Ehrlichia minasensis sp. nov., isolated from the tick Rhipicephalus microplus

Cabezas-Cruz, Alejandro; Zweygarth, E.; Vancová, Marie; Broniszewska, Marzena; Grubhoffer, Libor; Passos, Lygia Maria Friche; Ribeiro, Múcio Flávio Barbosa; Alberdi, Pilar; Fuente, José de la

doi:10.1099/ijsem.0.000895

Cited by 87 publications

(68 citation statements)

References 21 publications

(15 reference statements)

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“…Ehrlichia genotypes closely related to E. canis have already been detected in wild carnivores , wild birds and domestic cats (André et al, 2015) in Brazil, and rodents in Korea (Kim et al, 2006). In addition, Ehrlichia minasensis, a species closely related to Ehrlichia canis that has been identified infecting Rhipicephalus microplus ticks and cattle in Brazil (Carvalho et al, 2016;Cabezas-Cruz, et al;2016), also showed phylogenetic relatedness with the sequences obtained in the present study, but lower when compared to Ehrlichia canis. Following the same pattern observed with Anaplasma genotypes detected in the present study, the positive samples at cPCR and qPCR assays for Ehrlichia spp.…”

Section: Discussionsupporting

confidence: 61%

Assessment of a quantitative 5′ nuclease real-time polymerase chain reaction using groEL gene for Ehrlichia and Anaplasma species in rodents in Brazil

Benevenute

Dumler

Ogrzewalska

et al. 2017

Ticks and Tick-borne Diseases

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New genotypes of Anaplasmataceae agents have been detected in wild carnivores, birds and deer in Brazil. The present work aimed to investigate the presence of Ehrlichia and Anaplasma species in rodents sampled in Brazil. Additionally, a newly designed quantitative 5' nuclease real-time multiplex PCR for Ehrlichia and Anaplasma spp. detection based on groEL gene amplification was designed, showing high specificity and sensitivity (10 groEL fragment copy/μL). Between 2000 and 2011, different rodent species [n=60] were trapped in 5 Brazilian biomes. Among 458 rodent spleen samples, 0.4% (2/458) and 2.4% (11/458) were positive for Ehrlichia and Anaplasma spp., respectively. Of 458 samples, 2.0% (9/458) and 1.1% (5/458) were positive for Anaplasma sp. and Ehrlichia sp., respectively, using conventional 16S rRNA PCR assays. Maximum Likelihood phylogenetic analyse based on a small region of 16S rRNA genes positioned the Anaplasma genotypes in rodents near Anaplasma phagocytophilum or Anaplasma marginale and Anaplasma odocoilei isolates. Ehrlichia genotypes were closely related to E. canis. There was a low occurrence of Anaplasma and Ehrlichia in wild and synanthropic rodents in Brazil, suggesting the circulation of new genotypes of these agents in rodents in the studied areas.

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Section: Discussionsupporting

confidence: 61%

Assessment of a quantitative 5′ nuclease real-time polymerase chain reaction using groEL gene for Ehrlichia and Anaplasma species in rodents in Brazil

Benevenute

Dumler

Ogrzewalska

et al. 2017

Ticks and Tick-borne Diseases

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“…canis TWN (98.3% and 97.2% respectively) (Cabezas‐Cruz et al . ). Phylogenetic analyses proved that our Corsican strain was most closely related with E .…”

Section: Resultsmentioning

confidence: 97%

“…microplus female tick (Cabezas‐Cruz et al . ). Ehrlichia minasensis was established as a new species in 2016.…”

Section: Introductionmentioning

confidence: 97%

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First detection of Ehrlichia minasensis in Hyalomma marginatum ticks collected from cattle in Corsica, France

Cicculli

Masse

Capai

et al. 2019

Veterinary Medicine & Sci

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Ehrlichiosis are severe, feverish tick‐borne illnesses caused by specific species within the genus Ehrlichia (Anaplasmataceae family). Recent data suggest that ruminants in Corsica area reservoir for several Anaplasmataceae species. The purpose of our study was to determine whether Ehrlichia species could be detected in ticks collected in Corsican ruminants by using molecular methods. Ticks were collected in northern Corsica: (i) in May 2016 from sheep bred in one farm located in a 5000‐inhabitants village and (ii) from cattle in June and July 2016 in a slaughterhouse. There sheep and cattle whole skin was inspected and ticks were collected manually. A total of 647 ticks was collected in northern Corsica during this study: 556 (86%) belonged to the Rhipicephalus bursa species and 91 (14%) to Hyalomma marginatum . The 91 H . marginatum ticks were organized in 27 pools, of which one (3.7%) was found positive for the presence of E . minasensis ; this pool consisted of six ticks collected from a cow bred and raised northwestern Corsica. Ehrlichial DNA was not detected in R . bursa ticks. The 16S rRNA and groEL gene sequences of Ehrlichia detected in the H . marginatum pool showed 100% (303/303 bp) and 99.8% (555/556) of nucleotide identity with E . minasensis, respectively. Phylogenetic analyses demonstrated the highest closeness with E . minasensis UFMG‐EV genotype than to any other E . canis strains. To our knowledge, this is the first report of E . minasensis outside of Brazil, Ethiopia and Canada. This identification of E . minasensis in H . marginatum merits to be further investigated and pleads for translational studies addressing the potential impact of vector‐borne diseases of human and veterinary impact through large‐scale research and surveillance programmes in Corsica.

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“…Pritt et al (2011) identified a third species of Ehrlichia muris in patients who had fever, malaise, headache, and lymphopenia in Wisconsin and Minnesota, the United States, and it has also been found in Europe and Asia (Rar et al, 2010; Tateno et al, 2015). Other species that infect animals include A. marginale , Anaplasma centrale , A. ovis , E. canis , and Ehrlichia minasensis (Wen et al, 2003; Kocan et al, 2015; Cabezas-Cruz et al, 2016). Several species have been described in China, such as A. ovis , Anaplasma bovis , and A. platys in Gansu province (Li et al, 2016), A. centrale and E. canis in Fujian province (Ge et al, 2016; Yu et al, 2016), and E. chaffeensis in Guangxi province (Yang et al, 2015b).…”

Section: Introductionmentioning

confidence: 99%

Molecular Detection and Characterization of Zoonotic and Veterinary Pathogens in Ticks from Northeastern China

et al. 2016

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Tick-borne diseases are considered as emerging infectious diseases in humans and animals in China. In this study, Ixodes persulcatus (n = 1699), Haemaphysalis concinna (n = 412), Haemaphysalis longicornis (n = 390), Dermacentor nuttalli (n = 253), and Dermacentor silvarum (n = 204) ticks were collected by flagging from northeastern China, and detected for infection with Anaplasma, Ehrlichia, Babesia, and Hepatozoon spp. by using nested polymerase chain reaction assays and sequencing analysis. Anaplasma phagocytophilum was detected in all tick species, i.e., I. persulcatus (9.4%), H. longicornis (1.9%), H. concinna (6.5%), D. nuttalli (1.7%), and D. silvarum (2.3%); Anaplasma bovis was detected in H. longicornis (0.3%) and H. concinna (0.2%); Ehrlichia muris was detected in I. persulcatus (2.5%) and H. concinna (0.2%); Candidatus Neoehrlichia mikurensis was only detected in I. persulcatus (0.4%). The Ehrlichia variant (GenBank access number KU921424), closely related to Ehrlichia ewingii, was found in H. longicornis (0.8%) and H. concinna (0.2%). I. persulcatus was infected with Babesia venatorum (1.2%), Babesia microti (0.6%), and Babesia divergens (0.6%). Additionally, four Babesia sequence variants (GenBank access numbers 862303–862306) were detected in I. persulcatus, H. longicornis, and H. concinna, which belonged to the clusters formed by the parasites of dogs, sheep, and cattle (B. gibsoni, B. motasi, and B. crassa). Two Hepatozoon spp. (GenBank access numbers KX016028 and KX016029) associated with hepatozoonosis in Japanese martens were found in the collected ticks (0.1–3.1%). These findings showed the genetic variability of Anaplasma, Ehrlichia, Babesia, and Hepatozoon spp. circulating in ticks in northeastern China, highlighting the necessity for further research of these tick-associated pathogens and their role in human and animal diseases.

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Ehrlichia minasensis sp. nov., isolated from the tick Rhipicephalus microplus

Cited by 87 publications

References 21 publications

Assessment of a quantitative 5′ nuclease real-time polymerase chain reaction using groEL gene for Ehrlichia and Anaplasma species in rodents in Brazil

Assessment of a quantitative 5′ nuclease real-time polymerase chain reaction using groEL gene for Ehrlichia and Anaplasma species in rodents in Brazil

First detection of Ehrlichia minasensis in Hyalomma marginatum ticks collected from cattle in Corsica, France

Molecular Detection and Characterization of Zoonotic and Veterinary Pathogens in Ticks from Northeastern China

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