Complete genome sequence of Bifidobacterium angulatum JCM 7096T isolated from human feces

Toh, Y.; Oshima, Koichiro; Nakano, Akiyo; Kiuchi, Misa; Kuroyanagi, Hiromi; Arakawa, Kiyoshi; Suda, Wataru; Honda, Kenya; Hattori, Masahira

doi:10.1016/j.jbiotec.2015.06.412

Cited by 5 publications

(2 citation statements)

References 8 publications

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“…However, it has been suggested that B. dentium possesses the ability to remove terminal fucose from HMOs [ 48 ]. Researchers found a smaller number of glycosyl hydrolase genes in the genomes of Bifidobacterium angulatum JCM 7096(T) and Bifidobacterium pseudocatenulatum JCLA3 [ 49 , 50 ]. The B. thermophilum genome contains a β-galactosidase gene, a finding that hints at its potential to degrade HMOs since β-galactosidase can participate in their metabolism; however, the exact mechanism remains uncharted [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

An In Vitro Colonic Fermentation Study of the Effects of Human Milk Oligosaccharides on Gut Microbiota and Short-Chain Fatty Acid Production in Infants Aged 0–6 Months

Li,

Lu,

Xue

et al. 2024

Foods

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The impact of five human milk oligosaccharides (HMOs)—2′-fucosyllactose (2FL), 3′-sialyllactose (3SL), 6′-sialyllactose (6SL), lacto-N-tetraose (LNT), and lacto-N-neotetraose (LNnT)—on the gut microbiota and short-chain fatty acid (SCFA) metabolites in infants aged 0–6 months was assessed through in vitro fermentation. Analyses of the influence of different HMOs on the composition and distribution of infant gut microbiota and on SCFA levels were conducted using 16S rRNA sequencing, quantitative real-time PCR (qPCR), and gas chromatography (GC), respectively. The findings indicated the crucial role of the initial microbiota composition in shaping fermentation outcomes. Fermentation maintained the dominant genera species in the intestine but influenced their abundance and distribution. Most of the 10 Bifidobacteria strains effectively utilized HMOs or their degradation products, particularly demonstrating proficiency in utilizing 2FL and sialylated HMOs compared to non-fucosylated neutral HMOs. Moreover, our study using B. infantis-dominant strains and B. breve-dominant strains as inocula revealed varying acetic acid levels produced by Bifidobacteria upon HMO degradation. Specifically, the B. infantis-dominant strain yielded notably higher acetic acid levels than the B. breve-dominant strain (p = 0.000), with minimal propionic and butyric acid production observed at fermentation’s conclusion. These findings suggest the potential utilization of HMOs in developing microbiota-targeted foods for infants.

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Section: Discussionmentioning

confidence: 99%

An In Vitro Colonic Fermentation Study of the Effects of Human Milk Oligosaccharides on Gut Microbiota and Short-Chain Fatty Acid Production in Infants Aged 0–6 Months

Li,

Lu,

Xue

et al. 2024

Foods

View full text Add to dashboard Cite

show abstract

“…We searched the typical genomes of 17 bifidobacterial species ( Schell et al., 2002 ; Sela et al., 2008 ; Ventura et al., 2009 ; Yasui et al., 2009 ; Turroni et al., 2010 ; O'Connell et al., 2011 ; Jans et al., 2013 ; Milani et al., 2013 , 2014 ; Chen et al., 2015 ; Morita et al., 2015a , 2015b , 2015c , 2015d ; Toh et al., 2015 ; Vazquez-Gutierrez et al., 2015 ) for orthologs of housekeeping sortase or SDPs, and found that BF1_0427 was conserved among all these species, whereas the SDPs, especially GHs ( Table 2 ), were specific to B. bifidum . GHs identified and biochemically characterized in B. bifidum so far include BF1_0298 ( Ashida et al., 2018 ), BF1_1449 ( Wakinaka et al., 2013 ), BF1_0299 ( Kiyohara et al., 2011 ), BF1_1506 ( Ashida et al., 2009 ), BF1_1579 ( Wada et al., 2008 ), BF1_0510 ( Katayama et al., 2004 , 2005 ), and BF1_0030 ( Shimada et al., 2015 ).…”

Section: Discussionmentioning

confidence: 99%