Complete Genome Sequencing of Lactobacillus plantarum ZLP001, a Potential Probiotic That Enhances Intestinal Epithelial Barrier Function and Defense Against Pathogens in Pigs

Zhang, Wei; Ji, Hai‐Feng; Zhang, Dongyan; Liu, Hui; Wang, Sixin; Wang, Jing; Wang, Yamin

doi:10.3389/fphys.2018.01689

Cited by 42 publications

(32 citation statements)

References 45 publications

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“…37 Genes encoding the complete GSH system, including GSH peroxidase and GSH reductase, have been identified in L. plantarum ZLP001. 38 L. plantarum ZLP001 also harbors genes for a complete thioredoxin system, including thioredoxin, thioredoxin reductase, and thiol peroxidase. However, L. plantarum ZLP001 does not harbor SOD genes, implying that different LAB species encode different redox-related genes and have different redox systems.…”

Section: Genes Associated With Redox In Probiotic Labmentioning

confidence: 99%

Oxidative stress tolerance and antioxidant capacity of lactic acid bacteria as probiotic: a systematic review

2020

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Lactic acid bacteria (LAB) are the most frequently used probiotics in fermented foods and beverages and as food supplements for humans or animals, owing to their multiple beneficial features, which appear to be partially associated with their antioxidant properties. LAB can help improve food quality and flavor and prevent numerous disorders caused by oxidation in the host. In this review, we discuss the oxidative stress tolerance, the antioxidant capacity related herewith, and the underlying mechanisms and signaling pathways in probiotic LAB. In addition, we discuss appropriate methods used to evaluate the antioxidant capacity of probiotic LAB. The aim of the present review is to provide an overview of the current state of the research associated with the oxidative stress tolerance and antioxidant capacity of LAB.

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Section: Genes Associated With Redox In Probiotic Labmentioning

confidence: 99%

Oxidative stress tolerance and antioxidant capacity of lactic acid bacteria as probiotic: a systematic review

2020

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“…Orthologous clustering is frequently used for the identification of specific genes in specific bacterial species or strains (17,18). We found 16 to 175 strain-specific genes in L. citreum strains, which accounted for 1% to 9% of total CDSs in each strain.…”

Section: Discussionmentioning

confidence: 87%

Pseudofructophilic Leuconostoc citreum Strain F192-5, Isolated from Satsuma Mandarin Peel

Maeno

Tanizawa

Kajikawa

et al. 2019

Appl Environ Microbiol

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Fructophilic lactic acid bacteria (FLAB), composed of Fructobacillus spp., Lactobacillus kunkeei, and Lactobacillus apinorum, are unique in that they prefer d-fructose over d-glucose as a carbon source. Strain F192-5, isolated from the peel of a satsuma mandarin and identified as Leuconostoc citreum, grows well on d-fructose but poorly on d-glucose and produces mainly lactate and acetate, with trace amounts of ethanol, from the metabolism of d-glucose. These characteristics are identical to those of obligate FLAB. However, strain F192-5 ferments a greater variety of carbohydrates than known FLAB. Comparative analyses of the genomes of strain F192-5 and reference strains of L. citreum revealed no signs of specific gene reductions, especially genes involved in carbohydrate transport and metabolism, in the genome of F192-5. The bifunctional alcohol/acetaldehyde dehydrogenase gene (adhE) is conserved in strain F192-5 but is not transcribed. This is most likely due to a deletion in the promoter region upstream of the adhE gene. Strain F192-5 did, however, ferment d-glucose when transformed with a plasmid containing the allochthonous adhE gene. L. citreum F192-5 is an example of a pseudo-FLAB strain with a deficiency in d-glucose metabolism. This unique phenotypic characteristic appears to be strain specific within the species L. citreum. This might be one of the strategies lactic acid bacteria use to adapt to diverse environmental conditions. IMPORTANCE Obligate fructophilic lactic acid bacteria (FLAB) lack the metabolic pathways used in the metabolism of most carbohydrates and differ from other lactic acid bacteria in that they prefer to ferment d-fructose instead of d-glucose. These characteristics are well conserved at the genus or species level. Leuconostoc citreum F192-5 shows similar growth characteristics. However, the strain is metabolically and genomically different from obligate FLAB. This is an example of a strain that evolved a pseudo-FLAB phenotype to adapt to a fructose-rich environment.

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“…Bifidoprophages are present in a small number in multiple bifidobacterial species, indicating their relatedness to phages infecting other Actinobacteria, Firmicutes,as well as gram-negative bacteria [21,22]. In addition, integrases are useful indicators of prophage diversity in bacterial genomes [23]. Prophage region 1 (804,476 -847,197 bp) contained 58 CDSs, with a complete prophage integrase gene (Additional file 3).…”

Section: Phastmentioning

confidence: 99%

“…These genes are induced to respond upon exposure of bifidobacterial cultures to stressful condition, and overexpression of sHsps is known to increase tolerance to heat and osmotic stress in B. breve and B. longum NCC2705 [27]. CACC 514 encodes 2 genes for sodiumproton antiporter (Na +/H +), providing evidence of tolerance to low pH in the GI environment [23].…”

Section: Stress-related Proteinsmentioning

confidence: 99%

Genomic insights and probiotic characteristics of Bifidobacterium gallinarum CACC 514 isolated from canine

Jung¹,

Kim²,

Kim³

et al. 2019

Preprint

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Background: The genus Bifidobacterium includes common healthy gut microbes in mammals and is widely acknowledged as a probiotics with health-promoting properties. Results: Bifidobacterium gallinarum CACC 514 was isolated from canine feces and its potential probiotic properties were characterized by comparative and functional genome analyses.The complete genome of strain CACC 514 was found to be 2.4 Mb, with a G + C content of 63.9 mol%. The strain possessed factors beneficial to mammalian health based on the presence of genes related to mucosal surface adhesion proteins, stress-related genes, and extracellular polysaccharide genes. A comparative genome analysis with other Bifidobacterium species revealed the unique characteristics of this species. These functional genomics have been confirmed for its superiority by probiotic properties, acid and bile tolerance, and adhesion to mucus. Conclusions: The genome analysis and in vitro probiotics characteristics revealed its superiority in the intestine. These results add to our comprehensive understanding of B. gallinarum and suggest that this strain has potential application in mammalian probiotics. Keywords: Bifidobacterium gallinarum, gut microbe, probiotics, genome analysis, canine, feces.

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Complete Genome Sequencing of Lactobacillus plantarum ZLP001, a Potential Probiotic That Enhances Intestinal Epithelial Barrier Function and Defense Against Pathogens in Pigs

Cited by 42 publications

References 45 publications

Oxidative stress tolerance and antioxidant capacity of lactic acid bacteria as probiotic: a systematic review

Oxidative stress tolerance and antioxidant capacity of lactic acid bacteria as probiotic: a systematic review

Pseudofructophilic Leuconostoc citreum Strain F192-5, Isolated from Satsuma Mandarin Peel

Genomic insights and probiotic characteristics of Bifidobacterium gallinarum CACC 514 isolated from canine

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