Complete Genome Sequence of an Overattenuated Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus

Wu, Jianming; Chen, Nanhua; Han, Wei; Cao, Zhen; Deng, Xiaoyu; Wang, Lilin; Yu, Xiaofang; Zhou, Zhi; Li, Xiangdong; Shi, Jishu; Tian, Kegong

doi:10.1128/jvi.00710-12

Cited by 6 publications

(6 citation statements)

References 12 publications

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“…Our data and the previously reported data indicate that the HP-PRRSV variant continues prevailing and accelerating evolution in China (8,9,10). Results of the SD16 sequence will enhance not only our understanding of the PRRSV evolutionary mechanism but also that of arterivirus.…”

supporting

confidence: 49%

Complete Genome Sequence of a Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Variant

Wang

Zhao

Liang

et al. 2012

J Virol

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Following the 2006 outbreaks of the highly pathogenic porcine reproductive and respiratory syndrome, the causative agent was identified as the highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV). To investigate whether the HP-PRRSV variant continues circulating and accelerating evolution, we sequenced and analyzed the complete genome of the identified HP-PRRSV field strain SD16. The sequence data indicate that the HP-PRRSV variant continues to prevail and accelerate evolution, especially in the nonstructural protein.

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supporting

confidence: 49%

Complete Genome Sequence of a Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Variant

Wang

Zhao

Liang

et al. 2012

J Virol

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“…Genomic analysis showed that nearly all of emerging HP-PRRSV isolates share a unique discontinuous deletion of 30 amino acids (aa) in nonstructural protein 2 (Nsp2). However, a number of newly isolated PRRSV variants with different deletions or recombinants was reported in China and the HP-PRRSV variant continues prevailing and accelerating evolution in China [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

A novel porcine reproductive and respiratory syndrome virus vector system that stably expresses enhanced green fluorescent protein as a separate transcription unit

Wang

Huang

Kong

et al. 2013

Vet Res

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Here we report the rescue of a recombinant porcine reproductive and respiratory syndrome virus (PRRSV) carrying an enhanced green fluorescent protein (EGFP) reporter gene as a separate transcription unit. A copy of the transcription regulatory sequence for ORF6 (TRS6) was inserted between the N protein and 3′-UTR to drive the transcription of the EGFP gene and yield a general purpose expression vector. Successful recovery of PRRSV was obtained using an RNA polymerase II promoter to drive transcription of the full-length virus genome, which was assembled in a bacterial artificial chromosome (BAC). The recombinant virus showed growth replication characteristics similar to those of the wild-type virus in the infected cells. In addition, the recombinant virus stably expressed EGFP for at least 10 passages. EGFP expression was detected at approximately 10 h post infection by live-cell imaging to follow the virus spread in real time and the infection of neighbouring cells occurred predominantly through cell-to-cell-contact. Finally, the recombinant virus generated was found to be an excellent tool for neutralising antibodies and antiviral compound screening. The newly established reverse genetics system for PRRSV could be a useful tool not only to monitor virus spread and screen for neutralising antibodies and antiviral compounds, but also for fundamental research on the biology of the virus.

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“…In contrast with the recombinant or overattenuated HP–PRRSV strain as has been reported previously (5, 9, 10), the YN-2011 strain could induce typical clinical symptoms. However, the results identify the viewpoint that the 30-amino-acid (aa) deletion in NS2 is not related to the virulence of the virus (11).…”

Section: Genome Announcementmentioning

confidence: 46%

“…Since its appearance in southern China in 2006, highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) has the characteristic of a discontinuous 30-amino-acid (aa) deletion in nonstructural protein 2 (NS2) (5, 6). Under the strong immune pressures caused by the current control strategy, PRRSVs are geared for rapid variation through mutation or recombination (7–9), resulting in new isolates with different levels of pathogenicity and virulence. In this study, we report a complete genomic sequence of the local novel natural recombinant PRRSV, characterized by the lack of the NS2 deletion, but which also can cause serious clinical symptoms, including death, which were isolated from vaccinated pig farms in the Yunnan province of southwest China.…”

Section: Genome Announcementmentioning

confidence: 99%

Complete Genome Sequence of a Novel Natural Recombinant Porcine Reproductive and Respiratory Syndrome Virus Isolated from a Pig Farm in Yunnan Province, Southwest China

et al. 2013

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Complete Genome Sequence of an Overattenuated Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus

Cited by 6 publications

References 12 publications

Complete Genome Sequence of a Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Variant

Complete Genome Sequence of a Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Variant

A novel porcine reproductive and respiratory syndrome virus vector system that stably expresses enhanced green fluorescent protein as a separate transcription unit

Complete Genome Sequence of a Novel Natural Recombinant Porcine Reproductive and Respiratory Syndrome Virus Isolated from a Pig Farm in Yunnan Province, Southwest China

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