Complete Genome Sequence of Borrelia crocidurae

Elbir, Haitham; Gimenez, Grégory; Robert, Caroline; Bergström, Sven; Cutler, Sally J.; Raoult, Didier; Drancourt, Michel

doi:10.1128/jb.00118-12

Cited by 28 publications

(25 citation statements)

References 12 publications

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“…Reportedly complete chromosome and plasmid sequences are available for only three Old World species: B. duttonii, B. recurrentis, and B. crocidurae (24,25). The report on the genomes for B. duttonii and B. recurrentis focused on the chromosome and some of the smaller plasmids, with limited annotation of the features of the large linear plasmids of those species (24).…”

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confidence: 99%

Large Linear Plasmids of Borrelia Species That Cause Relapsing Fever

et al. 2013

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c Borrelia species of relapsing fever (RF) and Lyme disease (LD) lineages have linear chromosomes and both linear and circular plasmids. Unique to RF species, and little characterized to date, are large linear plasmids of ϳ160 kb, or ϳ10% of the genome. By a combination of Sanger and next-generation methods, we determined the sequences of large linear plasmids of two New World species: Borrelia hermsii, to completion of its 174-kb length, and B. turicatae, partially to 114 kb of its 150 kb. These sequences were then compared to corresponding sequences of the Old World species B. duttonii and B. recurrentis and to plasmid sequences of LD Borrelia species. The large plasmids were largely colinear, except for their left ends, about 27 kb of which was inverted in New World species. Approximately 60% of the B. hermsii lp174 plasmid sequence was repetitive for 6 types of sequence, and half of its open reading frames encoded hypothetical proteins not discernibly similar to proteins in the database. The central ϳ25 kb of all 4 linear plasmids was syntenic for orthologous genes for plasmid maintenance or partitioning in Borrelia species. Of all the sequenced linear and circular plasmids in Borrelia species, the large plasmid's putative partition/replication genes were most similar to those of the 54-kb linear plasmids of LD species. Further evidence for shared ancestry was the observation that two of the hypothetical proteins were predicted to be structurally similar to the LD species' CspA proteins, which are encoded on the 54-kb plasmids.

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Large Linear Plasmids of Borrelia Species That Cause Relapsing Fever

et al. 2013

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“…Their ORF names are BTA001 and BPA001, respectively. BHA007, BTA001, and BPA001 were also noted to be orthologous to similarly positioned ORFs on the large plasmids of the African species B. duttonii and B. recurrentis (45) as well as in the deposited genome of B. crocidurae (46). With identities to BHA007 of only 42 to 44%, the ORFs of African species were more distantly related to BHA007 than were those of the other North American species, which were 56 to 60% identical in sequence (see Table S2 in the supplemental material).…”

Section: Resultsmentioning

confidence: 99%

Fibronectin-Binding Protein of Borrelia hermsii Expressed in the Blood of Mice with Relapsing Fever

et al. 2014

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To identify and characterize surface proteins expressed by the relapsing fever (RF) agent Borrelia hermsii in the blood of infected mice, we used a cell-free filtrate of their blood to immunize congenic naive mice. The resultant antiserum was used for Western blotting of cell lysates, and gel slices corresponding to reactive bands were subjected to liquid chromatography-tandem mass spectrometry, followed by a search of the proteome database with the peptides. One of the immunogens was identified as the BHA007 protein, which is encoded by a 174-kb linear plasmid. BHA007 had sequence features of lipoproteins, was surface exposed by the criteria of in situ protease susceptibility and agglutination of Vtp ؊ cells by anti-BHA007 antibodies, and was not essential for in vitro growth. BHA007 elicited antibodies during experimental infection of mice, but immunization with recombinant protein did not confer protection against needle-delivered infection. Open reading frames (ORFs) orthologous to BHA007 were found on large plasmids of other RF species, including the coding sequences for the CihC proteins of Borrelia duttonii and B. recurrentis, but not in Lyme disease Borrelia species. Recombinant BHA007 bound both human and bovine fibronectin with K d (dissociation constant) values of 22 and 33 nM, respectively, and bound to C4-binding protein with less affinity. The distant homology of BHA007 and its orthologs to BBK32 proteins of Lyme disease species, as well as to previously described BBK32-like proteins in relapsing fever species, indicates that BHA007 is a member of a large family of multifunctional proteins in Borrelia species that bind to fibronectin as well as other host proteins.

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“…Relatively few genetic loci, such as the single-copy chromosomal 16S rRNA and flabB genes 39 and the 16S-23S ribosomal RNA intergenic spacer (IGS), have been used to discriminate between the various African relapsing fever borreliae. 40 Genetic comparisons 18,19 indicate that the 16S rRNA gene exhibits two to six nucleotide differences among the four cultured species; these differences are scattered along the whole 16S rRNA gene sequence for each species, resulting in a high overall sequence similarity of 99.7-99.9% between the four species. 41 Moreover, interrogation of GenBank database indicates that these signatures are not consistent over the 37 B. crocidurae 16S rRNA gene sequences recorded in GenBank, resulting in a 1% 16S rRNA gene sequence intraspecies variation.…”

Section: Clinical Featuresmentioning

confidence: 99%

“…19 Recent efforts to culture and sequence relapsing fever borreliae have provided new information for a reassessment of the diversity of these bacteria. In this review, we consider the molecular evidence of relapsing fever borreliae in hosts and ectoparasites in Africa and explore the diversity, geographical distribution, and vector association of these pathogens for Africa and travelers to Africa.…”

Section: Introductionmentioning

confidence: 99%

Relapsing Fever Borreliae in Africa

Elbir¹,

Raoult²,

Drancourt³

2013

The American Society of Tropical Medicine and Hygiene

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Abstract. The study of relapsing fever borreliae in Africa has long suffered from the use of non-specific laboratory tools for the direct detection of these spirochetes in clinical and vector specimens. Accordingly, Borrelia hispanica, Borrelia crocidurae, Borrelia duttonii, and Borrelia recurrentis have traditionally been distinguished on the basis of geography and vector and the unproven hypothesis that each species was exclusive to one vector. The recent sequencing of three relapsing fever Borrelia genomes in our laboratory prompted the development of more specific tools and a reappraisal of the epidemiology in Africa. Five additional potential species still need to be cultured from clinical and vector sources in East Africa to further assess their uniqueness. Here, we review the molecular evidence of relapsing fever borreliae in hosts and ectoparasites in Africa and explore the diversity, geographical distribution, and vector association of these pathogens for Africans and travelers to Africa.

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Complete Genome Sequence of Borrelia crocidurae

Cited by 28 publications

References 12 publications

Large Linear Plasmids of Borrelia Species That Cause Relapsing Fever

Large Linear Plasmids of Borrelia Species That Cause Relapsing Fever

Fibronectin-Binding Protein of Borrelia hermsii Expressed in the Blood of Mice with Relapsing Fever

Relapsing Fever Borreliae in Africa

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