Complete genome sequence of Lactobacillus rhamnosus Pen, a probiotic component of a medicine used in prevention of antibiotic-associated diarrhoea in children

Abstract

BackgroundLactobacillus rhamnosus Pen is a human endogenous strain with well-documented health promoting properties that is used for production of probiotics. It has a long safety history of application, and its effectiveness in the prevention of antibiotic-associated diarrhoea has also been confirmed in clinical trials.ResultsHere we present the complete genome sequence of L. rhamnosus Pen, which consists of a circular 2,884,4966-bp chromosome with a GC content of 46.8%. Within 2907 open reading frames (ORFs), genes involved with probiotic properties were identified. A CRISPR locus, consisting of a 1092-nt region with 16 spacers, was also detected. Finally, an intact prophage of ~ 40.7 kb, 57 ORFs, GC content 44.8% was identified.ConclusionsGenomic analysis confirmed the probiotic properties of L. rhamnosus Pen and may indicate new biotechnological applications of this industrially important strain.Electronic supplementary materialThe online version of this article (10.1186/s13099-018-0235-z) contains supplementary material, which is available to authorized users.

Complete Genome Sequencing of Lactobacillus plantarum ZLP001, a Potential Probiotic That Enhances Intestinal Epithelial Barrier Function and Defense Against Pathogens in Pigs

“…Lactobacillus plantarum ZLP001 genome contained 22 CRISPR loci (CRISPR 1 to CRISPR 22), including 9 CRISPR loci in the chromosome and 13 CRISPR loci in the plasmids (Table S5 ). The detected CRISPR/CRISPR-associated (Cas) system in plasmid C was type II-A (four cas genes; cas 1, cas 2, cas 9, and csn 2), consistent with the previously described CRISPR loci characteristic of L. rhamnosus Pen (Jarocki et al, 2018 ).…”

Genomic and Proteomic Characterization of Bacteriophage BH1 Spontaneously Released from Probiotic Lactobacillus rhamnosus Pen

“…The G + C content of BH1 genome was 44.8%; very close to previously described bacteriophages for the Lactobacillus casei group: Lc-Nu (44.3%), φAT3 (44.6%), A2 (44.9%), Lca1 (44.8%), and Lrm1 (45.5%). The G + C content also corresponded to the overall GC-content determined for chromosomal DNA of bacterial host of BH1-L. rhamnosus Pen (46.8%), and for other previously reported L. rhamnosus strains (average value of 46.7%) [22][23][24].…”

Genome analysis of a wild rumen bacterium Enterobacter aerogenes LU2 - a novel bio-based succinic acid producer

“…However, it can increase the possibility of phage lysis during industrial processes in bioreactors. Moreover, the presence of a prophage in the genome may increase resistance to environmental stresses, biofilm formation and supporting of horizontal gene transfer that contributes to an increase in biodiversity 44,45 . Taking into account that the LU2 strain was isolated from cow rumen, these features arising from the presence of a prophage presence may provide benefits to the bacterial host.…”