Complete Genome Sequence of a Recombinant Marek's Disease Virus Field Strain with One Reticuloendotheliosis Virus Long Terminal Repeat Insert

Su, Shuai; Cui, Ning; Cui, Zhizhong; Zhao, Peng; Li, Yanpeng; Ding, Jiabo; Xuan, Dong

doi:10.1128/jvi.02583-12

Cited by 31 publications

(23 citation statements)

References 17 publications

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“…An infectious BAC-derived vvMDV strain GX0101 (Sun et al, 2009Cui et al, 2010;Su et al, 2012) was used as the parental virus to construct the miRNA-deletion mutants.…”

Section: Methodsmentioning

confidence: 99%

The significance of the individual Meq-clustered miRNAs of Marek’s disease virus in oncogenesis

Teng

Zhao

Sun

et al. 2015

Journal of General Virology

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Marek's disease virus (MDV) is an important oncogenic alphaherpesvirus that induces rapid-onset T-cell lymphomas in its natural hosts. The Meq-clustered miRNAs encoded by MDV have been suggested to play potentially critical roles in the induction of lymphomas. Using the technique of bacterial artificial chromosome mutagenesis, we have presently constructed a series of specific miRNA-deleted mutants and demonstrate that these miRNAs are not essential for replication of MDV and have no effects on the early cytolytic or latent phases of the developing disease. However, compared to the parental GX0101, mortality of birds infected with the mutants GXDmiR-M2, GXDmiR-M3, GXDmiR-M5, GXDmiR-M9 and GXDmiR-M12 was reduced from 100 % to 18 %, 30 %, 48 %, 24 % and 14 %, coupled with gross tumour incidence reduction from 28 % to 8 %, 4 %, 12 %, 8 % and 0 %, respectively. Our data confirm that except for mdv1-miR-M4, the other Meq-clustered miRNAs also play critical roles in MDV oncogenesis. Further work will be needed to elucidate the miRNA-mediated regulatory mechanisms that trigger the development of MD lymphomas.

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“…An infectious BAC-derived vvMDV strain GX0101 (Sun et al, 2009Cui et al, 2010;Su et al, 2012) was used as the parental virus to construct the miRNA-deletion mutants.…”

Section: Methodsmentioning

confidence: 99%

The significance of the individual Meq-clustered miRNAs of Marek’s disease virus in oncogenesis

Teng

Zhao

Sun

et al. 2015

Journal of General Virology

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“…In the case of avian influenza (AIV) ducks are natural recombination vessels which cause the emergence of more virulent and transmissible AIV strains. Meanwhile, new poultry viruses may also originate from recombination between Marek's disease virus (MDV) and reticuloendotheliosis virus (REV) (Su et al 2012). A previous report on the occurrence of a novel duck reovirus strain from China shows the emergence of a duck reovirus causing 40% mortality in birds of various ages ).…”

Section: Discussionmentioning

confidence: 99%

Occurrence of reovirus infection in Muscovy ducks (Cairina moschata) in south western Poland

Woźniakowski¹,

Samorek-Salamonowicz²,

Gaweł³

2014

Polish Journal of Veterinary Sciences

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During the summer 2012 an incidence of high mortality, above 44 percent, in two flocks of Muscovy ducklings in Poland was noted. The clinical signs included considerable weight loss and inability to walk.During the post-mortem evaluations dehydration and enteritis, gouty kidneys as well as hemorrhagic liver and spleen lesions were found. The laboratory diagnosis included agar gel precipitation assay (AGP) as well as polymerase chain reaction (PCR) or reverse transcription PCR for the presence of goose parvovirus (GPV), duck circovirus (DuCV), duck reovirus (DRV) and avian reovirus (ARV). Interestingly, the examinations performed by AGP showed partial reactivity of liver homogenates from Muscovy ducklings with chicken S1133 antiserum. The presence of duck reovirus RNA was also detected by real-time RT-PCR targeting the chicken reovirus sigma NS fragment, while the sequencing showed major similarity to chicken S1133, 1733, GX/2010/1 and TARV-MN2 reovirus strains. The virus sequence was also related to a previously isolated TH11 strain from Muscovy ducks in China.Further study is needed in order to explain the particular epidemiology of the reovirus infection of Muscovy ducklings.

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“…Following deletion of the REV LTR sequence from GX0101, pathogenicity was increased slightly, while horizontal transmission capacity was decreased . Genomic sequencing has confirmed that a single REV LTR insert resides in the genome of GX0101 and is located upstream of the sorf2 gene (Su et al, 2012. Unlike the artificial constructed virus strain rMd5-RM1-LTR, in which the REV LTR insert was particularly unstable, existing for only one week after infection of chickens (Mays et al, 2012), the inserted REV LTR fragment in GX0101 remained stable following 20 passages in chickens.…”

Section: Discussionmentioning

confidence: 93%

“…Gene sequencing of a bacterial artificial chromosome clone (BAC) of the GX0101 strain (Sun et al, 2009) further confirmed that a single REV LTR was inserted into the sorf1 gene of GX0101 (Su et al, 2012. Analysis of BAC clones of GX0101 strain containing the REV LTR and LTR-deleted virus (GX0101ΔLTR) demonstrated that retention of the REV LTR in the MDV genome decreased the pathogenicity, but increased the potential for horizontal transmission (Sun et al, 2009.…”

Section: Introductionmentioning

confidence: 89%

Influence of avian leukosis virus long terminal repeat on biological activities of Marek's disease virus

Sun

Cui

et al. 2015

Avian Pathology

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Complete Genome Sequence of a Recombinant Marek's Disease Virus Field Strain with One Reticuloendotheliosis Virus Long Terminal Repeat Insert

Cited by 31 publications

References 17 publications

The significance of the individual Meq-clustered miRNAs of Marek’s disease virus in oncogenesis

The significance of the individual Meq-clustered miRNAs of Marek’s disease virus in oncogenesis

Occurrence of reovirus infection in Muscovy ducks (Cairina moschata) in south western Poland

Influence of avian leukosis virus long terminal repeat on biological activities of Marek's disease virus

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