Complete Genome Sequence of Multiple-Antibiotic-Resistant Streptococcus parauberis Strain SPOF3K, Isolated from Diseased Olive Flounder (Paralichthys olivaceus)

Lee, Yoonhang; Nguyen, Thanh Luan; Kim, Ahran; Kim, Nameun; Roh, Heyong Jin; Han, Hyun‐Ja; Jung, Sung‐Hee; Cho, Young Min; Kang, Ho Young; Kim, Do-Hyung

doi:10.1128/genomea.00248-18

Cited by 11 publications

(11 citation statements)

References 24 publications

(23 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A plasmid of S . parauberis SPOF3K contains 13 genes [ 9 ]. Of these genes, inlJ (SPSF3K_02212) [ 25 ] and tetS (SPSF3K_02213) [ 26 ] have been implicated in the pathogen’s virulence and antibiotic resistance, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…The SPOF3K, virulent strain of Streptococcus parauberis used in this study was isolated from the kidney of a diseased olive flounder ( Paralichthys olivaceus ) in Geojedo, Korea in 2013. The genome of strain SPOF3K was sequenced, annotated, and demonstrated in our previous study [ 9 ]. Healthy one-year-old olive flounders weighing approximately 200 g were purchased from a commercial fish farm in Busan, South Korea.…”

Section: Methodsmentioning

confidence: 99%

“…Raw Illumina sequence reads were evaluated and trimmed using FastQC [ 18 ] to remove low-quality reads. The remaining reads were mapped onto a reference SPOF3K genome [ 9 ] (accession numbers: CP025420.1, CP025421.1) using Bowtie2 [ 19 ]. DESeq2 [ 20 ] was used for data normalization and differential gene expression (DGE) analysis by uploading read counts for each gene into iDEP.92 [ 21 ].…”

Section: Methodsmentioning

confidence: 99%

“…Recently, several genomic studies have been done on S . parauberis derived from a variety of fish species including the olive flounder and the data is being made publicly available [ 8 , 9 ]. However, there is a limited amount of experimental data on functional characterization of genetic components.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Transcriptome analysis unveils survival strategies of Streptococcus parauberis against fish serum

et al. 2021

Self Cite

View full text Add to dashboard Cite

Streptococcus parauberis is an important bacterial fish pathogen that causes streptococcosis in a variety of fish species including the olive flounder. Despite its importance in the aquaculture industry, little is known about the survival strategy of S. parauberis in the host. Therefore, the objective of this study was to produce genome-wide transcriptome data and identify key factors for the survival of S. parauberis SPOF3K in its host. To this end, S. parauberis SPOF3K was incubated in olive flounder serum and nutrient-enriched media as a control. Although S. parauberis SPOF3K proliferated in both culture conditions, the transcriptomic patterns of the two groups were very different. Interestingly, the expression levels of genes responsible for the replication of an S. parauberis plasmid in the presence of olive flounder serum were higher than those in the absence of olive flounder serum, indicating that this plasmid may play an important role in the survival and proliferation of S. parauberis in the host. Several ATP-binding cassette transporters known to transport organic substrates (e.g., biotin and osmoprotectants) that are vital for bacterial survival in the host were significantly up-regulated in S. parauberis cultured in serum. In addition, groEL, dnaK operon, and members of the clp protease family, which are known to play important roles in response to various stressors, were up-regulated in S. parauberis incubated in serum, thus limiting damage and facilitating cellular recovery. Moreover, important virulence factors including the hyaluronic acid capsule (has operon), sortase A (srtA), C5a peptidase (scp), and peptidoglycan O-acetyltransferase (oatA) were significantly upregulated in S. paraubers in serum. These results indicate that S. paraubers can resist and evade the humoral immune responses of fish. The transcriptomic data obtained in this study provide a better understanding of the mode of action of S. parauberis in fish.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Transcriptome analysis unveils survival strategies of Streptococcus parauberis against fish serum

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…In contrast, strains of this species are susceptible to cefotaxime, ofloxacin and vancomycin [65]. However, resistance to β-lactam antibiotics has been demonstrated in studies with strains isolated from fish [66]. Table 4 shows details of the clinical cases caused by confirmed S. uberis and S. parauberis strains.…”

Section: Streptococcus Uberismentioning

confidence: 99%

Pathogenicity and drug resistance of animal streptococci responsible for human infections

Glajzner

Szewczyk

Szemraj

2021

Journal of Medical Microbiology

View full text Add to dashboard Cite

Bacteria of the genus Streptococcus , earlier considered typically animal, currently have also been causing infections in humans. It is necessary to make clinicians aware of the emergence of new species that may cause the development of human diseases. There is an increasing frequency of isolation of streptococci such as S. suis , S. dysgalactiae , S. iniae and S. equi from people. Isolation of Streptococcus bovis/Streptococcus equinus complex bacteria has also been reported. The streptococcal species described in this review are gaining new properties and virulence factors by which they can thrive in new environments. It shows the potential of these bacteria to changes in the genome and the settlement of new hosts. Information is presented on clinical cases that concern streptococcus species belonging to the groups Bovis, Pyogenic and Suis. We also present the antibiotic resistance profiles of these bacteria. The emerging resistance to β-lactams has been reported. In this review, the classification, clinical characteristics and antibiotic resistance of groups and species of streptococci considered as animal pathogens are summarized.

show abstract

Streptococcus

Samanta¹,

Bandyopadhyay²

2020

Antimicrobial Resistance in Agriculture

View full text Add to dashboard Cite

Complete Genome Sequence of Multiple-Antibiotic-Resistant Streptococcus parauberis Strain SPOF3K, Isolated from Diseased Olive Flounder (Paralichthys olivaceus)

Cited by 11 publications

References 24 publications

Transcriptome analysis unveils survival strategies of Streptococcus parauberis against fish serum

Transcriptome analysis unveils survival strategies of Streptococcus parauberis against fish serum

Pathogenicity and drug resistance of animal streptococci responsible for human infections

Streptococcus

Contact Info

Product

Resources

About