoviae (M. synoviae) is a serious avian pathogen causing severe economic losses to chicken and turkey producers worldwide. The currently available live attenuated vaccine and inactivated vaccine on the market elicit only limited protection. The aim of this study was to identify antigenic proteins of M. synoviae as potential subunit vaccine candidates. In immunoproteomics and reverse vaccinology analyses, a total 24 candidate antigens were picked out. Five candidate antigens were selected to evaluate immunogenicity and preliminary protection in a chicken model, including RS01790 (lipoprotein, putative sugar ABC transporter), BMP (BMP family ABC transporter substrate-binding protein), GrpE (nucleotide exchange factor), RS00900 (putative nuclease) and RS00275 (Uncharacterized protein). The results showed that the five antigens had good immunogenicity. They could be localized on the M. synoviae cell membrane and adhere to DF-1 cells using indirect immunofluorescence assay. They induced specific humoral and cellular immune responses. Vaccine efficacy was evaluated by observing weight gain, pathogen load, pathological changes. As a result, the vaccinated chickens revealed significantly higher body weight gain, with lower air sac lesion scores and tracheal mucosal thicknesses. The vaccinated chickens also showed lower M. synoviae loads in throat swabs than nonvaccinated chickens. The protective effect of BMP, GrpE and RS00900 vaccines is better than that of RS01790 and RS00275. In conclusion, our study demonstrates the potential of using subunit vaccine, providing new ideas for the development of M. synoviae vaccines. The vaccine candidates may contribute to the future development of therapeutic strategies to control the spread of M. synoviae worldwide.

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Comparative Genomics of Mycoplasma synoviae and New Targets for Molecular Diagnostics

Cited by 6 publications

References 49 publications

Genome Analysis of Nicotinamide Adenine Dinucleotide-Independent Mycoplasma synoviae Isolates From Korea

Genome Analysis of Nicotinamide Adenine Dinucleotide-Independent Mycoplasma synoviae Isolates From Korea

Screening of immunogenic proteins and evaluation of vaccine candidates against Mycoplasma synoviae

Novel antigenic proteins of Mycoplasma synoviae as potential vaccine candidates

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