Complete genome sequence of a novel mycovirus isolated from the phytopathogenic fungus Corynespora cassiicola in China

Wang, Yanfen; Zhao, H. S.; Xue, Caiying; Xu, Chao; Geng, Yuehua; Zang, Rui; Guo, Yuxia; Wu, Haiyan; Zhang, Meng

doi:10.1007/s00705-020-04723-2

Cited by 9 publications

(3 citation statements)

References 15 publications

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“…Based on the phylogenetic tree constructed in our present study (Figure 4D) as well as several recent reports [37,75], RsRV11 together with the RfMV cluster in the proposed family Bipartitiviridae, indicating that RfMV has a distinct relationship with members of the family Megabirnaviridae. In fact, RfMV contains one large dsRNA (9907 nt) as a genome encoding two proteins, which is different from the genomes of representative members within the family Megabirnaviridae (containing two large dsRNAs as a genome) [76][77][78] or the proposed family Bipartitiviridae (containing two dsRNAs with approximate 2000 nt in length) [79,80]. Thus, further studies on the evolutionary relationship between the family Megabirnaviridae and the proposed family Bipartitiviridae should be considered in the future.…”

Section: Discussionmentioning

confidence: 99%

Six Novel Mycoviruses Containing Positive Single-Stranded RNA and Double-Stranded RNA Genomes Co-Infect a Single Strain of the Rhizoctonia solani AG-3 PT

Zhang

et al. 2022

Viruses

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Six novel mycoviruses that collectively represent the mycovirome of Rhizoctonia solani anastomosis group (AG)-3 PT strain ZJ-2H, which causes potato black scurf, were identified through metatranscriptome sequencing and putatively designated as Rhizoctonia solani fusarivirus 4 [RsFV4, positive single-stranded RNA (+ssRNA)], Rhizoctonia solani fusarivirus 5 (RsFV5, +ssRNA), Rhizoctonia solani mitovirus 40 (RsMV40, +ssRNA), Rhizoctonia solani partitivirus 10 [RsPV10, double-stranded RNA (dsRNA)], Rhizoctonia solani partitivirus 11 (RsPV11, dsRNA), and Rhizoctonia solani RNA virus 11 (RsRV11, dsRNA). Whole genome sequences of RsFV4, RsMV40, RsPV10, RsPV11, and RsRV11, as well as a partial genome sequence of RsFV5, were obtained. The 3’- and 5’- untranslated regions of the five mycoviruses with complete genome sequences were folded into stable stem-loop or panhandle secondary structures. RsFV4 and RsFV5 are most closely related to Rhizoctonia solani fusarivirus 1 (RsFV1), however, the first open reading frame (ORF) of RsFV4 and RsFV5 encode a hypothetical protein that differs from the first ORF of RsFV1, which encodes a helicase. We confirmed that RsPV10 and RsPV11 assemble into the spherical virus particles (approximately 30 nm in diameter) that were extracted from strain ZJ-2H. This is the first report that +ssRNA and dsRNA viruses co-infect a single strain of R. solani AG-3 PT.

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Section: Discussionmentioning

confidence: 99%

Six Novel Mycoviruses Containing Positive Single-Stranded RNA and Double-Stranded RNA Genomes Co-Infect a Single Strain of the Rhizoctonia solani AG-3 PT

Zhang

et al. 2022

Viruses

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“…However, Fungal viruses that infect C. cassiicola are rarely reported. So far, only one unassigned dsRNA mycoviruse infecting C. cassiicola has been reported [19]. As far as we know, this is the rst study on the Victorivirus genus infected with C. cassiicola.…”

Section: Introductionmentioning

confidence: 93%

Molecular characterization of a novel victorivirus infecting Corynespora cassiicola

Liu

et al. 2022

Arch Virol

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One victorivirus was detected in the isolate of Corynespora cassiicola strains 20180909-03, which was named Corynespora cassiicola victorivirus 1 (CcVV1). The whole-genome sequence of the virus was sequenced and identi ed. The CcVV1 genome is 5140 nt and contains 56.87%GC with two large open reading frames (ORFs) overlapping at the tetranucleotide AUGA. The two ORFs were predicted to encode coat protein (CP) and RNA-dependent RNA polymerase (RdRp) respectively, which were conservative in dsRNA fungal viruses of the family Totiviridae. Conservative domains comparison and phylogenetic analysis of the deduced amino acid sequence of RdRp and CP showed that CcVV1 was a new virus of the Victorivirus genus. As far as we know, it is the rst report of a genomic sequence of the genus Victorivirus infecting Corynespora cassiicola.

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“…A series of bipartite mycoviruses, an unassigned group, have been identi ed from various fungi that consist of two double-stranded RNA (dsRNA) segments, where the RdRP sequences have more similarity to the RdRPs of proposed Unirnaviruses (mono-segment genomes) than partitiviruses (two-segment genomes). To data, 23 bipartite mycovirus have been reported including Cryphonectria parasitica bipartite mycovirus 1, Lactarius rufus RNA virus 1, and Penicillium aurantiogriseum bipartite virus 1et al [8][9][10][11].…”

Section: Main Textmentioning

confidence: 99%

Molecular Characterization of a Novel Mycovirus Infecting the Entomopathogenic Fungus Beauveria Bassiana

Shi

Wang

et al. 2021

Preprint

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The entomopathogenic fungus Beauveria bassiana is used worldwide for its biological control. Seven dsRNA segments were detected from a single Beauveria bassiana strain RCEF 1446. High-throughput sequencing indicated the presence of three mycoviruses in the infected sample. Two known mycoviruses were identified as Beauveria bassiana Victorivirus 1 and Beauveria bassiana polymycovirus 1, and the novel mycovirus was designated as Beauveria bassiana bipartite mycovirus 1 (BbBV1). The complete sequence of the BbBV1 is described here. The mycovirus contains two dsRNA segments. The first dsRNA is 2,026 bp in length and encoding a RNA-dependent RNA polymerase (RdRp) (68.54 kDa), while the second segment encoding a hypothetical protein (35.55 kDa) of unknown function, was 1,810 bp in length. Moreover, the RdRp protein sequences showed the highest identity of 62.89% to Corynespora cassiicola bipartite mycovirus 1. Phylogenetic analysis of the RdRp reveals that the virus represents a distinct lineage of unassigned dsRNA mycoviruses infecting fungi.

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Complete genome sequence of a novel mycovirus isolated from the phytopathogenic fungus Corynespora cassiicola in China

Cited by 9 publications

References 15 publications

Six Novel Mycoviruses Containing Positive Single-Stranded RNA and Double-Stranded RNA Genomes Co-Infect a Single Strain of the Rhizoctonia solani AG-3 PT

Six Novel Mycoviruses Containing Positive Single-Stranded RNA and Double-Stranded RNA Genomes Co-Infect a Single Strain of the Rhizoctonia solani AG-3 PT

Molecular characterization of a novel victorivirus infecting Corynespora cassiicola

Molecular Characterization of a Novel Mycovirus Infecting the Entomopathogenic Fungus Beauveria Bassiana

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