Complete Genome Sequence of Ehrlichia mineirensis, a Novel Organism Closely Related to Ehrlichia canis with a New Host Association

Cabezas-Cruz, Alejandro; Zweygarth, E.; Broniszweska, Marzena; Passos, Lygia Maria Friche; Ribeiro, Múcio Flávio Barbosa; Manrique, Marina; Tobes, Raquel; Fuente, José de la

doi:10.1128/genomea.01450-14

Cited by 11 publications

(5 citation statements)

References 16 publications

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“…The 16S rRNA phylogenetic tree shows that Ehrlichia is a diverse genus (7), and despite recent improvements in bacterial genome sequencing technologies, only a few genomes are available for Ehrlichia species, including those of E. chaffeensis , E. ruminantium , E. canis , E. muris , and E. minasensis UFMG-EV T (15). Here, we report the genome sequence of E. minasensis strain Cuiabá, isolated from whole blood of an 8-month-old naturally infected male Holstein calf.…”

Section: Announcementmentioning

confidence: 99%

Complete Genome Sequence of an Ehrlichia minasensis Strain Isolated from Cattle

Aguiar

Araújo

Nakazato

et al. 2019

Microbiol Resour Announc

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Section: Announcementmentioning

confidence: 99%

Complete Genome Sequence of an Ehrlichia minasensis Strain Isolated from Cattle

Aguiar

Araújo

Nakazato

et al. 2019

Microbiol Resour Announc

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“…UFMG-EV T using DNA extracted from Ehrlichia purified from IDE8 cells (Cabezas-Cruz et al, 2015). The genome of Ehrlichia sp.…”

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confidence: 99%

“…UFMG-EV T resulted in 944 genes, including protein-coding sequences (CDSs), RNA genes and pseudogenes. Of them, 322 genes encode proteins with enzymic activity, 51 encode membrane proteins, 55 are involved in DNA repair and 144 are connected with oxidoreduction processes (Cabezas-Cruz et al, 2015). Taking in account the scarcity of genomic data from the genus Ehrlichia (Fig.…”

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confidence: 99%

Ehrlichia minasensis sp. nov., isolated from the tick Rhipicephalus microplus

Cabezas-Cruz

Zweygarth

Vancová

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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Recently, we obtained a rickettsial isolate (Ehrlichia sp. UFMG-EV T ) from the haemolymph of engorged Rhipicephalus microplus tick females. On the basis of maximum-likelihood phylogenetic analysis using 16S rRNA gene, groEL, dsb, gltA and trp36 sequences we showed that Ehrlichia sp. UFMG-EV T belongs to the a-Proteobacteria, family Anaplasmataceae, genusEhrlichia. Ehrlichia sp. UFMG-EV T is a sister taxon of Ehrlichia canis with 16S rRNA gene, groEL, dsb, gltA and trp36 sequence similarities of 98.3 %, 97.2 %, 94.7 %, 94.3 % and 49.1 %, respectively. Ehrlichia sp. UFMG-EV T has been maintained in the laboratory by continuous passage in the IDE8 tick cell line where the ultrastructure was characterized using electron microscopy and was found to resemble that of E. canis, Ehrlichia muris and Ehrlichia chaffeensis, but not Ehrlichia ruminantium and Ehrlichia ewingii. We propose the name Ehrlichia minasensis sp. nov. for this bacterium to acknowledge the place from where it was initially isolated, Minas Gerais, Brazil. The type strain is strain Ehrlichia sp.

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“…Ehrlichia minasensis was first described in cattle from Canada [36], and later in R. microplus ticks and cattle from Brazil [7,10,36,37]. Additionally, E. minasensis has been the subject of studies on morphological and genetic characterization [38] and their distribution has been shown in recent reports in Corsica, France [14], Pakistan [15], Ethiopia [39], South Africa [13], Israel [40] and China [16].…”

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confidence: 99%

First Evidence of Ehrlichia minasensis Infection in Horses from Brazil

et al. 2021

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The genus Ehrlichia includes tick-borne bacterial pathogens affecting humans, domestic and wild mammals. Ehrlichia minasensis has been identified in different animal species and geographical locations, suggesting that this is a widely distributed and generalist Ehrlichia. In the present study, we evaluated Ehrlichial infection in 148 Equidae presented to the Medical Clinic Department of a Veterinary Hospital from a midwestern region of Brazil. Blood samples and ticks collected from the animals were tested by Polymerase Chain Reaction (PCR) for the presence of Ehrlichia spp. A multigenic approach including Anaplasmataceae-specific (i.e., 16S rRNA, groEL, gltA) and Ehrlichia-specific (i.e., dsb and trp36) genes was used for accurate bacteria identification. Sera samples were also collected and evaluated for the detection of anti-Ehrlichia antibodies by indirect fluorescent antibody test (IFA). Possible associations between molecular and serological diagnostics and clinical and hematological manifestations were tested using chi-squared or Fisher’s exact tests. Sequence analysis of the dsb fragment revealed that three horses (2.03%) were exposed to E. minasensis. Sixty-one (41.2%) Equidae (58 equines and three mules), were seropositive for Ehrlichia spp., with antibody titers ranging between 40 and 2560. Seropositivity to ehrlichial antigens was statistically associated with tick infestation, rural origin, hypoalbuminemia and hyperproteinemia (p ≤ 0.05). The present study reports the first evidence of natural infection by E. minasensis in horses from Brazil.

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Complete Genome Sequence of Ehrlichia mineirensis, a Novel Organism Closely Related to Ehrlichia canis with a New Host Association

Cited by 11 publications

References 16 publications

Complete Genome Sequence of an Ehrlichia minasensis Strain Isolated from Cattle

Complete Genome Sequence of an Ehrlichia minasensis Strain Isolated from Cattle

Ehrlichia minasensis sp. nov., isolated from the tick Rhipicephalus microplus

First Evidence of Ehrlichia minasensis Infection in Horses from Brazil

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