Complete Genome Sequence of Bacillus thuringiensis Serovar Sichuansis Strain MC28

Guan, Peng; Ai, Peng; Dai, Xiangyu; Zhang, Jing; Xu, Lizhi; Zhu, Jun; Li, Qiao; Deng, Qi; Li, Shuangcheng; Wang, Yunlong; Liu, Huannian; Wang, Lingxia; Li, Ping; Zheng, Anqi

doi:10.1128/jb.01861-12

Cited by 25 publications

(14 citation statements)

References 6 publications

(3 reference statements)

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“…We hypothesize that these toxins may be toxic to dipteran insects since they are contained within dipteran subnetworks. Actually, Cry29A was reported to synergize the toxicity of Cry11Bb against Aedes aegypti (Diptera: Culicidae), and Cry50Aa, Cry50Ba, Cry54Ba, Cry68Aa, and Cry70Aa were all found in some strains with high toxicity against mosquitoes ( 52 – 55 ). Similarly, in the coleopteran subnetwork, Cry26 and Cry28 had no reported toxicity; we suspect that they may target coleopteran hosts.…”

Section: Discussionmentioning

confidence: 99%

“…In CT-43 and HD-1, five toxins were encoded by genes from two different plasmids, with four of them on the larger one and the other on a smaller one, respectively ( 58 ). In MC28, 11 toxin genes were contained by three different plasmids ( 53 ), and in IS5056, nine toxins were encoded by genes on four different plasmids ( 59 ). The range of loci encoding toxins implies that multiple toxins in the same genome were gained by multiple independent HGT events.…”

Section: Discussionmentioning

confidence: 99%

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Comparative Genomics of Bacillus thuringiensis Reveals a Path to Specialized Exploitation of Multiple Invertebrate Hosts

Zheng

Gao

Liu

et al. 2017

mBio

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Understanding the genetic basis of host shifts is a key genomic question for pathogen and parasite biology. The Bacillus cereus group, which encompasses Bacillus thuringiensis and Bacillus anthracis, contains pathogens that can infect insects, nematodes, and vertebrates. Since the target range of the essential virulence factors (Cry toxins) and many isolates is well known, this group presents a powerful system for investigating how pathogens can diversify and adapt to phylogenetically distant hosts. Specialization to exploit insects occurs at the level of the major clade and is associated with substantial changes in the core genome, and host switching between insect orders has occurred repeatedly within subclades. The transfer of plasmids with linked cry genes may account for much of the adaptation to particular insect orders, and network analysis implies that host specialization has produced strong associations between key toxin genes with similar targets. Analysis of the distribution of plasmid minireplicons shows that plasmids with orf156 and orf157, which carry genes encoding toxins against Lepidoptera or Diptera, were contained only by B. thuringiensis in the specialized insect clade (clade 2), indicating that tight genome/plasmid associations have been important in adaptation to invertebrate hosts. Moreover, the accumulation of multiple virulence factors on transposable elements suggests that cotransfer of diverse virulence factors is advantageous in terms of expanding the insecticidal spectrum, overcoming insect resistance, or through gains in pathogenicity via synergistic interactions between toxins.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Comparative Genomics of Bacillus thuringiensis Reveals a Path to Specialized Exploitation of Multiple Invertebrate Hosts

Zheng

Gao

Liu

et al. 2017

mBio

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“…Because the genome data available for the B. cereus sensu lato group in NCBI include 50 B. thuringiensis , 161 B. cereus and 93 B. anthracis strains annotated according to previously reported insecticidal activities and serotypes, we divided the 50 B. thuringiensis strains into three groups: highly, generally and weakly toxic strains (12, 29 and 9 strains, respectively, Table 1 and Supplementary Table S2 ). The comparison showed that the B. thuringiensis strains with high insecticidal toxicity 10 12 19 had significantly larger genome sizes and were richer in plasmid content than the weakly toxic B. thuringiensis strains, and typical B. cereus , B. anthracis strains which are not toxic to insects (P < 0.05, T-test, Fig. 1A,B ).…”

Section: Resultsmentioning

confidence: 99%

“…These results suggested that B. thuringiensis possessed a complicated pathogenic mechanism. Due to the rapid development of high-throughput sequencing technology, many B. thuringiensis genomes have been completed in recent years 9 10 11 12 13 14 15 16 . However, with the exception of two draft genomes of Btk 17 18 , no complete genomes of the biopesticide production strains (especially Btk HD-1 and similar strains) are currently available.…”

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confidence: 99%

Genomic and transcriptomic insights into the efficient entomopathogenicity of Bacillus thuringiensis

Zhu

Peng

Wang

et al. 2015

Sci Rep

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Bacillus thuringiensis has been globally used as a microbial pesticide for over 70 years. However, information regarding its various adaptions and virulence factors and their roles in the entomopathogenic process remains limited. In this work, we present the complete genomes of two industrially patented Bacillus thuringiensis strains (HD-1 and YBT-1520). A comparative genomic analysis showed a larger and more complicated genome constitution that included novel insecticidal toxicity-related genes (ITRGs). All of the putative ITRGs were summarized according to the steps of infection. A comparative genomic analysis showed that highly toxic strains contained significantly more ITRGs, thereby providing additional strategies for infection, immune evasion, and cadaver utilization. Furthermore, a comparative transcriptomic analysis suggested that a high expression of these ITRGs was a key factor in efficient entomopathogenicity. We identified an active extra urease synthesis system in the highly toxic strains that may aid B. thuringiensis survival in insects (similar to previous results with well-known pathogens). Taken together, these results explain the efficient entomopathogenicity of B. thuringiensis. It provides novel insights into the strategies used by B. thuringiensis to resist and overcome host immune defenses and helps identify novel toxicity factors.

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“…During sporulation, the gram-positive bacterium Bacillus thuringiensis (Bt) produces crystalline inclusions consisting of δ-endotoxins (Cry or Cyt proteins) with insecticidal activity 1 . Genomic analysis has contributed to the identification of new genes coding for toxins that are active against different insect species including orders such as Lepidoptera, Diptera [2][3][4][5][6][7] , and Coleoptera 8 . Proteins with nematicidal [9][10][11] and molluscicidal 12 activities have also been described.…”

mentioning

confidence: 99%

Comparative genomic analysis and mosquito larvicidal activity of four Bacillus thuringiensis serovar israelensis strains

Alves

Melo

Oliveira

et al. 2020

Sci Rep

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Bacillus thuringiensis serovar israelensis (Bti) is used to control insect vectors of human and animal diseases. in the present study, the toxicity of four strains of Bti, named T0124, T0131, T0137, and T0139, toward Aedes aegypti and Culex quinquefasciatus larvae was analyzed. The T0131 strain showed the highest larvicidal activity against A. aegypti (Lc 50 = 0.015 µg/ml) and C. quinquefasciatus larvae (Lc 50 = 0.035 µg/ml) when compared to the other strains. Furthermore, the genomic sequences of the four strains were obtained and compared. These Bti strains had chromosomes sizes of approximately 5.4 Mb with GC contents of ~35% and 5472-5477 putative coding regions. Three small plasmids (5.4, 6.8, and 7.6 kb) and three large plasmids (127, 235, and 359 kb) were found in the extrachromosomal content of all four strains. the Snp-based phylogeny revealed close relationship among isolates from this study and other Bti isolates, and SNPs analysis of the plasmids 127 kb did not reveal any mutations in δ-endotoxins genes. This newly acquired sequence data for these Bti strains may be useful in the search for novel insecticidal toxins to improve existing ones or develop new strategies for the biological control of important insect vectors of human and animal diseases.

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Complete Genome Sequence of Bacillus thuringiensis Serovar Sichuansis Strain MC28

Cited by 25 publications

References 6 publications

Comparative Genomics of Bacillus thuringiensis Reveals a Path to Specialized Exploitation of Multiple Invertebrate Hosts

Comparative Genomics of Bacillus thuringiensis Reveals a Path to Specialized Exploitation of Multiple Invertebrate Hosts

Genomic and transcriptomic insights into the efficient entomopathogenicity of Bacillus thuringiensis

Comparative genomic analysis and mosquito larvicidal activity of four Bacillus thuringiensis serovar israelensis strains

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