Complete Genome Sequence of Corynebacterium pseudotuberculosis Strain PA01, Isolated from Sheep in Pará, Brazil

Alves, Jorianne Thyeska Castro; Veras, Adonney Allan de Oliveira; Cavalcante, Ana Lidia Queiroz; Sá, Pablo Henrique Caracciolo Gomes de; Dias, Larissa Maranhão; Guimarães, Luís Carlos; Morais, Ezequiel; Silva, André G. M.; Azevedo, Vasco; Ramos, Rommel Thiago Jucá; Silva, Artur M. S.; Carneiro, Adriana Ribeiro

doi:10.1128/genomea.01664-15

Cited by 4 publications

(4 citation statements)

References 16 publications

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“…In the northern part of Brazil, according to the report of the Brazilian Institute of Geography and Statistics (IBGE) in 2015 ( http://www.ibge.gov.br/estadosat/temas.php?siglapa&temapecuaria2015 ), most sheep and goat herds in the region are from the State of Pará, which contribute 50% of the goat and 36% of the sheep herd breeding in the north of Brazil. However, the lack of data related to cases of CLA underestimates the actual prevalence of this disease in the state ( 6 ). This absence of information may be explained by deficiencies in notification of this disease in many countries ( 7 ).…”

Section: Genome Announcementmentioning

confidence: 99%

Whole-Genome Sequence of Corynebacterium pseudotuberculosis PA04, Isolated from the Lymph Node of a Sheep in the Amazon, Brazil

et al. 2017

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Section: Genome Announcementmentioning

confidence: 99%

Whole-Genome Sequence of Corynebacterium pseudotuberculosis PA04, Isolated from the Lymph Node of a Sheep in the Amazon, Brazil

et al. 2017

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“…Thus, studies are conducted regarding the importance of exoproteins in the pathogen-host interaction to identify new molecular targets to develop effective diagnostic tests with significant levels of sensitivity and specificity [ 15 ]. In this way, the sequencing of several genomes of C. pseudotuberculosis [ 16 , 17 ] contributed to finding potentially viable antigens before performing experiments to verify them. This technique increases the speed of antigen identification, decreases costs, increases vaccine strategies to obtain potential antigens for serological diagnosis.…”

Section: Introductionmentioning

confidence: 99%

In Silico Screening of Putative Corynebacterium pseudotuberculosis Antigens and Serological Diagnosis for Caseous Lymphadenitis in Sheep by Enzyme-Linked Immunosorbent Assay

Droppa-Almeida

Ferreira

Brito

et al. 2021

Veterinary Medicine International

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Corynebacterium pseudotuberculosis is the etiologic agent of Caseous Lymphadenitis (CLA), a disease leading to severe damage in sheep and goats farming due to the lack of serological diagnosis, treatment, and effective prophylaxis. In this context, several strategies in an attempt to discover new antigens to compose diagnosis assays or vaccines are fundamental. Therefore, this study aimed to use bioinformatics software to evaluate the critical chemical characteristics of unknown proteins of C. pseudotuberculosis by selecting them for heterologous expression in Escherichia coli. For this purpose, six protein sequences of ascorbate transporter subunit, UPF protein, MMPL family transporter, Ribonuclease, Iron ABC transporter domain-containing permease, and fimbrial subunit were obtained. In silico analyses were performed using amino acid sequences to access immunodominant epitopes and their antigenic and allergenic potential and physicochemical characterization. The expressed proteins were used as an antigen for serological diagnosis by ELISA. All proteins showed distinct immunodominant epitopes and potential antigenic characteristics. The only proteins expressed were PTS and Ribonuclease. In parallel, we expressed CP40 and all were used with ELISA antigen in 49 CLA positive sera and 26 CLA negative sera. The proteins alone showed 100% sensitivity and 96.2%, 92.3%, and 88.5% specificity for rPTS, rRibonuclease, and rCP40, respectively. When proteins were combined, they showed 100% sensitivity and 84.6%, 92.3%, 88.5%, and 92.3% specificity for rPTS/rCp40, rRibonuclease/rCP40, rPTS/rRibonuclease, and rPTS/rRibonuclease/rCP40, respectively. The results of this study show an excellent correlation of sensitivity and specificity with all proteins. None of the specificity values preclude the potential of rPTS, rRibonuclease, or rCP40 for use in ELISA diagnostic assays since the results of this work are superior to those of other studies on CLA diagnosis described in the literature.

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“…Followed by the emergence of isolation and sequencing of new C. pseudotuberculosis biovar ovis genomes, especially in the Northern and Northeastern regions of Brazil ( Alves et al, 2016 ; Muge et al, 2016 ), the development of strategies in order to comprehend and elucidate its genome features became bigger and faster, helping to understand biological processes, gene repertoire, gene regulation and PPI ( Nourani, Khunjush & Durmus, 2015 ). Despite this, several genes present in the genome of this pathogen still have unknown functions, being characterized as hypothetical proteins (HP).…”

Section: Introductionmentioning

confidence: 99%

In silico functional prediction of hypothetical proteins from the core genome of Corynebacterium pseudotuberculosis biovar ovis

Araújo

Blanco

Souza

et al. 2020

PeerJ

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Corynebacterium pseudotuberculosis is a pathogen of veterinary relevance diseases, being divided into two biovars: equi and ovis; causing ulcerative lymphangitis and caseous lymphadenitis, respectively. The isolation and sequencing of C. pseudotuberculosis biovar ovis strains in the Northern and Northeastern regions of Brazil exhibited the emergence of this pathogen, which causes economic losses to small ruminant producers, and condemnation of carcasses and skins of animals. Through the pan-genomic approach, it is possible to determine and analyze genes that are shared by all strains of a species—the core genome. However, many of these genes do not have any predicted function, being characterized as hypothetical proteins (HP). In this study, we considered 32 C. pseudotuberculosis biovar ovis genomes for the pan-genomic analysis, where were identified 172 HP present in a core genome composed by 1255 genes. We are able to functionally annotate 80 sequences previously characterized as HP through the identification of structural features as conserved domains and families. Furthermore, we analyzed the physicochemical properties, subcellular localization and molecular function. Additionally, through RNA-seq data, we investigated the differential gene expression of the annotated HP. Genes inserted in pathogenicity islands had their virulence potential evaluated. Also, we have analyzed the existence of functional associations for their products based on protein–protein interaction networks, and perform the structural prediction of three targets. Due to the integration of different strategies, this study can underlie deeper in vitro researches in the characterization of these HP and the search for new solutions for combat this pathogen.

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Complete Genome Sequence of Corynebacterium pseudotuberculosis Strain PA01, Isolated from Sheep in Pará, Brazil

Cited by 4 publications

References 16 publications

Whole-Genome Sequence of Corynebacterium pseudotuberculosis PA04, Isolated from the Lymph Node of a Sheep in the Amazon, Brazil

Whole-Genome Sequence of Corynebacterium pseudotuberculosis PA04, Isolated from the Lymph Node of a Sheep in the Amazon, Brazil

In Silico Screening of Putative Corynebacterium pseudotuberculosis Antigens and Serological Diagnosis for Caseous Lymphadenitis in Sheep by Enzyme-Linked Immunosorbent Assay

In silico functional prediction of hypothetical proteins from the core genome of Corynebacterium pseudotuberculosis biovar ovis

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