Complete Genome Sequence of Emerging Porcine Circovirus Types 2a and 2b from India

Bhattacharjee, Uttaran; Ahuja, Anuj; Sharma, Indu; Karam, Amarjit; Chakraborty, A. K.; Ghatak, Sandeep; Puro, K.; Das, Samir; Shakuntala, I.; Giri, Sarbani; Pegu, Raj Kumar; Laha, R.; Pattanayak, A.; Ngachan, S. V.

doi:10.1128/genomea.00087-15

Cited by 5 publications

(6 citation statements)

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“…It has been shown that the intergenotypic recombination between the PCV2 might lead to a variant genotype with a different pathogenic potential to pig population in India (Anoopraj et al., ). The complete genome sequence analysis of PCV2 strains from NER has been reported (Bhattacharjee et al., ). Our study is in continuation of our ongoing seroprevalence of PCV2 in NER of India.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, lack of diagnostic along with proper control measures adds in the hindrance to the development of pig husbandry. In the last 10 years, porcine circovirus 2 (PCV2) infections emerged as a new threat to the pig population of NER (Bhattacharjee et al., ; Mukherjee et al., ). The postweaning multisystemic wasting syndrome (PMWS) caused by novel strains of PCV2 has emerged as a major cause of piglet mortality.…”

Section: Introductionmentioning

confidence: 99%

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The emergence of porcine circovirus 2 infections in the Northeastern part of India: A retrospective study from 2011 to 2017

Barman

Nath

Kumar

et al. 2018

Transbound Emerg Dis

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Porcine circovirus (PCV) infection has emerged as an alarming threat to the pig population of India, especially in the Northeastern region (NER) over the last 10 years. The present study is a comprehensive report of the seroepidemiology of PCV2 and its incidences in the pig population from organized and unorganized farms of the entire NER of India from 2011 to 2017. A total of 5697 serum samples were screened by ELISA and the mean positivity of PCV2 antibodies in suspected sera was 31.27%. A total of 22 confirmed cases of PCV2 infection were recorded during the years 2014-2017. Seroprevalence of PCV2 infection in sows causing reproductive disorders in NER suggested its higher incidence in organized farms (65.7%) as compared to unorganized farms (17.6%). A detailed pathological and histopathological examination of the tissue samples collected from the affected animals indicated the presence of PCV2. Molecular characterization and phylogenetic analysis of four PCV2 isolates depicted the circulation of PCV2d genotype in the states of Meghalaya and Assam.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The emergence of porcine circovirus 2 infections in the Northeastern part of India: A retrospective study from 2011 to 2017

Barman

Nath

Kumar

et al. 2018

Transbound Emerg Dis

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“…Signature motifs and phylogenetic analysis of 13 PCV2 ORF2 full‐length sequences in this study revealed the presence of PCV2b, PCV2b‐IM1, PCV2d, and PCV2d‐2 genotypes circulating in the field. The prevalence of PCV2 infections in India is raising gradually with the involvement of PCV2a, PCV2b, PCV2d, and recombinant genotypes in the field (Anoopraj et al., 2015; Bhattacharjee et al 2015; Karuppannan et al., 2016, Pegu et al., 2017; Mukherjee et al., 2018). Thangthamniyom et al.…”

Section: Discussionmentioning

confidence: 99%

Emergence of novel Porcine circovirus 2 genotypes in Southern India

Parthiban¹,

Karuppannan²,

et al. 2021

Transbounding Emerging Dis

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A total of 200 samples from Porcine circovirus 2 suspected (n = 112) and healthy (n = 88) swine populations collected from different districts of Tamil Nadu, south India were used in this study. The samples comprising of serum (n = 124), swabs from natural orifices (n = 52), and postmortem tissues (n = 24). All the samples were processed and subjected to the screening and detection of the PCV2 genome by a specific PCR assay.PCV2 genomes from positive samples were further subjected to genotyping with specifically designed primers for the full-length amplification of the ORF2 gene which codes for capsid protein (Cp) and serves as an epidemiological marker. Randomly, 13 amplified ORF2 genes were sequenced and the aligned sequences were subjected to signature motif analysis and phylogeny in MEGA X. The molecular prevalence of PCV2 infection in Tamil Nadu is 10.5% (n = 21). Signature motif and phylogenetic studies of 13 samples revealed 38.5% (n = 5) presence of each PCV2b intermediate 1(IM1) and PCV2b genotypes, followed by 15.4% (n = 2) PCV2d-2 and 7.7% (n = 1) PCV2d genotypes. The PCV2b-IM1 genotype has a 99.43% sequence homology with Vietnam isolate (JX506730). PCV2b genotypes showed 99.72% sequence identity with Chinese isolate (KX068219). PCV2d-2 genotypes reported in this study have 100% sequence identity with Taiwan isolate (MF169721). PCV2d genotype showed 97.87% sequence identity with Thailand isolate (MF314293). Amino acid analysis of all the 13 full-length ORF2 gene sequences revealed specific mutations in the immune reactive domains of A, B, C, and D. Capsid protein of three PCV2b and five PCV2b IM1 isolates had extra amino acid residue lysine (K) at 234 position of ORF2 similar to PCV2d. For the first time in South India, PCV2b IM1 and PCV2d-2 genotypes are reported. This study evidences the genetic shifts of PCV2 isolates in India and it is analogous to that of global genotypic shift.

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“…Emergence of new recombinant genotype PCV1/2a, having ORF1 of PCV1 and ORF2 of PCV2, has been reported in Canada with very low prevalence rate in 2008 (Gagnon et al 2010). In Northeast India, emergence of PCV2a and PCV2b has been reported (Bhattacharjee et al 2015). A novel genotype PCV2f was reported in three out of 23 PCV2 strains identified from the archived pig tissue samples collected between 1996 and 1999 in China (Bao et al 2018).…”

Section: Virusmentioning

confidence: 99%

Porcine Circovirus

Saikumar

Das

2019

Recent Advances in Animal Virology

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Porcine circovirus (PCV) infections associated with post-weaning multisystemic wasting syndrome (PMWS) are characterized by weight loss, respiratory distress, jaundice, etc. Although PCV2 infection is ubiquitous, the prevalence of clinical disease is lower and the most common form is PCV2 subclinical infection. Recently, a novel porcine circovirus (PCV3) has been identified in pigs in the USA that is associated with porcine dermatitis nephropathy syndrome, acute myocarditis and multisystemic inflammation, etc. Genetic heterogeneity of PCV2 has been studied in Indian pig population. Different genotypes like PCV2a-2D, PCV2b-1C, PCV2d and recombinant strain between PCV2a-2C and PCV2b-1C are reported from different studies. PCV2 has been discovered in human faeces, human vaccines and beef. But its pathogenicity in humans is not clear. PCV detection is based on common golden standard techniques including nucleic acid and antigen detection in the tissues, in situ hybridization (ISH) and immunohistochemistry (IHC) using monoclonal or polyclonal antibody against PCV2, respectively. The commercial vaccines available are effective in reducing the severity of clinical diseases and improving production parameters. Recently, antiviral compounds have also shown promising results against PCV2. This chapter summarizes aetiology, epidemiology, transmission, immunopathobiology, diagnosis, prevention and control of porcine circovirus.

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Complete Genome Sequence of Emerging Porcine Circovirus Types 2a and 2b from India

Abstract: We report here the first characterized complete genome sequence of porcine circovirus types 2a and 2b from northeastern states of India. These isolates may serve as a potential reference for the Indian strains of porcine circovirus types 2a and 2b.

Cited by 5 publications

References 7 publications

The emergence of porcine circovirus 2 infections in the Northeastern part of India: A retrospective study from 2011 to 2017

The emergence of porcine circovirus 2 infections in the Northeastern part of India: A retrospective study from 2011 to 2017

Emergence of novel Porcine circovirus 2 genotypes in Southern India

Porcine Circovirus

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